CYP450 gene type database and gene typing and enzymatic activity identification method

Abstract

The invention discloses a method for building a standard CYP450 (cytochrome p450) gene type database. The method comprises the steps as follows: comparing a specific sequence corresponding to mutation information of a CYP450 gene type with a standard human whole genome sequence to obtain a corresponding relation between the specific sequence and the standard human whole genome sequence; and converting the CYP450 gene type into a gene type taking the standard human whole genome sequence as a reference sequence according to the corresponding relation. The invention further discloses a database built by the method and a CYP450 gene typing and enzymatic activity identification method based on the database. The database is convenient for research of CYP450; the gene typing method provides unified standards for CYP450 gene typing and provides more accurate judgment criterions for diseases, drugs or the like; unknown mutation sites in CYP450 genes can be effectively detected; hundreds of samples can be detected at the same time; and the coverage range is comprehensive and wide.

Description

technical field

[0001] The invention relates to the field of gene detection, in particular to a CYP450 standard genotype database, a CYP450 genotyping method, and an enzyme activity identification method. Background technique

[0002] Cytochrome P450 is called CYP450 for short. At present, 57 kinds of CYP450 oxidases have been identified from the human body, among which the CYP1, CYP2 and CYP3 families involved in drug metabolism are mainly involved in the metabolism of more than 90% of the current drugs. The important role of CYP450 in drug metabolism makes CYP450 gene polymorphism one of the most important factors affecting individual differences in drugs, and its polymorphisms include point mutations, insertions or deletions, deletions or duplications of the entire gene, and ultimately lead to enzyme activity Enhanced, weakened or completely absent. CYP450 polymorphisms can lead to individual serious side effects or ineffectiveness at standard drug doses. For example, w...

Examples

Embodiment

[0092] The experimental procedure of this example is described in part as 50 samples including Yanhuang were built and hybridized to one chip. The number of samples in this example is used to explain the present invention, not to limit the number of samples that can be hybridized on each chip.

[0093] 1. Experimental materials

[0094] The reagents in this example are shown in Table 3, and other reagents, consumables, and equipment not indicated in Table 3 are all general-purpose products that can be purchased in the market.

[0095] Table 3 Reagents used in this embodiment

[0096]

[0097]

[0098] 2. Sequence capture library preparation

[0099] (1) Genomic DNA Fragmentation

[0100] 3 μg of Yanhuang genomic DNA without protein, RNA contamination and no degradation was used as the material, and the Covaris-S2 ultrasonic breaker (Covaris, US) was used for breaking. The interrupt parameters are set as follows:

[0101]

[0102]

[0103] After the truncated fr...

technical field

[0001] The invention relates to the field of gene detection, in particular to a CYP450 standard genotype database, a CYP450 genotyping method, and an enzyme activity identification method. Background technique

[0002] Cytochrome P450 is called CYP450 for short. At present, 57 kinds of CYP450 oxidases have been identified from the human body, among which the CYP1, CYP2 and CYP3 families involved in drug metabolism are mainly involved in the metabolism of more than 90% of the current drugs. The important role of CYP450 in drug metabolism makes CYP450 gene polymorphism one of the most important factors affecting individual differences in drugs, and its polymorphisms include point mutations, insertions or deletions, deletions or duplications of the entire gene, and ultimately lead to enzyme activity Enhanced, weakened or completely absent. CYP450 polymorphisms can lead to individual serious side effects or ineffectiveness at standard drug doses. For example, w...

Examples

Embodiment

[0092] The experimental procedure of this example is described in part as 50 samples including Yanhuang were built and hybridized to one chip. The number of samples in this example is used to explain the present invention, not to limit the number of samples that can be hybridized on each chip.

[0093] 1. Experimental materials

[0094] The reagents in this example are shown in Table 3, and other reagents, consumables, and equipment not indicated in Table 3 are all general-purpose products that can be purchased in the market.

[0095] Table 3 Reagents used in this embodiment

[0096]

[0097]

[0098] 2. Sequence capture library preparation

[0099] (1) Genomic DNA Fragmentation

[0100] 3 μg of Yanhuang genomic DNA without protein, RNA contamination and no degradation was used as the material, and the Covaris-S2 ultrasonic breaker (Covaris, US) was used for breaking. The interrupt parameters are set as follows:

[0101]

[0102]

[0103] After the truncated fr...

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