The invention relates to the technical field of
molecular identification of pig varieties, and discloses an identification method of southern Anhui black pigs, which comprises the following steps: step 1, extracting
genome DNA of a sample to be detected; step 2, carrying out
genetic typing on the 16 SNP sites according to the claim 1 to obtain
genotype information of each SNP site; step 3, carrying out numerical coding on the
genotype information obtained in the step 2 to obtain a 16-dimensional
feature vector X of the sample to be detected; the rule of numerical coding is as follows: the homozygous coding of the reference
allele is 0, the heterozygous coding is 1, and the homozygous coding of the substitution
allele is 2; and step 4, performing
standardization processing on the
feature vector X to obtain a standardized
feature vector X '. According to the method, 16 specific SNPs are used as a core marker set, unified 0, 1 and 2 codes, standardized parameters (including a
mean vector mu and a standard
deviation vector sigma) and a threshold judgment rule are matched, a detection end process is clear,
repeatability is high, and consistent implementation among different laboratories is facilitated.