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Molecular marker auxiliary selection method for tomato yellow leaf curl viral disease and knot nematode

A molecular marker-assisted, yellow leaf curl virus technology is applied in the field of molecular marker-assisted selection for simultaneous detection of tomato anti-Ty-1 and root-knot nematode genes, which can solve the problems of low breeding efficiency, heavy workload, and high cost. The effect of simplifying experiment cost, rapid detection and analysis, and reducing workload

Inactive Publication Date: 2011-01-19
NORTHWEST A & F UNIV
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Problems solved by technology

[0007] The purpose of the present invention is to provide a method for early simultaneous identification of tomato resistance to yellowing, aiming at the limitations of heavy workload, long cycle and other conditions in the screening process of tomato conventional disease-resistant breeding progeny, as well as the defects of low breeding efficiency and high cost. Molecular marker method for genotypes of leaf curl virus disease and root-knot nematode disease, so as to be used in molecular marker-assisted breeding

Method used

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  • Molecular marker auxiliary selection method for tomato yellow leaf curl viral disease and knot nematode
  • Molecular marker auxiliary selection method for tomato yellow leaf curl viral disease and knot nematode
  • Molecular marker auxiliary selection method for tomato yellow leaf curl viral disease and knot nematode

Examples

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Embodiment

[0024] Embodiment: Utilize multiple CAPs labeling technique to the partial selfing F of 7 tomato hybrid first generation, E13 and A10 2 Electrophoretic detection of separated generation materials

[0025] The specific implementation steps of this example are:

[0026] 1. Materials and methods:

[0027] B08, 109, 314, 198, 288 are the first generation tomato hybrids. Among them, B08, 109, 314, and 198 are varieties susceptible to tomato yellow leaf curl virus disease, and 288 is a variety resistant to tomato yellow leaf curl virus disease. E13: The first generation of excellent tomato hybrids, introduced from the Netherlands, this strain has red fruits, is resistant to storage and transportation, and is resistant to tomato yellow leaf curl virus. A10: The first generation of tomato hybrids, introduced from South Korea. This variety has red fruits, is resistant to storage and transportation, and is susceptible to tomato yellow leaf curl virus. Bagging selfing on E13 and A10...

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Abstract

The invention discloses a molecular marker auxiliary selection method for tomato yellow leaf curl viral disease and knot nematode. The method is characterized by comprising the following steps of: (1) extracting DNA from a tomato material F1 and a selfed separation offspring F2 individual; (2) performing multiple PCR amplification on the tomato material F1 and the selfed separation offspring F2; and (3) performing resistance evaluation on the breeding separation generation material according to the detection result. The multiple PCR amplification method can amplify Ty-1 genes and Mi genes in the same reaction system at the same time, so the method greatly simplifies experiment operation and experiment expense, is suitable for identifying and analyzing a large amount of sample material, greatly shortens the time and reduces the workload.

Description

technical field [0001] The invention relates to the technical field of crop molecular markers, in particular to a molecular marker-assisted selection method for simultaneously detecting tomato anti-Ty-1 and root-knot nematode genes. Background technique [0002] Tomato is a worldwide crop with wide adaptability and high yield. Tomato yellow leaf curl virus (Tomato yellow leaf curl virus, TYLCV) belongs to the Begamovirus genus of Geminiviridae, and is mainly transmitted by Bemisia tabaci. In recent years, due to global warming and the unprecedented expansion of Bemisia tabaci, the disease has broken out in more than 40 countries and has become more serious year by year. It has become an important limiting factor in tomato production in many parts of the world. Since cultivated tomatoes are not resistant to tomato yellow leaf curl disease (TYLCD), a large number of studies have focused on screening wild relatives in order to obtain breeding parents. If molecular markers are...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 陈书霞房玉林程智慧孟焕文杜俊娜崔鸿文
Owner NORTHWEST A & F UNIV
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