36 results about "Citrus tristeza virus" patented technology

The invention relates to a multi-RT-PCR (reverse transcription-polymerase chain reaction) method for simultaneously detecting citrus yellow vein clearing viruses and citrus tristeza viruses. The multi-RT-PCR method has the advantages that Mg<2+> and dNTP (diethyl-nitrophenyl thiophosphate) concentration, primer concentration and annealing temperatures which can affect multi-PCR are adjusted and optimized by the aid of selected specific primers of conserved sequences and reference genes of coat proteins of two types of pathogens of the CYVCV (citrus yellow vein clearing viruses) and the CTV (citrus tristeza viruses), the one-step multi-RT-PCR method for simultaneously detecting the CYVCV and the CTV can be created, and the sensitivity of the multi-RT-PCR method is consistent with single RT-PCR detection sensitivity; as shown by results of detection on 33 field samples by the aid of the multi-RT-PCR method, the CYVCV and CTV infection rates are 54.5% and 66.7% respectively, and 36.4% of samples are infected in a mixed manner; the multi-RT-PCR method can be used for simultaneously quickly detecting the CYVCV and the CTV in large quantities of field samples and has important significance on improving efficient and accurate detection and timely and effective prevention and control on the citrus yellow vein clearing viruses and the citrus tristeza viruses.

The invention discloses a hybridoma strain capable of secreting monoclonal antibodies for preventing citrus tristeza virus and monoclonal antibody applications thereof. The coat protein gene of citrus tristeza virus (CTV) is cloned, the CTV coat protein is expressed by utilizing a prokaryotic expression system, then the purified coat protein is taken as an antigen to immunize a BALB / c mouse, through the steps of cell fusion, screening, and cloning, a hybridoma strain 14H11, which can stably pass the characteristics to the next generation and secret monoclonal antibodies for preventing CTV, is obtained, and the preservation number of the hybridoma strain is CGMCC No.9335. The ascites indirect ELISA titer of the 14H11 monoclonal antibody can reach 10-8, and the antibody type and subtype are IgG1, kappa chain. The established dot-ELISA method can precisely, specifically, and sensitively detect the CTV from the orange species samples. The preparation of CTV monoclonal antibody and establishment of a detection method utilizing the monoclonal antibody provide technological and material support for the diagnosis, detection, and scientific prevention and control on CTV.