Hybridoma Cell Line Secreting Monoclonal Antibody Against Southern Bean Mosaic Virus and Application of Monoclonal Antibody

Abstract

The invention discloses a hybridoma cell strain capable of secreting an anti-Southern-bean-mosaic-virus monoclonal antibody and an application of the monoclonal antibody thereof. Purified Southern bean mosaic virus (SBMV) particles serve as antigen immunization BALB / c mice, and are subjected to cell fusion, cell screening and cell cloning, the hybridoma cell strain 19H9 capable of conducting stable passage and secreting the anti-SBMV monoclonal antibody is obtained, and the collection number is CGMCC No.12004. The ascites indirect ELISA titer of the monoclonal antibody secreted by the hybridoma cell strain is 10<-7>, and the type and the subgenera of the antibody are IgG1 light chains and kappa light chains. The monoclonal antibody and SBMV are subjected to a specific reaction. An ACP-ELISA method for detecting the SBMV in leguminous plants and a dot-ELISA method for detecting the SBMV in leguminous plants are built with the monoclonal antibody 19H9, and the diseased leaf detection sensitivity of the ACP-ELISA method and the dot-ELISA method is 1:81,920 w / v and 1:5,120 g / ml. The hybridoma cell strain 19H9, the obtained monoclonal antibody and the built serological detecting method provide substance supports and technical supports for diagnosing, detecting and scientific preventing and controlling of the virus disease.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a hybridoma cell strain secreting anti-Southern Bean Mosaic Virus monoclonal antibody and the application of the monoclonal antibody. Background technique [0002] Southern bean mosaic virus (SBMV) is a typical member of the genus Sobemovirus, mainly including two strains, SBMV-B and SBMV-C, and was first discovered and reported by Zaumeyer and Harter. The virus is mainly distributed in tropical and subtropical regions, and is also distributed in temperate regions. It can infect and harm soybeans, kidney beans, pumpkins and other plants. Bean mosaic virus disease in southern China generally occurs in the field, with many transmission routes and serious damage. Under natural conditions, it can cause mosaic and mottled diseases of kidney bean, cowpea, black mung bean and soybean, resulting in a decrease in the number of flowers, fruits and seeds, and a decrease in yield. Some countri...

AI Technical Summary

Problems solved by technology

At present, for SBMV port detection, biological detection, molecular biology detection and electron microscopy detection techniques are generally used. These methods are inefficient and not suitable for large-scale sample detection.

Serological methods are simple to operate, have good specificity, and can detect large-scale samples at the same time, but must rely on specific virus monoclonal antibodies

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