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31 results about "Dot elisa" patented technology
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The dot enzyme-linked immunosorbent assay (Dot-ELISA) is a highly versatile solid-phase immunoassay for antibody or antigen detection.
MAb-based Dot-ELISA method and assay kit for the detection of viruses
Owner:PENN STATE RES FOUND
Dot enzyme-linked immunosorbent assay (ELISA) method for detecting bemisia tabaci carrying tomato yellow leaf curl virus and application of method
ActiveCN102937652AQuick checkRealize detectionMaterial analysisDot elisaTomato yellow leaf curl virus
Owner:ZHEJIANG UNIV
Hybridoma cell strain secreting monoclonal antibody resistant to Candidatus Liberibacter asiaticus pathogens and application of monoclonal antibody
ActiveCN109897829AAccurate detectionSensitive detectionImmunoglobulins against bacteriaMicroorganism based processesAntibody typesBALB/c
The invention discloses a hybridoma cell strain secreting a monoclonal antibody resistant to Candidatus Liberibacter asiaticus pathogens and an application of the monoclonal antibody. A Candidatus Liberibacter asiaticus vein crude extract is taken as an antigen to immunize a BALB / c mouse, one hybridoma cell strain 11H9 capable of secreting the monoclonal antibody resistant to Candidatus Liberibacter asiaticus pathogens is obtained by cell fusion, screening and cloning, and the preservation number of the hybridoma cell strain is CGMCC No.17285. Indirect ELISA valence of monoclonal antibody ascites secreted by the cell strain reaches 10<-7>, the type and the subclass of the antibody are IgG1 and kappa light chains, and the monoclonal antibody has a specific reaction with Candidatus Liberibacter asiaticus pathogen protein of 56 kDa in sick leaves and avoids immunoreaction with healthy leaves. ACP-ELISA, dot-ELISA and Tissue blot-ELISA detection methods for detecting the Candidatus Liberibacter asiaticus pathogens are established on the basis of the monoclonal antibody11H9, wherein sensitivity of the ACP-ELISA and dot-ELISA methods for detecting the Candidatus Liberibacter asiaticus vein crude extract reaches 20480-fold dilution and 10240-fold dilution (w / v,g / mL). The material and technical support is provided for diagnosis and detection of Candidatus Liberibacter asiaticus, epidemiological analysis, sterile seedling production and scientific prevention and control through establishment of the preparation and detection methods of the monoclonal antibody resistant to the Candidatus Liberibacter asiaticus pathogens.
Owner:ZHEJIANG UNIV
Mab-based dot-elisa method and assay kit for the detection of viruses
InactiveUS20060105328A1Rapid and less-costly methodMicrobiological testing/measurementEnzymologyAnimal virusAntigen binding
A monoclonal antibody-based Dot-ELISA assay for the rapid detection of animal viruses such as avian influenza virus. The assay includes applying a specimen suspected of containing an animal virus on a porous membrane and treating the specimen with a solution of citric acid or lactic acid and a solution containing a mucolytic agent and a detergent. The treated specimen is then contacted with a primary monoclonal antibody for detecting the virus. If present, the primary moncolonal antibody bind with an antigen of the animal virus specimen. The specimen is contacted with an anti-monoclonal antibody conjugate (secondary antibody) and incubated to facilitate binding of the antigen-bound monoclonal antibody to the conjugate. The bound conjugate and antigen-bound monoclonal antibody is contacted with a coloring reagent to allow visual detection of the presence of the animal virus in the specimen.
Owner:PENN STATE RES FOUND
Hybridoma cell strain secreting potato-virus-Y-resistant monoclonal antibodies and monoclonal antibody application thereof
ActiveCN105543176ASensitive detectionAccurate detectionImmunoglobulins against virusesTissue cultureBALB/cAntibody types
Owner:ZHEJIANG UNIV
Rapid detection method for chili (sweet) pepper root rot diseases
The invention provides a rapid detection method for chili (sweet) pepper root rot diseases, which belongs to the field of biotechnology. The invention relates to the rapid detection method for chili (sweet) pepper root rot diseases, which is characterized in that the method applies an improved novel technique of ELISA, namely a Dot-ELISA technique, is low in consumption of reagents, simple and fast to operate, free from need for special equipment, applicable to basic units, long in preservation period of antigen membrane; 20 percent of antigen membrane can be preserved for half an year without affecting the activity of the antigen membrane; the antigen membrane can be mailed for on-site epidemiological investigation; detection result can be kept for a long time to provide convenience for reexamination; and the method is suitable for on-site detection and the rapid prediction of the epidemic occurrence of diseases. The invention also relates to detection programs, working conditions, detectable measurement, medicaments and appliances used for detection.
