Stem tip detoxification method for sweet potato virus seedling

A technology of sweet potato virus and seedling stems, which is applied in the field of virus-free stem tips of sweet potato virus seedlings, and can solve problems such as inability to apply the culture system

Inactive Publication Date: 2015-03-25
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But this method cannot be applicable to the culture system of the sweet potato shoot tip c...

Method used

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  • Stem tip detoxification method for sweet potato virus seedling
  • Stem tip detoxification method for sweet potato virus seedling
  • Stem tip detoxification method for sweet potato virus seedling

Examples

Experimental program
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Effect test

Embodiment 1

[0042] The sweet potato variety used in this example is cotton potato early autumn, which comes from the Potato Crops Research Institute of Southwest University.

[0043] A method for detoxifying the stem tip of sweet potato virus seedlings, the specific steps are as follows:

[0044] (1) Material cultivation: select and harvest potato pieces that have carried sweet potato feathery mottle virus, wash the surface and use 32mg / kg GA 3 Soak the seeds for 28 minutes, cover them with sterilized wet sand and bury them in the dark at 26°C to accelerate germination. When the buds emerge from the sand surface, heat them at 39°C for 7 days;

[0045] (2) Disinfection of materials: cut vigorously growing 1.8cm terminal buds, cut off fully expanded leaves on an ultra-clean workbench, and then sterilize; when sterilizing, soak the materials in 75% ethanol for 28 seconds, aseptic Rinse once with water, then use 0.1% HgCl 2 Soak for 9 minutes, rinse with sterile water 4 times;

[0046] (3)...

Embodiment 2

[0051] The sweet potato variety used in this example is Nanshu 88, which comes from the Potato Crops Research Institute of Southwest University.

[0052] A method for detoxifying the stem tip of sweet potato virus seedlings, the specific steps are as follows:

[0053] (1) Material cultivation: select and harvest potato pieces that have carried sweet potato G virus, wash the surface and use 30mg / kg GA 3 Soak the seeds for 25 minutes, cover them with sterilized wet sand and bury them in the dark at 25°C to accelerate germination. When the buds emerge from the sand surface, heat them at 38°C for 7 days;

[0054] (2) Disinfection of materials: clip the vigorously growing 1.5 terminal buds, cut off the fully expanded leaves on the ultra-clean workbench, and then sterilize; when sterilizing, the materials are first soaked in 75% ethanol for 25 seconds, then sterile water Rinse once, then use HgCl with a mass fraction of 0.1% 2 Soak for 8 minutes, rinse with sterile water 3 times; ...

Embodiment 3

[0059] The sweet potato variety used in this example is Chuanshu 101, which comes from the Potato Crops Research Institute of Southwest University.

[0060] A method for detoxifying the stem tip of sweet potato virus seedlings, the specific steps are as follows:

[0061] (1) Material cultivation: select and harvest potato pieces that have carried potato plume mottle virus and sweet potato G virus, wash the surface and use 35mg / kg GA 3 Soak the seeds for 30 minutes, cover them with sterilized wet sand and bury them in the dark at 27°C to accelerate germination. When the buds emerge from the sand surface, heat them at 40°C for 8 days;

[0062] (2) Disinfection of materials: cut vigorously growing 2.0cm terminal buds, cut off fully expanded leaves on an ultra-clean workbench, and then sterilize; when sterilizing, soak the materials in 75% ethanol for 30 seconds, sterile Rinse once with water, then use 0.1% HgCl 2 Soak for 10 minutes, rinse with sterile water 5 times;

[0063] ...

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Abstract

The invention relates to a stem tip detoxification and cultivation method for a sweet potato virus seedling. The method comprises the following steps: (1) taking a sweet potato block infected by sweet potato feathery mottle virus and/or sweet potato G virus; cultivating the sweet potato block indoors to sprout; (2) shearing scissoring terminal buds growing to 1.5-2.0 cm for disinfection; (3) peeling off the stem tips with two leaf primordiums in a sterile manner among the terminal buds as explants for vaccination cultivation to obtain regeneration plants; (4) conducting 2-3 times of successive transfer cultivation on the regeneration plants obtained in the step (3) to build a strain; conducting inspection by adopting an indicator plant method and a cellulose nitrate membrane ELISA in sequence to obtain a detoxified strain; (5) proliferating the detoxified strain quickly to obtain the detoxification seedling. The method can quickly build cultivation system suitable for stem tip cultivation and rapid propagation detoxification seedlings of majority of sweet potato varieties/strains.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the morphological structure and tissue culture technology of sweet potato shoot tip meristem, that is, to a method for detoxifying the shoot tip of sweet potato virus seedlings. Background technique [0002] Sweet potato (Ipomoea batatas L.) is an important food, feed and industrial raw material crop, which has the advantages of high yield, rich nutrition, strong adaptability and good stability. The currently promoted sweet potato varieties are generally sexual hybrid clones, which are highly heterozygous genetically. Therefore, it is required to use potato blocks for asexual reproduction in production to maintain excellent seed properties. In the production of sweet potato, the potato pieces are kept as seeds and asexually reproduced every year. Once the seed potatoes are infected by viral diseases, the viral diseases will accumulate and spread, resulting in a decrease i...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 黄远新王季春张凯唐道彬何凤发吕长文赵勇刘勋
Owner SOUTHWEST UNIVERSITY
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