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54 results about "Listeria ivanovii" patented technology
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Listeria ivanovii is a species of bacteria in the genus Listeria. The listeria are rod-shaped bacteria and become positively stained when subjected to Gram staining. Of the six bacteria species within the genus, L. ivanovii is one of the two pathogenic species (the other being L. monocytogenes). It behaves like L. monocytogenes, but is found almost exclusively in ruminants (mainly sheep). The species is named in honor of Bulgarian microbiologist Ivan Ivanov.
Annular mediated isothermal amplification-nano cadmium sulphide mark electrochemistry checking method for Listeria monocytogenes
InactiveCN101260424AMicrobiological testing/measurementMaterial analysis by electric/magnetic meansWater bathsLoop-mediated isothermal amplification
The invention relates to a method for detection Listeria monocytogenes with loop-mediated isothermal amplification-CdS nanoparticles labeling electrochemistry. The method is characterized in that the method comprises the following steps of: obtaining double-stranded target sequence of amplification reagent-Listeria monocytogenes to be detected by means of loop-mediated isothermal amplification reaction; pyrolyzing a double-stranded target sequence in water bath into a single-stranded target sequence, then self assembling and fixing at the surface of gold electrode, so that the target sequence modified electrode of the Listeria monocytogenes to be detected is obtained; afterwards, labeling the probe sequence coming from the Listeria monocytogenes by CdS nanoparticles to obtain a labeled probe sequence; carrying out molecular hybridization on the labeled probe sequence and the target sequence of the target sequence modified electrode, and carrying out electrochemical detection by simultaneity plating Hg anodic stripping method; detecting out cadmium ions corresponding to the species-specific genes (actA) of the detected Listeria monocytogenes. The method has the advantages of speediness, strong specificity, high sensitivity and convenient use.
Owner:QINGDAO UNIV OF SCI & TECH
Culture medium for composite enrichment of salmonella, Listeria monocytogenes and Staphylococcus aureus, and preparation thereof
InactiveCN101412978AShorten enrichment timeBacteriaMicrobiological testing/measurementLithium chlorideStaphylococcus cohnii
The invention discloses a complex enrichment medium used for salmonella, listeria monocytogenes and staphylococcus aureus and a method for preparing the same. The complex enrichment medium comprises the following components in weight portion: 15 to 19 portions of tryptone, 2 to 4 portions of peptone, 2 to 3 portions of sodium dihydrogen phosphate, 2 to 3 portions of glucose, 0.5 to 1.5 portions of aesculin, 1, 000 portions of distilled water, 1 to 2.5 portions of sodium pyruvate, 3 to 10.0 portions of mannitol, 1.0 to 25.0 portions of sodium chloride, 1 to 2.5 portions of lithium chloride, 0.0001 to 0.0002 portion of potassium tellurite, and 0.005-0.015 portion of nalidixic acid, wherein the pH value is between 7.1 and 7.5. The complex enrichment medium can inhibit the growth of other pathogenic microorganisms while performing enrichment on three target pathogens, thus the complex enrichment medium can be directly applied to the isolation culture and the biological assay test of target bacteria, and can also be directly applied to a detection technology of a plurality of pathogens based on one detection platform for multiple PCR and the like to make a diagnostic report.
Owner:SOUTH CHINA UNIV OF TECH
Plating media for the presumptive identification of Listeria sp, Listeria monocytogenes and Listeria ivanovii
InactiveUS20070259393A1Increase intensity colorMaterial can be affectedBacteriaUnicellular algaeListeria seeligeriAlglucerase
Isolation plating medium of a first color for the identification of Listeria sp. and Listeria monocytogenes and Listeria ivanovii containing a first chromogenic substrate that responds to phosphatidylinositol-specific phospholipase C enzymes to release precipitate of a second color into the medium and a second chromogenic substrate that responds to beta-glucosidase enzymes to release precipitate of a third color into the medium, and said first, second and third colors contrasting with each other.
