Composite microbial agent for citrus tristeza virus, preparation method thereof and special biological agent for citrus tristeza virus prepared by using same

Abstract

The invention relates to the technical field of agricultural planting, in particular to a composite microbial agent for citrus tristeza virus, a preparation method thereof and a special biological agent for the citrus tristeza virus prepared by using the same. According to the invention, bacillus thuringiensis, beauveria bassiana and trichoderma harzianum are used to control the pathogen of citrustristeza virus pathogens on the ground and kill a spreading source wood louses and eggs thereof so as to effectively control the occurrence of the citrus tristeza virus, and four kinds of microorganism bacteria underground can effectively control the invasion of pathogenic microorganisms, effectively control the spread of pathogenic bacteria by nematodes, and simultaneously regulate the soil structure, decompose organic matters and improve the soil fertility. By promoting the growth of citrus roots with citrus specific AM fungi, citrus can grow new roots in clusters, the number of the new roots is enlarged, the tree vigor is enhanced, the immunity and disease resistance of trees are improved, a nitrogen fixation system is formed, and the pathogen control is combined with the improvement of the ecological environment for the growth of the citrus to achieve the effect of preventing and treating the citrus tristeza virus.

Description

technical field [0001] The invention relates to the technical field of agricultural planting, in particular to a compound microbial agent for citrus huanglongbing, a preparation method thereof and a special biological agent for citrus huanglongbing. Background technique [0002] At present, with changes in environmental conditions such as climate, soil, and human factors, citrus cultivation is facing more and more problems. Citrus diseases and insect pests are getting worse, especially citrus greening disease is getting more and more serious. In Guangdong, Guangxi, Jiangxi, Hunan, Fujian and other places, Huanglongbing disease is seriously harmful, and a large number of orchards are yellowed, losing production capacity; nematode damage is increasing year by year, seriously affecting the output of citrus . Various pests and diseases of citrus have caused huge economic losses to the citrus industry. [0003] Citrus huanglongbing is caused by a Gram-negative bacterium limited...

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Problems solved by technology

[0004] Most of the existing patents for the treatment of citrus huanglongbing use a single microorganism, such as patent 201110332473.8 (use and method for controlling citrus psyllids using Beauveria bassiana) discloses a method for controlling psyllids through Beauveria bassiana, but only superficially The transmission medium of citrus huanglongbing has been eliminated, but the threat of citrus soil-borne diseases has not been solved, and citrus huanglongbing has not been effectively treated fundamentally; such as patent 200910016337.0 (a compound culture of Trichoderma harzianum and its application in plant protection) , discloses a culture of Trichoderma harzianum compound bacteria and its application in plant protection, which achieves the effect of resisting the threat of various plant diseases, but the pertinence is not strong, and it is not effective against the psyllid, the vector of citrus greening disease. Control, can not effectively control the spread of the disease; such as patent 201610745978.X (a kind of microbial agent for preventing and treating Huanglongbing of kumquat and its preparation method), which discloses a microbial agent for preventing and treating Huanglongbing of kumquat and its preparation The method, the microbial agent is mixed in proportion after fermentation by Streptomyces Paleaceae, Streptomyces Cavuolus, Saccharomyces pyrolyticus, Bacillus Nissl, Trichoderma trichoderma, etc. The control of citrus huanglongbing has effectively solved the control of citrus huanglongbing, and it has a rhizosphere growth-promoting effect on citrus, but it has not been considered to improve the disease resistance and immunity of citrus from the perspective of less citrus root hairs and less cluster mycorrhizal formation. Although citrus The disease may be controlled, but citrus huanglongbing has a great impact on the overall tree vigor of citrus. Without a good root system, the tree vigor cannot be easily restored, and the effect on the recovery of citrus orchards is not significant.

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