Method for identifying resistance of tomato to tomato yellow leaf curl virus by toothpick inoculation method
A technology for tomato yellow leaf curl and virus, which is applied in the directions of biochemical equipment and methods, botanical equipment and methods, applications, etc., and can solve the problems of small Bemisia tabaci, short life cycle of Bemisia tabaci, influence of poison transmission efficiency, etc. , to achieve the effect of accurate identification results, high efficiency and good repeatability
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[0016] The tomato materials 903 Dahong, 001, 002, and 747 were used as research objects, and their resistance to tomato yellow leaf curl virus was identified by toothpick inoculation method.
[0017] 1. Experimental treatment
[0018] a) Inoculate the Agrobacterium containing PTYj01 into the YEP liquid medium containing 50mg / ml Kanamycin and 50mg / ml Rifampicin (Rifamycin), and cultivate it to OD at 28°C and 150rpm 600 Reach 0.6-0.8.
[0019] b) Seedlings are grown under conventional conditions, moved to a disposable nutritional pot when there are 2 true leaves, and inoculated when there are 3-4 leaves.
[0020] c) Dip the tip of a toothpick to take the bacterial solution and stab 3-4 places in the phloem of the tomato stem at the 3-4 leaf stage (dip the bacterial solution once for each stab wound), and the treated tomatoes are stored in the greenhouse (at a temperature of 25 -28°C) for 1-2 days, and then moved to the field or greenhouse to observe the disease.
[0021] d) 1...
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