The invention discloses a vector containing a double-target chimeric antigen receptor gene, a CAR-T cell and application of the CAR-T cell. The double-target chimeric antigen receptor is formed by sequentially splicing signal peptides, CD44 single-chain antibody ScFv, EpCAM single-chain antibody ScFv, strepII tag, CD8 molecular hinge region, CD28 molecular transmembrane region, intracellular structural domain of the CD28 molecular transmembrane region, intracellular costimulatory domain 4-1BB and CD3 molecular zeta chain from an N end to a C end. Meanwhile, a lentivirus expression recombinantplasmid pTK-881-CD44-EpCAM is constructed, lentivirus packaging is carried out, T cells are infected, and the double-target-point CAR-T cells capable of removing CD44 and EpCAM at the same time are obtained. Based on a CAR-T technology, gastric cancer stem cells are killed by only utilizing autoimmunity of a patient, so that a doctor is assisted in gastric cancer treatment.