Method for rapidly detecting citrus tristeza virus by electrospray ionization mass spectrometry

Abstract

The invention provides a method for rapidly detecting citrus tristeza virus by electrospray ionization mass spectrometry. The electrospray ionization mass spectrometry is used to determine the changeof metabolites in citrus leaves, and the infected citrus nursery stocks at the early stage of the incubation period of tristeza virus can be identified prior to a routine detection method such as PCRbeing difficult to effectively detect the infected nursery stocks by combining a principal component analysis method. In a laboratory, samples are tested for 30 times, the detection result can reach 98%, during the field test, the samples are detected for 100 times, and the detection effect can reach 90%, so that the citrus tristeza virus can be effectively and rapidly detected.

Description

technical field [0001] The invention relates to a rapid detection method for citrus huanglongbing, belonging to the field of detection methods for plant diseases. Background technique [0002] Citrus huanglongbing is a devastating disease in citrus production, caused by bacteria that parasitize the phloem tissue of plants, including Phloembacterium asiatica, Phloembacterium africanica, and Phloembacterium americanum. After the plant is infected, the whole tree vigor is weakened, the growth is slow, the leaves are mottled and yellowed, the fruit is unevenly colored, and the symptoms of "red nose fruit" appear. -Withered within 8 years, seriously reducing the economic benefits of citrus. So far, there are no effective pesticides and resistant varieties against the disease, so early diagnosis of the disease is the basis and key to the prevention and control of citrus HLB. The real way to make a diagnosis is laboratory diagnosis, which requires section observation with the hel...

AI Technical Summary

Problems solved by technology

The real way to make a diagnosis is laboratory diagnosis, which requires section observation with the help of microscope technology, and there is a large possibility of missed detection; serology, nucleic acid hybridization and PCR and other methods can be detected when the bacterial concentration reaches a certain level, and during the incubation period of plant infection early stages of undetected

Images