Owner:R&F PROD
Preparation method of (Lactobacillus planetarium subsp. plantarum)Zhang-LL and its listeria monocytogene-resistant bacteriocin
ActiveCN104531562ASource securityStable sourceBacteriaMicroorganism based processesFood borneListeria murrayi
The invention relates to a preparation method of (Lactobacillus planetarium subsp.plantarum)Zhang-LL and its listeria monocytogene-resistant bacteriocin. The preparation method is suitable for preservation and fresh-keeping of meat and meat products, milk and milk products, fruits and vegetable, and instant foods. The (Lactobacillus planetarium subsp.plantarum)Zhang-LL (CGMCC No.6936) is selected from bacon on the Fujian farmer's market. A bacteriocin production broth is obtained by fermentation of a Zhang-LL strain, and the Zhang-LL strain bacteriocin is extracted and purified by a pH-dependent adsorption-desorption method, a cation exchange chromatography and a reversed phase high-performance liquid chromatography so that titer is improved by 32 times and purity is improved by 36.65 times. The bacteriocin can inhibit a plurality of food-borne pathogenic bacteria such as listeria monocytogenes, has high bacteriostatic activity, good heat, acid and base stability, can be degraded by human protease and is a natural and safe biological preservative. The preparation method has the advantages of simple processes, stability, high efficiency, source convenience, low cost and industrial production feasibility.
Owner:BEIJING BEINONG HONGZE BIOTECH CO LTD
Virulent phages to control listeria monocytogenes in foodstuffs and in food processing plants
ActiveUS20050175594A1Shorten the counting processGrowth inhibitionAntibacterial agentsBiocideBiotechnologyBacteroides
The present invention relates to virulent (lytic) Listeria monocytogenes phage from the Myoviridae family, preferably P100, alone or in combination with other virulent phages. P100 and the endolysin from P100 can be administered to food products, to the components that will be added to food products, and / or to the infrastructure of the food processing plants within which such food products are processed, or the containers or wraps in which such foods are stored and / or shipped, in order to reduce Listeria monocytogenes contamination. P100 can also be used in the present invention to identify Listeria monocytogenes bacteria present on (or within) foodstuffs, as well as those Listeria monocytogenes bacteria present in the equipment or the general environment of the food processing plants in which the foodstuffs are being processed and in animals infected with Listeria monocytogenes. The phage and the endolysin of the present invention can also be used to treat animals infected with Listeria monocytogenes. P100 will kill the bacteria that are within its host range with great efficiency and will propagate to high titer thereon. P100 can be combined with other lytic phage, and / or with other antimicrobial agents to reduce or eliminate Listeria.
Owner:EBI FOOD SAFETY
A kind of Lactobacillus pentosus, the fermentation product of the Lactobacillus and the application of the fermentation product
InactiveCN102286392AExtended shelf lifeLower pHBacteriaFood preservationMetaboliteStaphylococcus aureus
The invention relates to a lactobacillus pentosus strain and a fermentation product of the same. The preservation number of the lactobacillus pentosus LPEM818 in the invention is CGMCC No. 4583 in the China General Microbiological Culture Collection Center. The fermentation product of the lactobacillus pentosus in the invention can inhibit common pathogenic escherichia coli, pseudomonas aeruginosa and staphylococcus aureus in food, and particularly has a strong inhibiting effect on listeria monocytogenes which can cause serious food poisoning. In addition, the metabolite of the strain also can inhibit the growth of aspergillus flavus and cotton and tomato blight pathogens. Consequently, the lactobacillus pentosus strain and the fermentation product thereof play an active role in the fields of food preservation and plant protection.
Owner:ANHUI AGRICULTURAL UNIVERSITY
Detection of microbial metabolites
InactiveUS6051391ADisadvantageous effect stabilityGood colorMicroorganismsMicrobiological testing/measurementBacteroidesPhosphate
Owner:BIOSYNTH
Composite gene chip for food-borne pathogenic bacteria detection
InactiveCN101397586AExplicitly excludeClear and distinctMicrobiological testing/measurementAgainst vector-borne diseasesEscherichia coliFood borne
The invention relates to a composite gene chip for detecting food pathogenic microbes, which comprises eight parallel sub-arrays constructed by detection probe groups for detecting each microbe in the eight food pathogenic microbes, blank lines are respectively arranged among the eight sub-arrays to be as intervals, thus constructing a big array; the eight food pathogenic microbes are listeria monocytogenes, comma bacillus, vibrio parahaemolyticus, salmonella, shigella, staphylococcus aureus, campylobacter jejuni and escherichia coli. The gene chip can clearly and accurately detect the species of eight target food pathogenic microbes, and also can definitely exclude other off-target microbe samples out of the eight target food pathogenic microbes. Each food pathogenic microbe detection sub-array on the chip can clearly and definitely distinguish off-target microbe samples from target microbe samples.
