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Cultivation method of moss for biological monitoring

A technology of biological monitoring and cultivation methods, applied in botany equipment and methods, cultivation, application, etc., can solve the problems of small comparability and achieve the effect of ensuring accuracy and high sensitivity

Active Publication Date: 2022-04-19
SOUTHWEAT UNIV OF SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is extremely challenging to separate, extract, and identify microplastics in natural environments and complex matrices. At present, there is still no unified method for microplastic detection, and the comparability of different experimental data is small. Detection There may be more undetected smaller microplastics, but these small microplastic particles may be more biologically significant

Method used

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  • Cultivation method of moss for biological monitoring
  • Cultivation method of moss for biological monitoring
  • Cultivation method of moss for biological monitoring

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] A kind of cultivation method of moss for biological monitoring of the present embodiment comprises the following steps:

[0052] Step 1, collection size is 25cm * 30cm big gray moss, cut off the big gray moss rhizome, the collected big gray moss is sieved for the first time, the sieve mesh number is 300 orders, and sowing for the first time; Cut off the rhizoid that grows gray moss after sowing for the first time, carry out the second sieve, the sieve mesh number is 500 orders;

[0053] Step 2, prepare the moss culture solution, use the moss culture solution to sow and cultivate the moss for the second time, and obtain the protonema; wherein, the preparation method of the moss culture solution comprises the following steps:

[0054] Step S21, in parts by weight, weigh 1g magnesium sulfate heptahydrate, 12g calcium nitrate tetrahydrate, 2g potassium nitrate, 1g sodium β-glycerophosphate pentahydrate, 10g trimethylolmethylaminopropanesulfonic acid, 0.1g ethylenediamine D...

Embodiment 2

[0074] A kind of cultivation method of moss for biological monitoring of the present embodiment comprises the following steps:

[0075] Step 1, collection size is 30cm * 70cm big gray moss, cut off the big gray moss rhizome, the collected big gray moss is sieved for the first time, the sieve mesh number is 400 orders, and the first sowing is carried out; Cut off the rhizoid that grows gray moss after sowing for the first time, carry out the second sieve, the sieve mesh number is 500 orders;

[0076] Step 2, prepare the moss culture solution, use the moss culture solution to sow and cultivate the moss for the second time, and obtain the protonema; wherein, the preparation method of the moss culture solution comprises the following steps:

[0077] Step S21, by weight, weigh 2g magnesium sulfate heptahydrate, 13g calcium nitrate tetrahydrate, 3g potassium nitrate, 2g sodium β-glycerophosphate pentahydrate, 20g trimethylolmethylaminopropanesulfonic acid, 0.2g ethylenediamine Diso...

Embodiment 3

[0093] A kind of cultivation method of moss for biological monitoring of the present embodiment comprises the following steps:

[0094] Step 1, collection size is 60cm * 90cm big gray moss, cut off the rhizome of big gray moss, the collected big gray moss is sieved for the first time, the sieve mesh number is 500 orders, and sowing for the first time; Cut off the rhizoids that grow out of gray moss after sowing for the first time, carry out the second sieve, and the sieve mesh number is 600 orders;

[0095] Step 2, prepare the moss culture solution, use the moss culture solution to sow and cultivate the moss for the second time, and obtain the protonema; wherein, the preparation method of the moss culture solution comprises the following steps:

[0096] Step S21, by weight, weigh 4g magnesium sulfate heptahydrate, 15g calcium nitrate tetrahydrate, 4g potassium nitrate, 2g sodium β-glycerophosphate pentahydrate, 40g trimethylolmethylaminopropanesulfonic acid, 0.2g ethylenediami...

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Abstract

The invention discloses a cultivation method of moss for biological monitoring, which comprises the following steps: collecting a whole piece of moss, cutting off the rhizoid of the moss, sieving the collected whole piece of moss for the first time, and sowing for the first time; cutting off rhizoid with moss after first-time sowing, performing second-time sieving, and preparing for second-time sowing; a moss culture solution is prepared, the moss culture solution is used for conducting second-time sowing and cultivation on moss, and protonemata is obtained; preparing a moss attachment matrix and an attachment matrix skeleton; the protonemata obtained in the second step is sown and attached to a moss attachment substrate, the protonemata differentiates on the moss attachment substrate to generate gametophytes, and the gametophytes grow on the moss attachment substrate to form target moss. The moss for biological monitoring cultivated by the invention has high sensitivity to micro-plastics in the environment, can effectively monitor the micro-plastics in the environment, and can be used as a preferred environmental micro-plastic pollution indication plant.

Description

technical field [0001] The invention belongs to the technical field of environmental protection and moss cultivation, and more specifically, the invention relates to a method for cultivating mosses for biological monitoring. Background technique [0002] In 1907, Baekeland discovered phenolic resin, which opened the door to the era of plastics, but at the same time, a large amount of plastic waste accumulated in the environment, potentially threatening human health and the function of the ecosystem [1] . More than 80% of the plastics currently produced are thermoplastic polymers with high molecular weight chains by polymerizing low molecular weight monomers (such as ethylene and propylene). Few of the commonly used plastics are biodegradable, so they can be stored in landfills. Or the accumulation in the natural environment can exist for hundreds or even thousands of years, and it exists widely in the atmosphere, ocean, soil, fresh water and other natural environments. Und...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/00A01G24/10A01G24/25A01G24/28A01G24/30A01G24/46A01G31/02
CPCA01G31/00A01G24/10A01G24/25A01G24/28A01G24/30A01G24/46A01G31/02Y02P60/21
Inventor 陈珂张济阳刘李岚类延宝李欣然倪甜甜梅思双
Owner SOUTHWEAT UNIV OF SCI & TECH
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