Owner:INST OF VEGETABLE & FLOWERS CHINESE ACAD OF AGRI SCI
Hybridoma cell strain secreting citrus yellow vein clearing virus-resistant monoclonal antibodies and monoclonal antibody application thereof
ActiveCN105543177AAccurate detectionSensitive detectionImmunoglobulins against virusesMicroorganism based processesBALB/cAntibody types
The invention discloses a hybridoma cell strain secreting citrus yellow vein clearing viru(CYVCV)-resistant monoclonal antibodies and monoclonal antibody application thereof. Coat protein of prokaryotic-expression CYVCV serves as antigens to immune BALB / c mice, cell fusion, screening and cloning are performed, one hybridoma cell strain 18H5 which is stable in passage and can secrete CYVCV-resistant monoclonal antibodies is obtained, and the preservation number of the hybridoma cell strain 18H5 is CGMCC No. 12003. The ascites indirect ELISA titer of the monoclonal antibodies secreted by the cell strain reaches 10<-7>, according to the antibody type and the subclass, the monoclonal antibodies are composed of IgG1 and kappa light chains, and the monoclonal antibodies and CYVCV coat protein subunits of 32 kD have a specific reaction. The 18H5 monoclonal antibodies are used for establishing TAS-ELISA, dot-ELISA and Tissue blot-ELISA detecting methods of CYVCV in citrus trees, wherein the diseased leaf detecting sensitivity of the TAS-ELISA method and the diseased leaf detecting sensitivity of the dot-ELISA method reach 1:2560 and 1:20480 fold dilution (w / v, g / mL) respectively. Preparation of the CYVCV-resistant monoclonal antibodies and establishment of the detection methods of the CYVCV-resistant monoclonal antibodies provide matter and technologic support for detection and diagnosis, epidemiological analysis and scientific prevention and control of citrus virus diseases.
Owner:ZHEJIANG UNIV
Hybridoma cell strain capable of secreting anti-Southern-bean-mosaic-virus monoclonal antibody and application of monoclonal antibody thereof
ActiveCN105671001AAccurate detectionSensitive detectionImmunoglobulins against virusesTissue cultureBALB/cAntibody types
The invention discloses a hybridoma cell strain capable of secreting an anti-Southern-bean-mosaic-virus monoclonal antibody and an application of the monoclonal antibody thereof. Purified Southern bean mosaic virus (SBMV) particles serve as antigen immunization BALB / c mice, and are subjected to cell fusion, cell screening and cell cloning, the hybridoma cell strain 19H9 capable of conducting stable passage and secreting the anti-SBMV monoclonal antibody is obtained, and the collection number is CGMCC No.12004. The ascites indirect ELISA titer of the monoclonal antibody secreted by the hybridoma cell strain is 10<-7>, and the type and the subgenera of the antibody are IgG1 light chains and kappa light chains. The monoclonal antibody and SBMV are subjected to a specific reaction. An ACP-ELISA method for detecting the SBMV in leguminous plants and a dot-ELISA method for detecting the SBMV in leguminous plants are built with the monoclonal antibody 19H9, and the diseased leaf detection sensitivity of the ACP-ELISA method and the dot-ELISA method is 1:81,920 w / v and 1:5,120 g / ml. The hybridoma cell strain 19H9, the obtained monoclonal antibody and the built serological detecting method provide substance supports and technical supports for diagnosing, detecting and scientific preventing and controlling of the virus disease.