Owner:广东省疾病预防控制中心
Salmonella, Listeria monocytogenes, bacillus coli multiple PCR rapid detection kit and uses thereof
InactiveCN101173315AEasy to operateStrong specificityMicrobiological testing/measurementAgainst vector-borne diseasesEscherichia coliBacteroides
The invention discloses a method simply and rapidly detecting Salmonella, Listeria monocytogenes producing monocyte, and escherichia coli simultaneously and a detection reagent kit invented based on the method. Firstly the DNA templates of the three microorganisms are extracted through hot dehiscing (Salmonella, escherichia coli) and enzymolysis method (Listeria monocytogenes), then the specific primers according to salmonella, Listeria monocytogenes producing monocyte, and escherichia coli are designed respectively, the practical amplification fragments are salmonella 320bp, Listeria monocytogenes producing monocyte 440bp, and escherichia coli 706bp. The invention has the advantages of celerity and nicety compared with the bacteria separation, identification of classical method and serological typing test method, strong specificity and high sensitivity compared with the generic multiplex PCR method, and the ability to detect and identify the three pathogens simultaneously.
Owner:CHINA AGRI UNIV
Selective growth medium for Listeria spp
ActiveUS7960164B2Quick and accurate identificationBacteriaMicrobiological testing/measurementBacteroidesLithium chloride
The invention is a selective growth medium for investigating, isolating, counting and directly identifying pathogenic bacteria of the genus Listeria.The medium promotes the Listeria spp. while simultaneously inhibiting the growth of non-Listeria organisms. The medium does not produce a bacterial biomass contaminated with interfering fluorophores. The medium contains nitrofurantoin, esculin and lithium chloride and lacks acriflavin.
Owner:PARADIGM DIAGNOSTICS
Listeria monocytogenes bacteriophages and uses thereof
ActiveUS8685697B1Prevent and eradicate and reduce level of colonizationMinimize and eliminate bacterial contaminationMicroorganismsFood preservationBiotechnologyListeria murrayi
The present invention is directed to isolated bacteriophages having specificity and lytic activity against strains of Listeria species, methods of using the bacteriophages, progeny and derivatives derived therefrom, to control the growth of Listeria species in various settings (e.g., food safety, sanitation, probiotics).
Owner:INTRALYTIX
Detection kit of 16 food born pathogenic bacteria
InactiveCN108220464ANo pollution in the processAvoid pollutionMicrobiological testing/measurementMicroorganism based processesEscherichia coliFood borne
The invention discloses a detection kit of 16 food born pathogenic bacteria. The detection kit comprises a PCR amplification reagent and reference reagents; the PCR amplification reagent is composed of PCR buffer solution A, enzyme system B, and a primer probe mixed solution of the 16 food-borne pathogenic bacteria; the reference reagents are mainly composed of a negative control and a positive control; the PCR buffer solution A contains 5*Buffer, 25mM dN(U)TPs; the enzyme system B is mainly composed of HotStart Hi Taq DNA polymerase and UNG anti-pollution enzyme; the primer probe mixed solution of the 16 food-borne pathogenic bacteria contains a mixed solution of detection primer probes of salmonella, Shigella, enteropathogenic Escherichia coli, enterotoxigenic E. coli, enteroinvasive escherichia coli, adherent escherichia coli, Escherichia coli O157, Staphylococcus aureus, listeria monocytogenes, Bacillus Cereus, campylobacter jejuni, vibrio parahaemolyticus, Vibrio cholerae group O1, Vibrio cholerae group O139, Yersinia enterocolitica, and enterobacter sakazakii. The detection kit is high in detection sensitivity, is simple, and is convenient.
