The invention provides a salmonella CRISPR (clustered regularlay interspaced short palindromic repeats) sequencing typing method; according to the method, by DNA sequencing of a latest interval sequence between Salmonella CRISPR1 site and CRISPR2 site, CRISPR type of a strain to be detected can be determined, and the Serotype can be predicted. The method is simple, rapid, low-cost and high-resolution, is high in consistency in salmonella serotyping results, and low in requirements on laboratory equipment and software, a new CRISPR type comprises bacterial evolution and regional and other various information, and by combination of a fluorescence probe and other methods, real time and rapid detection can be realized, and the method has popularization and application value.