Owner:ZHEJIANG UNIV +1
A diagnostic kit for detecting paragonimiasis and its preparation method
InactiveCN102279259AFacilitate early diagnosisAids in prognosisMaterial analysis by observing effect on chemical indicatorDiseaseNitrocellulose
The invention provides a diagnostic kit for detecting Paragonimiasis, which contains a rabbit polyclonal antibody against the soluble antigen of Paragonia wescheri adult worm, an enzyme-labeled rabbit polyclonal antibody against the soluble antigen of Paragonimus westermani adult worm, Nitrocellulose membrane, bovine serum albumin, PBST washing solution, substrate chromogenic solution, positive control sample, negative control sample and polyethylene reaction plate. The invention also provides a preparation method of a diagnostic kit for detecting paragonimiasis. The invention adopts the double-antibody sandwich method Dot-ELISA to detect the circulating antigen of Paragonimus westermani, has high sensitivity and strong specificity, and can realize rapid diagnosis and curative effect assessment of Paragonimiasis.
Owner:STATION OF VIRUS PREVENTION & CONTROL CHINA DISEASES PREVENTION & CONTROL CENT
Hybridoma cell strain secreting monoclonal antibody resistant to tomato black ring nepovirus and application of monoclonal antibody of hybridoma cell strain
ActiveCN105624121AAccurate detectionSensitive detectionMicroorganism based processesImmunoglobulins against virusesDiseaseAntibody types
The invention discloses a hybridoma cell strain secreting a monoclonal antibody resistant to tomato black ring nepovirus and application of the monoclonal antibody of the hybridoma cell strain.Purified tomato black ring nepovirus (TBRV) particles serve as antigen to immunize a BALB / c mouse, cell fusion, screening and cloning are conducted, the hybridoma cell strain 12H3 which can stably subculture and secrete the TBRV-resistant monoclonal antibody is obtained, and the preservation No.of the hybridoma cell strain is CGMCC No.12005.The titer of ascites indirect ELISA of the monoclonal antibody secreted by the hybridoma cell strain reaches 10<-7>, and the type and subclass of the antibody is an IgG1,kappa light chain.The monoclonal antibody has a specific immunoreaction with 50kDa TBRV coat protein.An ACP-ELISA method and a dot-ELISA method for detecting TBRV in tomatoes are established through the 12H3 monoclonal antibody, and sensitivity of the ACP-ELISA method and sensitivity of the dot-ELISA method for detecting leaves attacked by disease reach 1: 163, 840 and 1: 5, 120 times dilution (w / v, g / mL) respectively.Through obtaining of the hybridoma cell strain 12H3 and the monoclonal antibody and establishment of the serological test methods, material support and technical support are provided for detection, quanrantine and scientific prevention and control for the virus.
Owner:ZHEJIANG UNIV +1
Method for detecting dot-ELISA of principally outer membrane protein of vibrio vulnficus gene
Disclosed is dot-ELISA detection method of main outer-membrane protein of vibrio vulnficus. Cultured vibrio vulnficus is crashed (power 200w, beyond 5s stopping 5s) through ultrasonic, supernatant is centrifugally collected. Spotting 1.0 mu l is on a polyvinylidene fluoride film (PVDF film), after the spotting PVDF film is closed by 2% BSA (bovine serum albumin) under 37 DEG C, respectively reacted with rabbit anti vibrio vulnficus serum and goat anti-rabbit IgG whole serum marked by HRP (horseradish peroxide), finally reacted with substrate solution, macroscopic brown yellow round sedimentation is formed on the PVDF film, namely masculine result. The inventive method has detection sensitivity 15 ng / ml for main outer-membrane protein of vibrio vulnficus, is 3-5 times higher than enzyme linked immunosorbent assay (ELISA), the result is obtained after 2-3 hours, therefore vibrio vulnficus in the marine environment is detected fast and sensitively.
