Hybridoma cell strain capable of secreting anti-apple stem grooving virus (ASGV) monoclonal antibody, antibody secreted by hybridoma cell strain, application of monoclonal antibody and kit prepared from monoclonal antibody

A technology of apple stem groove virus and hybridoma cell lines, applied in the field of broad-spectrum monoclonal antibodies, can solve the problems of high processing requirements, high throughput, poor specificity, etc., and achieve high titer, strong specificity, and stability Good results

Inactive Publication Date: 2014-12-24
INSPECTION & QUARANTINE TECH CENT SHANDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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Problems solved by technology

Among them, the ELISA method has a large throughput and low cost, but the antibody used is a polyclonal antibody, which has poor specificity and many false negatives, while the PCR-based detection method requires high pretreatment and high cost, especially not suitable for a large number of Sample screening

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  • Hybridoma cell strain capable of secreting anti-apple stem grooving virus (ASGV) monoclonal antibody, antibody secreted by hybridoma cell strain, application of monoclonal antibody and kit prepared from monoclonal antibody
  • Hybridoma cell strain capable of secreting anti-apple stem grooving virus (ASGV) monoclonal antibody, antibody secreted by hybridoma cell strain, application of monoclonal antibody and kit prepared from monoclonal antibody

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[0011] The hybridoma cell line ASGV secreting monoclonal antibodies against disease-resistant polystem groove virus was preserved in the China Center for Type Culture Collection (CCTCC) (Luojia, Wuchang, Wuhan City, Hubei Province) on April 26, 2014. Shan, Wuhan University, Collection Center), the collection number is CCTCC No: C201485, it can secrete monoclonal antibody against apple stem groove virus.

[0012] Anti-Apple Stem Groove Virus monoclonal antibody ascites indirect ELISA titer reached 10 -7 , the type and subclass of the antibody are IgG1 and kappa chain, and the monoclonal antibody can have a specific immune binding reaction with the coat protein subunit of apple stem groove virus.

[0013] The monoclonal antibody against apple stem groove virus has specific immune reaction only with apple stem groove virus, but with apple mosaic virus (Apple Mosaic Virus, ApMV), apple rust fruit viroid (Apple Scar Skin Viroid, ASSVd), apple green Apple Green Crinkle Virus (AgrCV...

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Abstract

The invention relates to a hybridoma cell strain capable of secreting an anti-apple stem grooving virus (ASGV) monoclonal antibody and application of the monoclonal antibody. The hybridoma cell strain is collected in China center for type culture collection (CCTCC), along with a collection name of hybridoma cell strain ASGV and a collection number of CCTCC NO:C201485. The hybridoma cell strain has the benefits as follows: firstly, the provided hybridoma cell strain ASGV is capable of secreting the anti-ASGV specific monoclonal antibody, and immunological methods of TAS-ELISA (three-antibody sandwich-enzyme-linked immuno sorbent assay), dot-ELISA and the like built by taking the monoclonal antibody as a core and a kit built by the methods are capable of highly specifically, accurately and sensitively detecting the ASGV; secondly, the prepared monoclonal antibody can be used for detecting the ASGV without expensive instruments and reagents; thirdly, the prepared monoclonal antibody can be used for effectively detecting the ASGV in ASGV hosts of imported nursery stocks and field apples, pears or the like.

Description

technical field [0001] The invention relates to a monoclonal antibody, a cell line secreting the antibody and application thereof. In particular, it relates to a broad-spectrum monoclonal antibody capable of neutralizing various genotypes of ASGV virus, a cell line secreting the monoclonal antibody and the application of the monoclonal antibody. Background technique [0002] Apple stem grooving virus (ASGV) is an important latent virus, the virus concentration in fruit trees is very low, and generally does not produce obvious symptoms, often causing serious economic losses to fruit tree production. The disease is prevalent all over the world. Apple stem groove virus usually causes chronic decline in apple trees, which can reduce yield by 45% to 73% and reduce fruit quality. When grafting with susceptible materials, it often leads to acute recession, causing devastating losses to production; latent infection is more common on pear trees, and when the rootstock is not resist...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/20C07K16/10G01N33/577G01N33/569C12R1/91
Inventor 魏晓棠尼秀媚白桦朱妍妍齐振华
Owner INSPECTION & QUARANTINE TECH CENT SHANDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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