Owner:HANGZHOU ZHUNXIN BIOTECHNOLOGY CO LTD
Kit for detecting Listeria monocytogenes and staphylococcus aureus, and application thereof
InactiveCN102676647AStrong specificityIncreased sensitivityMicrobiological testing/measurementMicroorganism based processesDiseaseFood regulation
Owner:NORTHEAST AGRICULTURAL UNIVERSITY
Polynucleotide, method and kit for detecting listeria monocytogenes
ActiveCN105177127AImprove uniformityImprove stabilityMicrobiological testing/measurementDNA preparationFluorescenceDNA construct
The invention discloses polynucleotide, a method and a kit for detecting listeria monocytogenes. Specifically, the invention discloses polynucleotide, which can be used as a standard molecule in realtime fluorescent PCR detection of listeria monocytogenes, and DNA constructs (LY01 and LY02). The provided plasmid standard molecule can solve the problem that realtime fluorescence PCR detection of listeria monocytogenes is lack of standard substances, and moreover, can guarantee the comparability of the detection results of realtime fluorescence PCR detection. A reliable quality control method is provided for the realtime fluorescence PCR detection of listeria monocytogenes.
Owner:SHANGHAI INST OF MEASUREMENT & TESTING TECH
Prokaryotic expression and purification method for listeria monocytogenes hemolysin O
InactiveCN101691582AQuick checkAccurate detectionMicroorganism based processesBiological testingEscherichia coliPurification methods
The invention discloses a prokaryotic expression and purification method for listeria monocytogenes hemolysin O, which comprises the following steps: 1) performing PCR augmentation, PCR electrophoresis and PCR recovery according to a gene sequence of listeria monocytogenes registered on an NCBI and a polyclone locus design primer of a vector PMD 18-T to be coupled, and performing a DNA coupling reaction on a PCR regenerant; 2) preparing and inverting colibacillus competent cells; 3) preparing a little plasmid DNA in an alkaline process; 4) recombining and constructing expression plasmids and inductively expressing recombinants; and 5) purifying the affinity chromatography and purification of expression protein. The listeria monocytogenes hemolysin O protein is prepared to acquire colloidal gold Immunochromatographic test strips prepared by antibodies, so that the listeria monocytogenes can be detected quickly and accurately in the process of food detection.
Owner:SHANGHAI ACAD OF AGRI SCI
Enrichment of Listeria spp
ActiveUS8501457B2Growth inhibitionIncrease probabilityBacteriaUnicellular algaeListeria floridensisGenus Listeria
A medium for enriching Listeria spp. without polymerase chain reaction (PCR) inhibition and a method of using the medium.
Owner:HANWHA AEROSPACE CO LTD
Duplex PCR detection kit and detection method for Listeria monocytogenes and Enterococcus faecium
ActiveCN104846067ADetection helpsAids in early diagnosisMicrobiological testing/measurementMicroorganism based processesLower limitDuplex pcr
The invention relates to a duplex PCR detection kit for Listeria monocytogenes and Enterococcus faecium and a detection method using the kit. The kit detects a primer pair of Listeria monocytogenes hlyA and a primer pair of the dd1 gene of an Enterococcus faecium strain; if specific amplification bands are obtained at 174 bp and 557 bp, Listeria monocytogenes and Enterococcus faecium are positive; and the kit can simultaneously detect Listeria monocytogenes and Enterococcus faecium. The lower limit of the detection sensitivity of the is lower than a minimum infective dose of pathogenic bacteria, which is of great significance to later clinical application and to monitoring of Listeria monocytogenes and Enterococcus faecium.
