The invention discloses an anti-eavesdrop optimal secure transmission method based on collaboration interference, and the method comprises the following steps: S1, a transmitting source end Alice transmits a confidential message to a legal receiving end Bob with the help of a collaboration jammer Oscar, meanwhile, the transmitting source end Alice and a signal jammer Oscar generate interference signals, thus, interference on a single-antenna eavesdropping end Eve is realized, and a collaboration interference communication model is established; S2, according to the collaboration interference communication model, under a condition of satisfying confidentiality interruption constraint, optimal power distribution is realized, and a secure transmission scheme is determined. The invention provides the anti-eavesdrop optimal secure transmission method based on collaboration interference, in a collaboration interference communication process where channel estimation error exists, confidentiality capacity is maximized based on confidentiality interruption probability constraint, and optimization of secure transmission performance is realized.