Owner:å¼ æŒ¯å†¬
Application of hybridoma cell line for secreting monoclonal antibody resisting rice strip mosaic virus (RSMV) and monoclonal body of hybridoma cell line
ActiveCN108165533AAccurate detectionSensitive detectionImmunoglobulins against virusesMicroorganism based processesAntibody typesBALB/c
The invention discloses application of a hybridoma cell line for secreting a monoclonal antibody resisting rice strip mosaic virus (RSMV) and the monoclonal antibody of the hybridoma cell line. The purified RSMV is an antigen immune BALB / c mouse; a hybridoma cell line 1D4 which can stably pass and secrete the monoclonal antibody resisting the RSMV is obtained by cell fusion, screening and colonizing, and the preservation number of the hybridoma cell line 1D4 is CGMCC (China General Microbiological Culture) No.14898. The ascites indirect ELISA (Enzyme Lined Immunosorbent Assay) titer of the monoclonal antibody secreted by the hybridoma cell reaches 10<-10>, and the type and subclass of the antibody are IgG1 and kappa chains. The monoclonal antibody has specific reaction with N protein of the RSMV. An ACP-ELISA method and a dot-ELISA method for detecting the RSMV are established by taking the 1D4 as a core; the sensitivities of the two methods for detecting disease leaves respectively reach the dilution of 1 to 2621440 and the dilution of 1 to 40960 (w / v, g / mL). The obtaining of the hybridoma cell line and the monoclonal antibody thereof and establishment of a serological testing method provide material and technical support for detection and diagnosis and scientific prevention and control of virus disease.
Owner:ZHEJIANG UNIV
Recombined prokaryotic expression detection antigen for detecting local trichina nematode antibody and producing method thereof
InactiveCN1811431AImprove stabilityHigh sensitivityMicrobiological testing/measurementBiological testingEscherichia coliNematode
The present invention provides a recombinant prokaryotic expression protein antigen for detecting autochthonous trichinella spiralis antibody and its preparation method. Said product can be used as detection antigen of indirect-ELISA and Dot-ELISA method for detecting autochthonous trichinella spiralis antibody, also can be used as gene engineering subunit vaccine for preventing autochthonous trichinella spiralis infection. The preparation process of said product includes the following steps: (1) designing specific primer for amplifying TNPG gene; (2), utilizing PCR amplification to obtain TNPG gene segment; (3), cloning TNPG gene and sequencing, and making identification; (4), directionally subcloning TNPG gene to pET-3a prokaryotic expression vector; (5), induced expression of TNPG gene in colibacillus; and (6), affinity chromatography purification of TNPG gene expression protein.
Owner:NORTHEAST AGRICULTURAL UNIVERSITY
A kind of canine single-chain antibody and its construction method and application
InactiveCN104017080BImprove immunityHave diversityPeptide librariesImmunoglobulins against blood group antigensSingle-Chain AntibodiesBlood typing
The invention relates to the field of biotechnology, and specifically discloses a canine single-chain antibody and its construction method and application. The invention successfully amplifies the canine heavy chain variable region VH and light chain variable region VL genes by designing degenerate primers for the variable region of the canine antibody. On this basis, the canine single-chain antibody scFv library was successfully constructed using phage display technology. The constructed scFv single-chain antibody library has diversity and large enough library capacity. The present invention uses the dog DEA1.1 blood group antigen to screen the scFv single-chain antibody library to obtain 3 single-chain antibodies capable of binding to the dog DEA1.1 blood group antigen by Dot-ELISA method. Among them, Clone16 has strong binding properties. The pET‑32a‑Clone16 recombinant protein was constructed based on the Clone16 gene for prokaryotic expression, and the purified antibody had binding activity. It provides a solid foundation for the preparation of canine DEA1.1 blood group identification reagent.
Owner:SOUTH CHINA AGRI UNIV
Hybridoma cell strain secreting PPV (plum pox virus)-resistant monoclonal antibody and application of monoclonal antibody
ActiveCN109897828ASensitive detectionAccurate detectionImmunoglobulins against virusesMicroorganism based processesBALB/cAntibody types
The invention discloses a hybridoma cell strain secreting a PPV (plum pox virus)-resistant monoclonal antibody and an application of the monoclonal antibody. Prokaryotic-expression PPV coat protein istaken as an antigen to immunize a BALB / c mouse, one hybridoma cell strain 4A8 capable of secreting the PPV-resistant monoclonal antibody is obtained by cell fusion, screening and cloning, and the preservation number of the hybridoma cell strain is CGMCC No.17283. Indirect ELISA valence of monoclonal antibody ascites secreted by the cell strain reaches 10<-7>, the type and the subclass of the antibody are IgG1 and kappa light chains, and the monoclonal antibody has a specific reaction with coat protein subunit of PPV. TAS-ELISA and dot-ELISA detection methods for detecting the PPV in plum trees are established on the basis of the monoclonal antibody 4A8, wherein sensitivity of the TAS-ELISA and dot-ELISA methods for detecting sick leaves reaches 20480-fold dilution and 5120-fold dilution (w / v,g / mL). The material and technical support is provided for diagnosis and detection of PPV disease in an orchard, import and export port inspection and quarantine as well as scientific prevention and control through establishment of the preparation and detection methods of the PPV monoclonal antibody.