Owner:INNER MONGOLIA AUTONOMOUS REGION ACAD OF AGRI & ANIMAL HUSBANDRY SCI
Nonconformable listeria monocytogenes vaccine and antitumor immune response method
ActiveCN110408634AEasy to operateIncrease insertion efficiencyTumor rejection antigen precursorsBacteriaListeria seeligeriResponse method
The invention relates to a nonconformable listeria monocytogenes vaccine and an antitumor immune response method, in particular to a recombinant nucleic acid molecule, a recombinant plasmid or a recombinant expression carrier containing the recombinant nucleic acid molecule, recombinant protein and recombinant listeria monocytogenes. Besides, the invention further relates to a medical compositionor a vaccine containing the components, and an application thereof, and further provides a method for slowly and continuously killing cells and a method for inducing immune response in examinees. Theplasmid adopted in the invention is quite stable in the repeated passage processes of the listeria monocytogenes. The carrier obtained by the invention can be free from influence of restriction enzymecutting sites on a heterogenetic antigen, is convenient to operate, high in inserting efficiency and accurate to insert, the expression of antigen peptide of the nonconformable listeria monocytogenestumor vaccine is greatly increased, and the effect of the carrier on the antitumor immune response is more significant.
Owner:SUZHOU ROYALTECH MED CO LTD +1
Culture medium with simultaneoulsy enriched salmonella, colibacillus and Staphylococcus aureus
InactiveCN101045909ARapid growthImprove accuracyBacteriaMicroorganism based processesDiseaseEscherichia coli
The present invention relates to one kind of culture medium with simultaneously enriched salmonella, colibacillus and Staphylococcus aureus, and belongs to the field of food originated pathogenic bacterium detecting technology. The culture medium with simultaneously enriched salmonella, colibacillus and Staphylococcus aureus consists of peptone 10 g, beef extract 3 g, sodium chloride 50 g, Na2HPO4.12H2O 9 g, potassium dihydrogen phosphate 1.5 g and distilled water 1000 ml, and has pH 7.2.
Owner:陈福生 +1
Virulent phages to control Listeria monocytogenes in foodstuffs and in food processing plants
ActiveUS7438901B2Shorten the counting processIncreased substrate specificityAntibacterial agentsBiocideBiotechnologyBacteroides
The present invention relates to virulent (lytic) Listeria monocytogenes phage from the Myoviridae family, preferably P100, alone or in combination with other virulent phages. P100 and the endolysin from P100 can be administered to food products, to the components that will be added to food products, and / or to the infrastructure of the food processing plants within which such food products are processed, or the containers or wraps in which such foods are stored and / or shipped, in order to reduce Listeria monocytogenes contamination. P100 can also be used in the present invention to identify Listeria monocytogenes bacteria present on (or within) foodstuffs, as well as those Listeria monocytogenes bacteria present in the equipment or the general environment of the food processing plants in which the foodstuffs are being processed and in animals infected with Listeria monocytogenes. The phage and the endolysin of the present invention can also be used to treat animals infected with Listeria monocytogenes. P100 will kill the bacteria that are within its host range with great efficiency and will propagate to high titer thereon. P100 can be combined with other lytic phage, and / or with other antimicrobial agents to reduce or eliminate Listeria.
Owner:EBI FOOD SAFETY
Composite enrichment medium for Escherichia coli, Salmonella and Listeria monocytogenes
InactiveCN109971683AEasy to prepareIsokinetic proliferationBacteriaMicroorganism based processesEscherichia coliDipotassium phosphate
The invention discloses a composite enrichment medium for Escherichia coli, Salmonella and Listeria monocytogenes and a preparation method thereof. The composite enrichment medium for Escherichia coli, Salmonella and Listeria monocytogenes comprises 16.0-18.0 parts of tryptone, 2.0-4.0 parts of peptone, 5.0-7.0 parts of yeast extract, 10.0-20.0 parts of sodium chloride, 2.4-2.6 parts of dipotassium phosphate, 9.5-9.7 parts of disodium hydrogen phosphate, 1.34-1.36 parts of monopotassium phosphate, 0.005-0.020 part of acriflavine, 2.0-8.0 pars of magnesium chloride, 1.0-4.0 parts of glucose, 1.0-4.0 parts of sodium pyruvate and 1000 parts of distilled water, and has pH of 6.8-7.4. The composite enrichment medium herein allows three target pathogens to be enriched at a high, constant speed;after 16-24 hours of enrichment, quick detection of the three target pathogens can be implemented to provide a diagnostic report through high-flux detection devices, such as multiple PCR (polymerase chain reaction), multi-channel SPR (surface plasma resonance) biosensors, and multi-channel BLI (biolayer interferometry) biosensors.
