Citrus scion pretreatment technology for promoting removal of composite infection pathogens

A compound infection, pretreatment detoxification technology, applied in horticulture, botany equipment and methods, gardening tools/equipment, etc., can solve the problem of detoxification efficiency reduction and achieve high detoxification efficiency and good effect

Inactive Publication Date: 2014-07-02
HUAZHONG AGRI UNIV
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  • Abstract
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Problems solved by technology

[0008] The object of the present invention is to provide the technology that the pretreatment of citrus scion promotes the compound infection pathogen to remove, so...

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  • Citrus scion pretreatment technology for promoting removal of composite infection pathogens
  • Citrus scion pretreatment technology for promoting removal of composite infection pathogens
  • Citrus scion pretreatment technology for promoting removal of composite infection pathogens

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Embodiment Construction

[0023] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0024] Such as figure 1 Shown is the method step of the present invention, and the present invention mainly handles in two aspects:

[0025] (1) Detoxification of scion pretreatment: scion pretreatment is pretreatment with different concentrations of chemical small molecule bactericidal triclosan (TCL) and several antibiotics. Pathogen molecular detection of HLB and CTV, to explore the effect of different treatments on removing HLB and CTV pathogens, or reducing the concentration of pathogens. Screen out effective small molecule bactericidal or virucidal agents or effective antibiotics, and obtain the best treatment concentration and treatment method. After chemical treatment and detection, if the scion obtained is non-toxic, the seedlings can be directly propagated by conventional grafting. The time required to reproduce healthy seedlings...

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Abstract

The invention discloses a citrus scion pretreatment technology for promoting removal of composite infection pathogens. The citrus scion pretreatment technology includes acquiring citrus branches, pruning the citrus branches to remove leaves and leaf stalks and disinfecting the surfaces of the citrus branches by the aid of sodium hypochlorite (NaClO) aqueous solution; detecting molecules of scions of the citrus branches with the disinfected surfaces; performing detoxification treatment on scion materials by the aid of insecticides if the detected citrus branches are infected by HLB (huanglongbing) viruses and CTV (citrus tristeza viruses), acquiring virus-free shoots and completing the citrus scion pretreatment detoxification step. The citrus scion pretreatment technology has the advantages that the citrus branches are treated by the aid of small-molecule bactericide triclosan (TCL) and a plurality of antibiotics, so that virus-free stem tip materials without the citrus tristeza viruses (CTV) and huanglongbing (HLB) pathogens can be acquired; the detoxification efficiency is high, and a good effect can be realized; the virus-free stem tip materials can be provided for further stem tip micro-shoot grafting or tender-shoot grafting.

Description

technical field [0001] The invention belongs to the technical field of breeding of virus-free fine varieties, and relates to a technology for promoting removal of co-infected pathogens through pretreatment of citrus scion. Background technique [0002] Citrus detoxification technology is a key link to realize the breeding of virus-free varieties, and it is an essential supporting technology in the field of fruit tree cultivation, breeding and introduction and promotion. With climate change and the increase in the introduction and exchange of citrus varieties at home and abroad, the infection of citrus viruses and similar viruses is becoming more and more serious, and the combined infection situation is intensified. Therefore, it is important to study new, efficient and practical citrus detoxification technologies. Value. [0003] 1. Hazards of citrus viruses and similar viral diseases: The planting area and output of citrus are increasing year by year. During the cultivatio...

Claims

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Application Information

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IPC IPC(8): A01G13/00A01G1/00A01G7/00A01G31/00A01G1/06
Inventor 姜玲卢铭玉
Owner HUAZHONG AGRI UNIV
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