One-step multi-PCR detection method for simultaneously detecting four important citrus pathogenies

A detection method, citrus technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problem of one-step multiple PCR system for grafting transmission diseases that have not yet been seen, achieve efficient and rapid detection, avoid repeated detection, The effect of ensuring safe production
CN102586481AInactive Publication Date: 2012-07-18CITRUS RES INST OF CHINESE ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
CITRUS RES INST OF CHINESE ACAD OF AGRI SCI
Publication Date
2012-07-18
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a one-step multi-PCR detection method for simultaneously detecting four important citrus pathogenies. According to the method, primers for the four important pathogenies including Citrus tristeza virus, Citrus exocortis viroid, Citrus tatter-leaf virus and Liberobacter asiaticum spreading through grafting are preferably selected, and primer concentration, PCR amplificationcondition and the like are optimized, therefore, the four important citrus pathogenies spreading through grafting can be simultaneously detected. The detection method provided by the invention is quick and accurate, has the advantages of good detection stability, high specificity and high consistency, is suitable for large-scale generalization, and can be used for guiding the introduction of varieties and generalization of detoxifying treatment of seedlings to ensure the safe production of citrus; in addition, by adopting the detection method, repeated detection in the conventional PCR is avoided.
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Description

technical field

[0001] The invention belongs to the field of agricultural molecular biology, and specifically relates to a one-step multiplex PCR detection method for simultaneously detecting four important graft-transmitted pathogens of citrus. Background technique

[0002] There are currently 5 kinds of graft-transmitted diseases identified in my country, including Huanglongbing, decaying disease, broken leaf disease, split skin disease and Wenzhou citrus shrunken disease. Due to the application of virus-free seedlings, the occurrence and distribution of Wenzhou satsuma shrunken disease in the field is rare. Citrus decline disease is an important disease caused by Citrus tristeza virus (CTV). The safety of grapefruit, sweet orange and other varieties sensitive to CTV stem pit strains. Citrus exocortis viroid is a worldwide important citrus disease caused by Citrus exocortis viroid (CEVd), and it is an important graft-transmissible viroid that limits the production of cit...

Claims

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