Method and application of constructing standard type database of drug-response-related genes

Abstract

The invention discloses a standard genotype database of drug response-related genes and its construction method. Firstly, the specific sequence corresponding to the mutation information is compared with the standard sequence of the whole human genome to obtain the corresponding relationship between the specific sequence and the standard sequence of the whole human genome ; Then, according to the corresponding relationship, the genotype is converted into a standard genotype relative to the standard sequence of the whole human genome. On this basis, the invention discloses a drug response-related genotype and a detection method for drug action. The database of the invention provides uniform standards for drug response-related genotypes and provides more accurate basis for clinical medication. Genotyping and drug action detection methods cover 48 human drug response-related genes, and can simultaneously detect multiple samples of all known mutation sites and corresponding drug information. The genotyping method can also detect unknown polymorphic sites, laying the foundation for research to discover new polymorphic sites that affect drug response.

Description

technical field [0001] The invention relates to the field of gene detection, in particular to a genotype database of drug response-related genes, a genotyping method for drug response-related genes, and a detection method for drug response effects. Background technique [0002] Individual variability in drug response is a common clinical problem. Many clinical drugs are only effective for some patients. It is estimated that the effective rate of asthma, cardiovascular and psychiatric drugs is about 60%, and as many as 40% of patients have unsatisfactory or even ineffective effects. At the same time, some patients are prone to adverse reactions to conventional treatment drugs. American epidemiological studies have shown that 6.7% of patients had severe side effects, of which 0.32% were fatal, which is the fourth to sixth leading cause of death among hospitalized patients. There are many factors that cause individual differences in drug response, including gender, age, body ...

AI Technical Summary

Problems solved by technology

At present, for drug-related gene polymorphism detection, such as PCR / RFLP, probe hybridization, etc., most of them have the shortcomings of few detection sites and low throughput. Among them, the latest technology for polymorphism detection is chip hybridization based on multiplex PCR. technology, but there are also defects that the number of detection sites is limited and the detection sites must be known

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