Composite gene chip for food-borne pathogenic bacteria detection

Abstract

The invention relates to a composite gene chip for detecting food pathogenic microbes, which comprises eight parallel sub-arrays constructed by detection probe groups for detecting each microbe in the eight food pathogenic microbes, blank lines are respectively arranged among the eight sub-arrays to be as intervals, thus constructing a big array; the eight food pathogenic microbes are listeria monocytogenes, comma bacillus, vibrio parahaemolyticus, salmonella, shigella, staphylococcus aureus, campylobacter jejuni and escherichia coli. The gene chip can clearly and accurately detect the species of eight target food pathogenic microbes, and also can definitely exclude other off-target microbe samples out of the eight target food pathogenic microbes. Each food pathogenic microbe detection sub-array on the chip can clearly and definitely distinguish off-target microbe samples from target microbe samples.

Description

technical field [0001] The invention belongs to the field of food safety and relates to a composite gene chip for detecting food-borne pathogenic bacteria. Background technique [0002] Food poisoning refers to non-infectious (not infectious) acute and subacute diseases that occur after ingesting food containing biological or chemical toxic and harmful substances or taking toxic and harmful substances as food. According to the pathogenic substances, food poisoning can be divided into five types: bacterial, chemical, animal, plant, and fungal, among which bacterial and chemical food poisoning are the most common. Bacterial food poisoning refers to a disease with acute gastroenteritis as the main symptom of poisoning caused by eating food contaminated by pathogenic bacteria or their toxins. It is the most common type of food poisoning. Common pathogens causing bacterial food poisoning are: Vibrio parahaemolyticus, Salmonella, Staphylococcus, Escherichia coli, Vibrio cholerae,...

AI Technical Summary

Problems solved by technology

The food-borne pathogen detection chips reported so far either detect a single strain of bacteria, or detect a single gene, or the purpose of the detection chip is not clear, and none of them reflect the characteristics of high throughput and high information content of the gene chip; moreover, These studies have no obvious personality and characteristics, and basically select the same or similar genes as the target genes for detection

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