54 results about "Listeria ivanovii" patented technology

A method of detecting a phosphatidylinositol-specific phospholipase C enzyme by means of a substrate which is cleaved by said enzyme and yields a dye when the chromophoric portion of the substrate is dimerized and oxidized; the invention teaches using in such method, as a novel substrate, a 3-indoxyl-myoinositol-1-phosphate compound of formula (I) wherein R is selected from the group consisting of hydrogen and C1-4 alkyl, while R1, R2, R3, and R4 are radicals selected from the group consisting of hydrogen and chromogenic substituents, or of a salt of said formula I compound. The invention provides for a safe, sensitive and commercially viable detection of potentially pathogenic bacterial activity of such microbes as Bacillus cereus, B. Thuringiensis, Staphylococcus aureus and various Listeria strains in potentially infected materials including physiological samples or consumable goods such as foods and beverages.

The invention is a selective growth medium for investigating, isolating, counting and directly identifying pathogenic bacteria of the genus Listeria.The medium promotes the Listeria spp. while simultaneously inhibiting the growth of non-Listeria organisms. The medium does not produce a bacterial biomass contaminated with interfering fluorophores. The medium contains nitrofurantoin, esculin and lithium chloride and lacks acriflavin.

The present invention is directed to isolated bacteriophages having specificity and lytic activity against strains of Listeria species, methods of using the bacteriophages, progeny and derivatives derived therefrom, to control the growth of Listeria species in various settings (e.g., food safety, sanitation, probiotics).

A medium for enriching Listeria spp. without polymerase chain reaction (PCR) inhibition and a method of using the medium.

The present invention relates to virulent (lytic) Listeria monocytogenes phage from the Myoviridae family, preferably P100, alone or in combination with other virulent phages. P100 and the endolysin from P100 can be administered to food products, to the components that will be added to food products, and / or to the infrastructure of the food processing plants within which such food products are processed, or the containers or wraps in which such foods are stored and / or shipped, in order to reduce Listeria monocytogenes contamination. P100 can also be used in the present invention to identify Listeria monocytogenes bacteria present on (or within) foodstuffs, as well as those Listeria monocytogenes bacteria present in the equipment or the general environment of the food processing plants in which the foodstuffs are being processed and in animals infected with Listeria monocytogenes. The phage and the endolysin of the present invention can also be used to treat animals infected with Listeria monocytogenes. P100 will kill the bacteria that are within its host range with great efficiency and will propagate to high titer thereon. P100 can be combined with other lytic phage, and / or with other antimicrobial agents to reduce or eliminate Listeria.

The invention provides a multiple PCR detection kit for Listeria monocytogenes and Listeria illichii. The kit comprises a gene lmo0601 detection primer and a gene smcl detection primer. The inventionestablishes a multiple PCR method for rapidly detecting Listeria monocytogenes and Listeria illichii by using the two pairs of primers, and the method has the advantages of good specificity, high sensitivity, high detection speed, easy operation and simple result judgment.

The invention relates to a Lactobacillus plantarum plant subspecies and a preparation method for producing anti-Listeria monocytogenes bacteriocin, which are suitable for the preservation of meat and meat products, milk and dairy products, fruits and vegetables, and ready-to-eat food. In the present invention, Lactobacillus plantarum subsp. plantarum Zhang-LL (CGMCC No. 6936) is obtained by screening the bacon in the Fujian farmers market. Bacteriocin-producing fermentation broth was obtained by fermentation of Zhang-LL strain, and the bacteriocin of Zhang-LL strain was extracted and purified by pH-dependent adsorption-desorption method, cation exchange chromatography, and reversed-phase high performance liquid chromatography. It is 32 times higher and the purity is 36.65 times higher. The bacteriocin can inhibit various food-borne pathogens such as Listeria monocytogenes, has high antibacterial activity, is stable to heat and acid and alkali, and is degraded by human protease, so it is a natural and safe biological preservative. The method has the advantages of simple operation, stability, high efficiency, convenient source and low cost, and is suitable for industrial production.

The invention relates to a composite gene chip for detecting food pathogenic microbes, which comprises eight parallel sub-arrays constructed by detection probe groups for detecting each microbe in the eight food pathogenic microbes, blank lines are respectively arranged among the eight sub-arrays to be as intervals, thus constructing a big array; the eight food pathogenic microbes are listeria monocytogenes, comma bacillus, vibrio parahaemolyticus, salmonella, shigella, staphylococcus aureus, campylobacter jejuni and escherichia coli. The gene chip can clearly and accurately detect the species of eight target food pathogenic microbes, and also can definitely exclude other off-target microbe samples out of the eight target food pathogenic microbes. Each food pathogenic microbe detection sub-array on the chip can clearly and definitely distinguish off-target microbe samples from target microbe samples.

The invention discloses a Listeria monocytogenes chromogenic medium and test sheet. The medium comprises peptone, yeast extract powder, sodium chloride, sodium pyruvate, lithium chloride, magnesium glycerophosphate, a specific enzyme chromogenic substrate and a competitor inhibitor. The chromogenic medium has the advantages of small use amount of the specific enzyme chromogenic substrate, great reduction of the cost, simple preparation method, simple sterilization steps, simplification of requirements of sterilization conditions, and high facilitation of use of base detection departments. The test sheet is convenient to use, and detection of Listeria monocytogenes can be completed after sample addition and constant temperature culture at 37 DEG C for 24-36 h, so a large amount of manpower and material resources can be saved.