Owner:JILIN UNIV
Multiple PCR detection kit for Listeria monocytogenes and Listeria illichii
ActiveCN110184367AStrong specificityHigh sensitivityMicrobiological testing/measurementMicroorganism based processesListeria murrayiMicrobiology
The invention provides a multiple PCR detection kit for Listeria monocytogenes and Listeria illichii. The kit comprises a gene lmo0601 detection primer and a gene smcl detection primer. The inventionestablishes a multiple PCR method for rapidly detecting Listeria monocytogenes and Listeria illichii by using the two pairs of primers, and the method has the advantages of good specificity, high sensitivity, high detection speed, easy operation and simple result judgment.
Owner:YANGZHOU UNIV
A kind of Lactobacillus plantarum plant subspecies and its anti-Listeria monocytogenes bacteriocin preparation method
ActiveCN104531562BSource securityStable sourceBacteriaMicroorganism based processesOrganismPh dependent
The invention relates to a Lactobacillus plantarum plant subspecies and a preparation method for producing anti-Listeria monocytogenes bacteriocin, which are suitable for the preservation of meat and meat products, milk and dairy products, fruits and vegetables, and ready-to-eat food. In the present invention, Lactobacillus plantarum subsp. plantarum Zhang-LL (CGMCC No. 6936) is obtained by screening the bacon in the Fujian farmers market. Bacteriocin-producing fermentation broth was obtained by fermentation of Zhang-LL strain, and the bacteriocin of Zhang-LL strain was extracted and purified by pH-dependent adsorption-desorption method, cation exchange chromatography, and reversed-phase high performance liquid chromatography. It is 32 times higher and the purity is 36.65 times higher. The bacteriocin can inhibit various food-borne pathogens such as Listeria monocytogenes, has high antibacterial activity, is stable to heat and acid and alkali, and is degraded by human protease, so it is a natural and safe biological preservative. The method has the advantages of simple operation, stability, high efficiency, convenient source and low cost, and is suitable for industrial production.
Owner:BEIJING BEINONG HONGZE BIOTECH CO LTD
Detection method for listeria monocytogenes
InactiveCN105842442AReduce fluorescence quenchingSensitive detectionBiological material analysisBiotin-streptavidin complexDecomposition
The invention provides a detection method for listeria monocytogenes (LM). The method comprises the steps of firstly combining the LM with a coated monoclonal antibody, then connecting with a biotinylated polyclonal antibody, next connecting with a streptavidin labeled catalase C100, catalyzing decomposition of hydrogen peroxide by the catalase, reducing fluorescence quenching of mercaptopropionic acid modified cadmium telluride quantum dots, and determining the concentration of the LM in a sample according to the level of the fluorescence intensity. The method is based on the double-antibody sandwich enzyme-linked immunosorbent assay, and a biotin-avidin system is used for amplification of the reaction. More importantly, due to use of the new antibody labeled enzyme (catalase C100) and the more sensitive fluorescent substrate (cadmium telluride quantum dots), and matching of the effective reaction conditions, the detection sensitivity is significantly improved, at the same time, the cost is reduced, and the detection efficiency is improved; therefore, the detection method has good prospects for promotion.
Owner:NANCHANG UNIV
Composite gene chip for food-borne pathogenic bacteria detection
InactiveCN101397586BExplicitly excludeClear and distinctMicrobiological testing/measurementAgainst vector-borne diseasesEscherichia coliFood borne
Owner:广东省疾病预防控制中心
Dual PCR method for simultaneously detecting Listeria monocytogene and Listeria ivanovii
InactiveCN107988330AStrong specificityEfficient detectionMicrobiological testing/measurementMicroorganism based processesDuplex pcrFluorescence
The invention discloses a dual PCR method for simultaneously detecting Listeria monocytogene and Listeria ivanovii, and belongs to the technical field of detection of foodborne pathogenic bacteria. Primers and probes are designed in allusion to a newly discovered Listeria monocytogene-specific gene LMOf2365_0970 and a newly discovered Listeria ivanovii-specific gene AX25_00730; by the detection method, the Listeria monocytogene and the Listeria ivanovii in food can be effectively detected; the detection limit for the two target bacteria is N*102 CFU / mL, and the fluorescence quantitative PCR detection time is shorter than 40 minutes; the detection method has the characteristics of high detection sensitivity, good specificity and strong anti-interference ability; by the detection method, quick qualitative and accurate quantitative detection of the Listeria monocytogene and the Listeria ivanovii in food can be achieved, and the purpose of rapidly screening contaminated food samples is achieved.
