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Application of phytosulfokine-alpha in improving plant yellow leaf curl virus resistance

A technology of yellow leaf curl virus and phytosulfopeptine, which is applied in the application field of phytosulfopeptine-α in improving the resistance of plant yellow leaf curl virus disease, can solve the problems of less research and application, and achieve a prolonged Validity period, reduced dosage, enhanced resistance effect

Active Publication Date: 2016-08-31
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the research and application of biological agents for the prevention and treatment of TYLCV are still relatively few

Method used

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  • Application of phytosulfokine-alpha in improving plant yellow leaf curl virus resistance
  • Application of phytosulfokine-alpha in improving plant yellow leaf curl virus resistance
  • Application of phytosulfokine-alpha in improving plant yellow leaf curl virus resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] 1. Preparation of preparations

[0046] Take 2.54g of sulfopeptine-α (PSKα) (purchased from French NeoMPS company); slowly add 294.0mL of water, and mix the mixed solution evenly, and finally add 6.0mL of organosilicon solution to the mixed solution, stir evenly , to obtain the preparation.

[0047] 2. Application and testing of preparations

[0048] Take 100 ml of the above preparation, add 10 liters of water, mix well, and spray evenly on the surface of tomato leaves with four leaves and one heart at the seedling age in the evening until the leaves are wet, and continue spraying for 2 days. control.

[0049] The above-mentioned treated plants were picked with sterilized bamboo sticks to pick up the invasive clones of Agrobacterium and inoculated with tomato yellow leaf curl virus by acupuncture at the stalk of the first to second true leaf of tomato. All the above-treated plants were placed at 28°C, 70% humidity, 12h light / 12h dark, and the light intensity was 400μ...

Embodiment 2

[0054] 1. Preparation of preparations

[0055] Take 2.03g of sulfopeptine-α (PSKα) (purchased from NeoMPS, France); slowly add 292.5mL of water, and mix the mixed solution evenly, and finally add 7.5mL of organosilicon solution to the mixed solution, stir evenly , to obtain the preparation. 2. Application and testing of preparations

[0056] Take 100 ml of the above preparation, add 10 liters of water, mix well, and spray evenly on the surface of tomato leaves with three leaves and one heart at the seedling age in the evening until the leaves are wet, and continue spraying for 2 days. control.

[0057] The above-mentioned treated plants were picked with sterilized bamboo sticks to pick up the invasive clones of Agrobacterium and inoculated with tomato yellow leaf curl virus by acupuncture at the stalk of the first to second true leaf of tomato. All the plants treated above were placed at 32°C, 70% humidity, 12h light / 12h dark, and the light intensity was 400μmol m -2 the s...

Embodiment 3

[0062] 1. Preparation of preparations

[0063] Take 3.05g of sulfopeptine-α (PSKα) (purchased from French NeoMPS company); slowly add 291.0mL of water, and mix the mixed solution evenly, and finally add 9.0mL of organosilicon solution to the mixed solution, stir evenly , to obtain the preparation.

[0064] 2. Application and testing of preparations

[0065] Select two adjacent plastic greenhouses with the same cultivation technology and management. In the high temperature and less rainy season in early autumn, use the above preparation for the treatment shed. Take 200 ml of the preparation, add 20 liters of clear water, mix well, and divide it into two parts in the morning and evening. Spray evenly on the surface of tomato leaves with a seedling age of seven leaves and one heart until the leaves are wet, and continue spraying for 2 days; while the control shed only sprays the same volume of water.

[0066] After 20 days, the disease incidence of 60 tomato plants in the treat...

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Abstract

The invention discloses an application of phytosulfokine-alpha in improving plant yellow leaf curl virus resistance and an application of the phytosulfokine-alpha in preparing preparations capable of improving plant yellow leaf curl virus resistance. According to the preparations prepared by the active ingredient, namely the phytosulfokine-alpha, signal paths of auxin in plants are induced to improve the resistance to the yellow leaf curl virus remarkably, so that economic loss caused by the yellow leaf curl virus to plants is decreased. The way of applying the preparations to prevent the plant yellow leaf curl virus is simple and easy and low in cost, capable of delaying and suppressing growth of the plant yellow leaf curl virus on leaves and spreading of diseases remarkably and improving the plant yellow leaf curl virus resistance greatly.

Description

technical field [0001] The invention relates to the technical field of phytochemical protection, in particular to the application of phytosulfopeptine-α in improving the resistance of plants to yellow leaf curl virus disease. Background technique [0002] The cultivated area and total output of tomato in my country rank first in the world, and the demand and planted area are increasing year by year. Viral diseases seriously threaten the production safety of tomatoes. Among them, the viral diseases caused by tomato yellow leafcurl virus (Tomato yellow leafcurl virus, TYLCV) were first discovered in Israel in the late 1930s and introduced into my country in the 1990s. It has become a devastating disease that seriously endangers tomato production. [0003] The main vector of yellow leaf curl virus is Bemisia tabaci, which can infect crops such as Solanaceae, Leguminosae and Brassicaceae. Symptoms of the disease on tomatoes are mainly: initial growth retardation or stagnation, ...

Claims

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Application Information

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IPC IPC(8): A01N41/04A01P1/00A01P21/00
CPCA01N41/04
Inventor 师恺张欢史军营宰文珊齐振宇喻景权
Owner ZHEJIANG UNIV
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