Method for determining resistance of tomatoes to tomato yellow leaf curl viruses by injection inoculation

Abstract

The invention discloses a quick, simple and effective tomato yellow leaf curl virus resistance determination method and belongs to the field of plant protection. Traditionally, the determination of the tomato yellow leaf curl virus resistance is performed by a Bemisia tabaci (Gennadius) inoculation determination method, while the Bemisia tabaci (Gennadius) inoculation determination method has difficulty in providing uniform and consistent inoculation pressure due to the influences (such as the number of the inoculated Bemisia tabaci (Gennadius), virus rate and feeding habit) of the Bemisia tabaci (Gennadius) and at the same time, suffers interference from other Bemisia tabaci (Gennadius) transmitted viruses, so the accuracy of the determination result is low. The agrobacterium rhizogenes injection inoculation method well solves the problem. The resistance of a tomato material to the tomato yellow leaf curl viruses can be determined by using constructed infectious clones and directly inoculating the tomato yellow leaf curl viruses by injection in stalks. The method avoids culturing Bemisia tabaci (Gennadius), ensures simple operation, high efficiency, high repeatability and high controllability, provides a relatively consistent pressure, obtains accurate result and is a simple and effective inoculation determination method.

Description

technical field [0001] The invention relates to a method for identifying resistance to tomato yellow leaf curl virus, which belongs to the field of plant protection. Background technique [0002] Tomato yellow leaf curl is a devastating viral disease on tomato. Since the disease was first reported in 1964, it has been reported in Europe, Africa, Asia, the Caribbean and Oceania, especially in recent years. Recently, with the global warming and changes in farming systems and the increase in international trade, tomato yellow leaf curl has spread globally, causing serious losses to tomato production. In 1991-1992, the disease broke out on tomatoes in Florida, USA, causing losses of 140 million US dollars. [0003] In my country, the tomato yellow leaf curl disease caused by tomato yellow leaf curl virus (Tomato yellow leaf curl virus, TYLCV) was first reported in Shanghai and Zhejiang in 2006. After the tomato is infected by it, the leaf margin will appear early. Yellowing, ro...

AI Technical Summary

Problems solved by technology

Since tomato yellow leaf curl virus is a virus transmitted by whitefly, for a long time, the identification of the resistance of the virus has mostly used the whitefly inoculation identification method, but the method requires whitefly to complete the virus transmission during the operation process. Therefore there are some problems that are difficult to overcome: 1, the amount of inoculated insects of whitefly is difficult to control, because the life cycle of whitefly is very short, and generation overlapping phenomenon is very serious, causes its colony proliferation ability to be very strong, and the individual of whitefly It is very small, so no matter in the field or indoors, the number of whiteflies inoculated on a single plant is difficult to control; , which also leads to the difficulty in measuring the amount of inoculated insects of the poisonous whitefly; 3. The transmission efficiency of the whitefly is difficult to control, because the whitefly is a kind of poisonous insect, it can only be realized in the process of feeding. Virus transmission, and its feeding is affected by many factors, such as temperature, humidity, light, host, etc. These factors will affect the frequency and amount of feeding, affect the efficiency of virus transmission, and then affect the accuracy of identification results 4. Because whitefly spreads a lot of viruses, when whitefly is used to inoculate tomato yellow leaf curl virus, whitefly may carry other viruses at the same time, which will affect the results of inoculation identification

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