Owner:NANJING AGRICULTURAL UNIVERSITY
Listeria-based EphA2 vaccines
The present invention relates to methods and compositions designed to treat, control or prevent cancer, particularly metastatic cancer and cancer of T cell origin, and hyperproliferative diseases associated with EphA2 expressing cells. The methods of the invention comprise the use of a Listeria-based EphA2 vaccine. The invention also provides pharmaceutical compositions comprising one or more Listeria-based vaccines of the invention, alone or in combination with one or more other agents useful in the treatment of cancer, in certain embodiments of the invention. In some aspects, the method comprises eliciting CD4 against EphA2 and / or EphA2 expressing cells + and CD8 + T cell response.
Owner:MEDIMMUNE LTD +1
Methods and oligonucleotides for the detection of Salmonella SP., E coli 0157:H7, and Listeria monocytogenes
InactiveUS20060246463A1Sensitive methodMethod is fastMicrobiological testing/measurementFermentationEscherichia coliListeria murrayi
A method for detecting a Salmonella species, E. coli 0l7:H7, or Listeria monocytogenes is disclosed. The method involves amplifying a genomic nucleotide sequence of a corresponding species and detecting the amplification product. Various primers and probes that can be used in the method are also disclosed. In one embodiment, the amplification step of the method is accomplished by real-time PCR and the amplification product is detected by fluorescence resonance energy transfer using a pair of labeled polynucleotides.
Owner:VEVEA DIRK +1
Cervical cancer therapeutic vaccine based on recombinant attenuating sheep listeria ivanovii
ActiveCN108939064ABreak immune toleranceMake up for the disadvantage of lower immunogenicityBacteriaViral antigen ingredientsAbnormal tissue growthListeria floridensis
The invention relates to a cervical cancer therapeutic vaccine based on recombinant attenuating sheep listeria ivanovii. By taking the attenuating sheep listeria ivanovii as a vector, specific immuneresponse in a tumor microenvironment is improved effectively and immune tolerance of a body is broken by means of a unique characteristic that the listeria ivanovii can grow in a main phagocyte and isa natural T cell immune activation adjuvant, so that continuous virus infection of the body is eliminated and the good therapeutic effect of eliminating the focus and inhibiting tumor development isachieved. The vaccine has the advantages that the defect that nucleic acid and protein polypeptide vaccines are relatively low in immunogenicity is made up, and the attenuating sheep listeria ivanoviialso has high safety while retaining the characteristic that an original strain can grow in cells to achieve antigen presentation.
Owner:南京颂悦生物科技有限公司
Listeria monocytogenes chromogenic medium and test sheet
InactiveCN106498023ALow costReduce usageMicrobiological testing/measurementLithium chlorideMaterial resources
The invention discloses a Listeria monocytogenes chromogenic medium and test sheet. The medium comprises peptone, yeast extract powder, sodium chloride, sodium pyruvate, lithium chloride, magnesium glycerophosphate, a specific enzyme chromogenic substrate and a competitor inhibitor. The chromogenic medium has the advantages of small use amount of the specific enzyme chromogenic substrate, great reduction of the cost, simple preparation method, simple sterilization steps, simplification of requirements of sterilization conditions, and high facilitation of use of base detection departments. The test sheet is convenient to use, and detection of Listeria monocytogenes can be completed after sample addition and constant temperature culture at 37 DEG C for 24-36 h, so a large amount of manpower and material resources can be saved.
Owner:GUANGDONG DAYUAN OASIS FOOD SAFETY TECH CO LTD
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