Preparation method, amplification primer and detection reagent of hpv full-length genome quality control product

Abstract

The invention relates to a preparation method of a HPV full-length genome quality control product, an amplification primer and a detection reagent. Wherein, the preparation method of the HPV full-length genome quality control product comprises the following steps: (1) obtaining a DNA template; (2) using amplification primers to perform PCR amplification; (3) using HD Cloning connection technology to connect the target DNA fragment to the carrier ; (4) introducing the plasmid into competent cells, and then extracting the plasmid; (5) diluting the plasmid with human genome. The preparation method of the human papillomavirus full-length genome quality control product of the present invention can successfully obtain the HPV gene detection quality control product that has no biological infection risk type, can simulate clinical samples, can be produced in large quantities, has a controllable concentration, and is stably stored. Has good applicability.

Description

technical field [0001] The invention relates to gene detection technology, in particular to a preparation method of HPV full-length genome quality control product, amplification primers and detection reagents. Background technique [0002] Human papillomavirus (Human Papillomavirus, HPV) is a group of non-enveloped small DNA viruses, belonging to Papillomaviridae. The morphological feature of the HPV virus is usually an icosahedron with a diameter of 52-55 nm. The length of its genomic DNA is about 8000bp, and the genome is functionally divided into three parts: including early transcription regions (coding early proteins such as E1, E2, E4, E5, E6, E7, etc., involved in virus replication, transcription, translation regulation and transformation, etc. function), late transcribed region (encodes major capsid protein L1 and minor capsid protein L2 for virus assembly; L1 accounts for about 80% of the capsid protein and is highly conserved, L2 has less content and more variatio...

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Problems solved by technology

[0009] The above-mentioned national standard materials are more expensive, the output is small, and the genotypes contained do not cover the common types of HPV39, 52, 68, etc., which cannot meet the standardization of reagents in my country, the performance evaluation of reagent methods in laboratories, indoor quality control and laboratory testing. The need for interim quality assessment

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