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Plant responding low-phosphor and high-salt stress protein, encoding gene and uses thereof

A technology for encoding genes and plants, which can be applied to proteins in response to low-phosphorus and high-salt stress in plants and their encoding genes and applications, and can solve problems such as unclear effects.

Inactive Publication Date: 2009-08-19
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is now known that the Arabidopsis genome contains more than 1,500 genes encoding transcription factors, including 74 WRKY genes. The role of these WRKY genes in plant responses to low phosphorus and high salt stress is still unclear

Method used

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  • Plant responding low-phosphor and high-salt stress protein, encoding gene and uses thereof
  • Plant responding low-phosphor and high-salt stress protein, encoding gene and uses thereof
  • Plant responding low-phosphor and high-salt stress protein, encoding gene and uses thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Example 1. Acquisition of proteins and genes encoding them in response to low-phosphorus and high-salt stresses in plants

[0035] Total RNA was extracted from 10-day-old Arabidopsis (Columbia type) seedlings and reverse-transcribed into cDNA. Using the cDNA as a template, a pair of primers respectively provided with KpnI and Sa cI restriction sites were used for PCR amplification to obtain the full-length cDNA of drought tolerance related genes. The primer sequences are as follows:

[0036] Primer1: 5'-ggtaccATGGACAGAGGATGGTCTGGTCTC-3'(KpnI);

[0037] Primer2: 5'-gagctcCTATTGATTTTTGTTGTTTCCTTCGCCGTCGT-3' (SacI).

[0038] The high-fidelity Pyrobest DNA polymerase from Takara Company was used for amplification. The amplification system was 50 μL, containing 5.0 μL of 10×Pyrobest PCR buffer, 1.0 μL of 10 mM dNTP mix, 1.0 μL of each primer, 0.5 μL of Pyrobest enzyme, and 3.0 μL of template. (0.1ug), supplemented with sterilized ultrapure water to 50 μL, and the reaction...

Embodiment 2

[0045] Example 2. Functional verification of proteins and genes encoding them in response to low phosphorus and high salt stress in plants

[0046] 1. Obtainment of AtLPST overexpression plants (35S::ATLPST-9)

[0047] The recombinant vector pMD18-AtLPST containing the AtLPST gene obtained in Example 1 and the pBIB-Super vector (the construction method of the pBIB-Super vector: utilize primers aagcttGTGGGCCTGTGGTCTCAAGAT and tctagaCTAGAGTCGATTTGGT from plasmid pMSP-1 (Ni M, Cui D, Einstein J, NarasimhuluS, VergaraC, Gelvin SB.Strenght and tissue specificity of chimaericpromoters derived from the octopine and mannopinesysthase genes.19957: 661-676.) amplified a 1.1kb super promoter sequence; the amplified super promoter was used Double digestion with HindIII and XbaI, recover the super promoter fragment, and insert it into the HindIII and XbaI restriction sites of plasmid pBIB (Becher D. Binary vectors which allow the exchange of plant selectable markers and reportergenes. Nucl...

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Abstract

The invention discloses protein for plant response to low-phosphorus stress and high-salt stress, a coding gene and application thereof. The protein is protein which has the following amino acid sequences: 1) the protein which has the amino acid residue sequence of SEQ ID No.2 in a sequence table; and 2) the protein which causes the amino acid residue sequence of the SEQ ID No.2 in the sequence table to be replaced and / or lose and / or add by one or more amino acid residues, has functions of improving the plant sensitivity to low-phosphorus stress and high-salt stress and is derived from the SEQ ID No.2. The gene can be used for cultivating new plant varieties with low-phosphorus resistance and high-salt resistance, as well as indicator plants which are sensitive to low phosphorus and high salt.

Description

technical field [0001] The present invention relates to a protein for plant response to low phosphorus and high salt stress and its coding gene and application, in particular to a transcription factor from Arabidopsis thaliana that regulates plant response to low phosphorus and high salt stress, its coding gene and its application . Background technique [0002] The food problem is one of the major problems facing the world today. Soil phosphorus deficiency and salinization are two important limiting factors limiting agricultural production. Phosphate rock is a non-renewable resource, and a large amount of phosphate fertilizer will cause environmental pollution; saline soil is a kind of low-yield soil worldwide, even barren land. Therefore, understanding the response mechanism of plants to low-phosphorus and high-salt stress, and improving the tolerance of plants to low-phosphorus and high-salt stress have become an important research work on how to further increase crop y...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N5/10C12N1/00C12N15/11C12N15/82C12R1/91
Inventor 陈益芳武维华李立芹孔佑涵徐谦
Owner CHINA AGRI UNIV
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