Method for preparing tumor-specific DC vaccine by applying CD34+ cells of umbilical cord blood
A tumor-specific, cell-based technology, which is applied in the field of preparation of tumor-specific DC vaccines using umbilical cord blood CD34+ cells, can solve the problems of restricting clinical applications, mass production of DC vaccines, and industrial production of DC vaccine preparations.
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[0069] ①. Fresh tumor tissue resected by surgery: take 0.3-1.0 cm of tissue around the tumor (to avoid necrotic tissue in the center of the tumor) 3 (It can be stored in liquid nitrogen or -80°C refrigerator for later use), cut off the surrounding non-tumor tissue, washed 3-5 times with gentamicin-normal saline, cut into pieces, grinded with a 300-mesh steel mesh to prepare a single-cell suspension, and freeze-thawed repeatedly The tumor cell lysate was prepared by the method, and the protein was quantified after passing through the mesh for later use.
[0070] ②Tumor cells obtained from pleural and ascites: Take 1500-2000ml of pleural and ascites, centrifuge at 1200rpm, wash and centrifuge with normal saline for 3 times, pass through a 300-mesh steel mesh to obtain a single cell suspension, and prepare tumor cell lysate by repeated freezing and thawing , protein quantification after passing through the screen for later use.
[0071] ③. Cell lines derived from the same tissue...
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