Owner:ZHEJIANG UNIV
Hybridoma Cell Line Secreting Monoclonal Antibody Against Southern Bean Mosaic Virus and Application of Monoclonal Antibody
ActiveCN105671001BAccurate detectionSensitive detectionImmunoglobulins against virusesTissue cultureBALB/cAntibody types
The invention discloses a hybridoma cell strain capable of secreting an anti-Southern-bean-mosaic-virus monoclonal antibody and an application of the monoclonal antibody thereof. Purified Southern bean mosaic virus (SBMV) particles serve as antigen immunization BALB / c mice, and are subjected to cell fusion, cell screening and cell cloning, the hybridoma cell strain 19H9 capable of conducting stable passage and secreting the anti-SBMV monoclonal antibody is obtained, and the collection number is CGMCC No.12004. The ascites indirect ELISA titer of the monoclonal antibody secreted by the hybridoma cell strain is 10<-7>, and the type and the subgenera of the antibody are IgG1 light chains and kappa light chains. The monoclonal antibody and SBMV are subjected to a specific reaction. An ACP-ELISA method for detecting the SBMV in leguminous plants and a dot-ELISA method for detecting the SBMV in leguminous plants are built with the monoclonal antibody 19H9, and the diseased leaf detection sensitivity of the ACP-ELISA method and the dot-ELISA method is 1:81,920 w / v and 1:5,120 g / ml. The hybridoma cell strain 19H9, the obtained monoclonal antibody and the built serological detecting method provide substance supports and technical supports for diagnosing, detecting and scientific preventing and controlling of the virus disease.
Owner:ZHEJIANG UNIV +1
Hybridoma cell strain capable of secreting BYDV (barley yellow dwarf virus) PAV strain monoclonal antibody and application of monoclonal antibody of hybridoma cell strain
ActiveCN106636008AAccurate detectionSensitive detectionImmunoglobulins against virusesTissue cultureAntibody typesBALB/c
The invention discloses a hybridoma cell strain capable of secreting a BYDV (barley yellow dwarf virus) PAV strain monoclonal antibody and an application of the monoclonal antibody of the hybridoma cell strain. Purified BYDV PAV strain virions are used as an antigen to immunize a BALB / c mouse, one hybridoma cell strain 24G4 capable of passaging steadily and secreting the BYDV PAV strain monoclonal antibody is obtained through cell fusion, screening and cloning, and the preservation number of the hybridoma cell strain 24G4 is CGMCC No.13298. The indirect ELISA titer of monoclonal antibody ascites secreted by the hybridoma cell strain is 10<-9>, and the antibody type and subtype are IgG1 and kappa chain. ACP-ELISA and dot-ELISA methods for detecting BYDV PAV in wheat plants are established with the monoclonal antibody 24G4 as the core, and the sensitivities of the two methods for detecting infected leaves reach 1:81920 and 1:1280 fold dilution (w / v, g / mL). The hybridoma cell strain 24G4, the monoclonal antibody obtained from the same and the established serological test methods provide material and technical support for detection, diagnosis and scientific prevention and control of the viral diseases.
Owner:ZHEJIANG UNIV
Dot enzyme-linked immunosorbent assay (ELISA) method for detecting bemisia tabaci carrying tomato yellow leaf curl virus and application of method
ActiveCN102937652BQuick checkRealize detectionMaterial analysisDot elisaTomato yellow leaf curl virus
The invention discloses a dot enzyme-linked immunosorbent assay (ELISA) method for detecting bemisia tabaci carrying a tomato yellow leaf curl virus and application of the method. Field bemisia tabaci is collected, pretreated and grinded, monoclonal antibody of the tomato yellow leaf curl virus is used, the dot-ELISA method which is used for detecting virus transmission medium bemisia tabaci is established, a quick kit is developed and researched, and the tomato yellow leaf curl virus which is carried by the bemisia tabaci is detected. By the aid of the method, the virus carrying rate of the field bemisia tabaci can be surveyed in a large scale, and the field tomato yellow leaf curl disease outbreak trend is predicted. The method is quick, efficient, sensitive, special, high in flux and simple to operate. The tomato yellow leaf curl disease can be diagnosed quickly, predicted and controlled scientifically.
