Bambusa multiplex transcription factor BmMYB83 gene and application thereof

A transcription factor and gene technology, applied in the filial piety bamboo transcription factor BmMYB83 gene and its application field, can solve problems such as almost blank research

Active Publication Date: 2020-05-29
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, the research on other MYB transcr

Method used

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  • Bambusa multiplex transcription factor BmMYB83 gene and application thereof
  • Bambusa multiplex transcription factor BmMYB83 gene and application thereof
  • Bambusa multiplex transcription factor BmMYB83 gene and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Acquisition of gene encoding transcription factor of filial piety bamboo BmMYB83

[0026] 1. RNA extraction

[0027] The extraction material is bamboo shoots of filial piety bamboo. The RNA was extracted using the Yuanping Hao RNAPure Ultra-Pure Rapid Extraction Kit, extracted according to the operating instructions, and stored at -80°C for later use

[0028] 2. cDNA first-strand synthesis and reverse transcription PCR

[0029] The total RNA was reverse-transcribed into cDNA using the Whole Type Gold (Beijing) one-step cDNA synthesis kit, following the operating instructions. Reaction system and reaction conditions are: 2 μg prepared total RNA, Anchored Oligo(dT) 18 Primer1μL, 10μL of 2×TS Reaction Mix, 1μL of TransScript RT / RI Enzyme Mix, 1μL of gDNARemover, add RNase-free Water to make up to 20μL, mix gently and centrifuge. Reverse transcription at 42°C for 30 minutes in a PCR instrument, 5s at 85°C; cool on ice; store at -20°C after centrifugation.

[...

Embodiment 2

[0033] Example 2: Functional Analysis of BmMYB83 Transcription Factor in Filial Bamboo Bamboo

[0034] 1. Sequence comparison analysis

[0035] In order to analyze the genetic relationship between BmMYB83 and MYB transcription factors of other species, a phylogenetic tree of BmMYB83 and MYB transcription factors of other species was established using MEGA7. result( figure 2 ) indicated that BmMYB83 had the closest relationship with OsMYB83 and OsMYB46 in the model plant rice, and belonged to the same cluster.

[0036] The homology of BmMYB83 with closely related MYB transcription factors in other species was analyzed by DNAMAN. result( image 3 ) showed that BmMYB83 had a high homology with OsMYB83 in rice, and the sequence similarity was 58.84%. The sequence similarities with rice OsMYB46 and Arabidopsis AtMYB83 were 39.44% and 38.39%, respectively.

[0037] 2. Rice transgenic analysis

[0038] 2.1 Vector construction

[0039] The correctly sequenced PCR product was l...

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Abstract

The invention discloses a bambusa multiplex transcription factor BmMYB83 gene and application thereof and belongs to the technical field of plant gene engineering. The transcription factor BmMYB83 disclosed by the invention belongs to an MYB transcription factor family, is derived from bambusa multiplex and has a nucleotide sequence shown in SEQ ID NO.1, and the amino acid sequence of a protein encoded by the gene is shown in SEQ ID NO.2. Through gene cloning expression and comparison analysis, the evolutionary status of an encoding gene system of the bambusa multiplex transcription factor BmMYB83 is revealed, a method for using the gene is also provided, plant type variation, such as planting dwarfing, leaf curling and number increase of scapes, of arabidopsis plants caused through over expression of the gene, and root lengths of rice can be also reduced. The gene disclosed by the invention plays a significant role in plant property improvement.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering, and more specifically relates to a Bambusamultiplex transcription factor BmMYB83 gene and application thereof. Background technique [0002] MYB transcription factors, one of the largest class of transcription factors in plants, are involved in various activities during plant growth and development, including stress response (Baldoni, E. et al., International Journal of Molecular Sciences, 16, 15811-15851, 2015) , hormone synthesis (Wang et al., BMC Genomics, 16:17, 2015), signal transduction. In the process of plant cell wall secondary growth, MYB transcription factors present a hierarchical network regulation mode, and participate in the synthesis of cell wall cellulose, hemicellulose and lignin. Among them, MYB46 and MYB83 are the main switches of network regulation, which are directly regulated by two types of NAC transcriptional switch factors (Zhang et al., Frontiers in P...

Claims

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Application Information

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IPC IPC(8): C12N15/29C07K14/415C12N15/82A01H5/00A01H6/46A01H6/20
CPCC07K14/415C12N15/8261
Inventor 魏强郭琳丁雨龙
Owner NANJING FORESTRY UNIV
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