Owner:ZHEJIANG UNIV
Aeromonas hydrophila aerolysin Dot-ELISA detection method
InactiveCN102012429BEasy to purifyAvoid degradationSerum immunoglobulinsImmunoglobulins against bacteriaSerum igeDot elisa
Owner:NANJING AGRICULTURAL UNIVERSITY
Hybridoma cell line secreting monoclonal antibody against citrus chlorosis virus and application of monoclonal antibody
ActiveCN105543177BAccurate detectionSensitive detectionImmunoglobulins against virusesMicroorganism based processesBALB/cAntibody types
The invention discloses a hybridoma cell strain secreting citrus yellow vein clearing viru(CYVCV)-resistant monoclonal antibodies and monoclonal antibody application thereof. Coat protein of prokaryotic-expression CYVCV serves as antigens to immune BALB / c mice, cell fusion, screening and cloning are performed, one hybridoma cell strain 18H5 which is stable in passage and can secrete CYVCV-resistant monoclonal antibodies is obtained, and the preservation number of the hybridoma cell strain 18H5 is CGMCC No. 12003. The ascites indirect ELISA titer of the monoclonal antibodies secreted by the cell strain reaches 10<-7>, according to the antibody type and the subclass, the monoclonal antibodies are composed of IgG1 and kappa light chains, and the monoclonal antibodies and CYVCV coat protein subunits of 32 kD have a specific reaction. The 18H5 monoclonal antibodies are used for establishing TAS-ELISA, dot-ELISA and Tissue blot-ELISA detecting methods of CYVCV in citrus trees, wherein the diseased leaf detecting sensitivity of the TAS-ELISA method and the diseased leaf detecting sensitivity of the dot-ELISA method reach 1:2560 and 1:20480 fold dilution (w / v, g / mL) respectively. Preparation of the CYVCV-resistant monoclonal antibodies and establishment of the detection methods of the CYVCV-resistant monoclonal antibodies provide matter and technologic support for detection and diagnosis, epidemiological analysis and scientific prevention and control of citrus virus diseases.
Owner:ZHEJIANG UNIV
Hybridoma cell line secreting anti-wheat dwarf virus monoclonal antibody and its application
ActiveCN106636009BAccurate detectionSensitive detectionImmunoglobulins against virusesTissue cultureBALB/cAntibody types
The invention discloses a hybridoma cell strain capable of secreting a wheat dwarf virus resisting monoclonal antibody and a monoclonal antibody application of the strain. WDV (wheat dwarf virus) CP recombinant protein in prokaryotic expression is used as an antigen immune for BALB / c mice, the hybridoma cell strain 23F4 capable of stably going down to the future generation and secreting the WDV antibody is obtained after cell fusion, screening and cloning, and the collection number of the strain is CGMCC No. 13297. The abdominal dropsy indirect ELISA (enzyme-linked immuno sorbent assay) valence of the antibody secreted by the hybridoma cell strain reaches 10<-9>, and the antibody type and subclass are IgG1 and kappa chains. The antibody and WDV coat protein have specific reaction. The 23F4 antibody is used as a core for building ACP-ELISA and dot-ELISA methods for detecting WDV in a wheat plant, and diseased leaf detection sensitivity of the two methods reach 1:163840 and 1:5120 times of dilution (w / v, g / mL) respectively. Acquisition of the hybridoma cell strain and the antibody thereof and building of serological detection methods provide substantial and technical support for virus disease detection, diagnosis and scientific prevention and control.
Owner:ZHEJIANG UNIV
Hybridoma cell line secreting monoclonal antibody against rice tumor dwarf virus and application of monoclonal antibody
ActiveCN108486065BAccurate detectionSensitive detectionMicroorganism based processesImmunoglobulins against virusesBALB/cAntibody types
The invention discloses a use of a hybridoma cell line secreting a monoclonal antibody against rice gall dwarf viruses and a use thereof. A BALB / c mouse is immunized by a prokaryotically expressed rice gall dwarf virus (RGDV) CP recombinant protein as an antigen, and a hybridoma cell line 11B6 capable of stable passage and secretion of an anti-RGDV monoclonal antibody is obtained by cell fusion, screening and cloning and has a preservation number of CGMCC No. 14897. The indirect ELISA titer of the ascites monoclonal antibody secreted by the hybridoma cell line is 10<-8>. The antibody type andsubclass are IgG1 and kappa chains. The monoclonal antibody can react specifically with the RGDV coat protein. The ACP-ELISA and dot-ELISA methods for detecting RGDV are established through a 11B6 monoclonal antibody as the core. The sensitivity of the two methods for detecting diseased leaves is 1:327 680-fold dilution (w / v, g / mL) and 1:20 480-fold dilution (w / v, g / mL). The acquisition of the hybridoma cell line and its monoclonal antibody and the establishment of serological detection methods provide material and technical support for the detection, diagnosis and scientific prevention and control of the viral disease.
Owner:ZHEJIANG UNIV
Recombined prokaryotic expression detection antigen for detecting local trichina nematode antibody and producing method thereof
InactiveCN100445743CImprove stabilityHigh sensitivityMicrobiological testing/measurementBiological testingEscherichia coliNematode
The present invention provides a recombinant prokaryotic expression protein antigen for detecting autochthonous trichinella spiralis antibody and its preparation method. Said product can be used as detection antigen of indirect-ELISA and Dot-ELISA method for detecting autochthonous trichinella spiralis antibody, also can be used as gene engineering subunit vaccine for preventing autochthonous trichinella spiralis infection. The preparation process of said product includes the following steps: (1) designing specific primer for amplifying TNPG gene; (2), utilizing PCR amplification to obtain TNPG gene segment; (3), cloning TNPG gene and sequencing, and making identification; (4), directionally subcloning TNPG gene to pET-3a prokaryotic expression vector; (5), induced expression of TNPG gene in colibacillus; and (6), affinity chromatography purification of TNPG gene expression protein.
Owner:NORTHEAST AGRICULTURAL UNIVERSITY
Hybridoma cell strain secreting monoclonal antibody against rice ragged stunt virus and use of monoclonal antibody
ActiveCN102533665AAccurate detectionSensitive detectionImmunoglobulins against virusesTissue cultureBALB/cAntibody types
The invention discloses a hybridoma cell strain secreting a monoclonal antibody against rice ragged stunt viruses (RRSV) and use of the monoclonal antibody. Purified RRSV coat protein is used as an antigen to immunize a BALB / C mouse, and thus a hybridoma cell strain 4H5 which can be passed down steadily and can secrete monoclonal antibody against RRSV is obtained, wherein the collection number ofthe hybridoma cell strain 4H5 is CGMCCNo.5536. The result of Westernblot detection indicates the monoclonal antibody secreted by the hybridoma cell strain 4H5 reacts with the RRSV coat protein specifically. The indirect enzyme-linked immuno sorbent assay(ELSA) titer of the hybridoma cell strain 4H5 monoclonal antibody ascitic fluid reaches 10<-7>, the type and subtype of the antibody is IgG1 and kappa chain. According to a dot-ELISA detection method established by using the 4H5 monoclonal antibody for detecting brown rice plant hopper and RRSV in rice, when single-head brown rice plant hopperis diluted to 800mu L and affected leaves is diluted according to a weight-volume (g / mL) ratio of 1:320, viruses can still be detected. The established dot-ELISA method can detect brown rice plant hopper and RRSV in rice accurately, specifically and sensitively. The preparation of the monoclonal antibody against RRSV and the establishment of the detection method thereof provide technical and material supports for diagnosing, predicting and forecasting and scientifically preventing and controlling rice virus diseases.
Owner:ZHEJIANG UNIV
Hybridoma cell line secreting monoclonal antibody against watermelon mosaic virus and its application
ActiveCN104513811BAccurate detectionSensitive detectionImmunoglobulins against virusesMicroorganism based processesBALB/cAntibody types
Owner:ZHEJIANG UNIV
Sorghum mosaic virus indirect ELISA detection method and its kit and application
ActiveCN107290532BQuick checkRapid high-throughput detectionMaterial analysisDot elisaPolyclonal antibodies
Owner:GUANGXI UNIV
Hybridoma cell strain capable of secreting anti-apple stem grooving virus (ASGV) monoclonal antibody, antibody secreted by hybridoma cell strain, application of monoclonal antibody and kit prepared from monoclonal antibody
InactiveCN104232591AAccurate detectionSensitive detectionMicroorganism based processesImmunoglobulins against virusesPEARDot elisa
The invention relates to a hybridoma cell strain capable of secreting an anti-apple stem grooving virus (ASGV) monoclonal antibody and application of the monoclonal antibody. The hybridoma cell strain is collected in China center for type culture collection (CCTCC), along with a collection name of hybridoma cell strain ASGV and a collection number of CCTCC NO:C201485. The hybridoma cell strain has the benefits as follows: firstly, the provided hybridoma cell strain ASGV is capable of secreting the anti-ASGV specific monoclonal antibody, and immunological methods of TAS-ELISA (three-antibody sandwich-enzyme-linked immuno sorbent assay), dot-ELISA and the like built by taking the monoclonal antibody as a core and a kit built by the methods are capable of highly specifically, accurately and sensitively detecting the ASGV; secondly, the prepared monoclonal antibody can be used for detecting the ASGV without expensive instruments and reagents; thirdly, the prepared monoclonal antibody can be used for effectively detecting the ASGV in ASGV hosts of imported nursery stocks and field apples, pears or the like.
Owner:INSPECTION & QUARANTINE TECH CENT SHANDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
Hybridoma cell line secreting monoclonal antibody against rice stripe mosaic virus and its application
ActiveCN108165533BAccurate detectionSensitive detectionMicroorganism based processesImmunoglobulins against virusesBALB/cAntibody types
The invention discloses application of a hybridoma cell line for secreting a monoclonal antibody resisting rice strip mosaic virus (RSMV) and the monoclonal antibody of the hybridoma cell line. The purified RSMV is an antigen immune BALB / c mouse; a hybridoma cell line 1D4 which can stably pass and secrete the monoclonal antibody resisting the RSMV is obtained by cell fusion, screening and colonizing, and the preservation number of the hybridoma cell line 1D4 is CGMCC (China General Microbiological Culture) No.14898. The ascites indirect ELISA (Enzyme Lined Immunosorbent Assay) titer of the monoclonal antibody secreted by the hybridoma cell reaches 10<-10>, and the type and subclass of the antibody are IgG1 and kappa chains. The monoclonal antibody has specific reaction with N protein of the RSMV. An ACP-ELISA method and a dot-ELISA method for detecting the RSMV are established by taking the 1D4 as a core; the sensitivities of the two methods for detecting disease leaves respectively reach the dilution of 1 to 2621440 and the dilution of 1 to 40960 (w / v, g / mL). The obtaining of the hybridoma cell line and the monoclonal antibody thereof and establishment of a serological testing method provide material and technical support for detection and diagnosis and scientific prevention and control of virus disease.
Owner:ZHEJIANG UNIV
Hybridoma strain capable of secreting monoclonal antibodies for preventing citrus tristeza virus and monoclonal antibody applications thereof
ActiveCN104403999AAccurate detectionSensitive detectionImmunoglobulins against virusesMicroorganism based processesBALB/cAntibody types
The invention discloses a hybridoma strain capable of secreting monoclonal antibodies for preventing citrus tristeza virus and monoclonal antibody applications thereof. The coat protein gene of citrus tristeza virus (CTV) is cloned, the CTV coat protein is expressed by utilizing a prokaryotic expression system, then the purified coat protein is taken as an antigen to immunize a BALB / c mouse, through the steps of cell fusion, screening, and cloning, a hybridoma strain 14H11, which can stably pass the characteristics to the next generation and secret monoclonal antibodies for preventing CTV, is obtained, and the preservation number of the hybridoma strain is CGMCC No.9335. The ascites indirect ELISA titer of the 14H11 monoclonal antibody can reach 10-8, and the antibody type and subtype are IgG1, kappa chain. The established dot-ELISA method can precisely, specifically, and sensitively detect the CTV from the orange species samples. The preparation of CTV monoclonal antibody and establishment of a detection method utilizing the monoclonal antibody provide technological and material support for the diagnosis, detection, and scientific prevention and control on CTV.
Owner:ZHEJIANG UNIV +1
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