Salmonella, Listeria monocytogenes, bacillus coli multiple PCR rapid detection kit and uses thereof
A detection kit and Salmonella technology, which is applied in the direction of recombinant DNA technology, microbial measurement/inspection, DNA/RNA fragments, etc., can solve the problems of cumbersome inspection methods, inconvenient import and export, and low specificity, and achieve high sensitivity and operation Simple, well-specific effects
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[0031] specific implementation plan
[0032] 1. Collect samples to be tested and cultivate pathogenic bacteria
[0033] Collect the samples to be tested, pay attention to avoid cross-contamination before and after collection, inoculate them in broth culture medium at 37°C for 18 hours (to cultivate Salmonella), and culture them in BHI (purchased from Difo, USA, to cultivate Listeria) overnight. LB medium (cultivation of Escherichia coli) was cultured overnight at 37°C.
[0034] And the cultured bacterial liquid is carried out as follows:
[0035] Escherichia coli and Salmonella: take 0.5mL bacterial culture solution, put it in an Eppendorf tube, centrifuge at 2000rpm for 20min, wash twice with sterilized distilled water, and finally suspend with 1mL distilled water, boil in a water bath, centrifuge at 8000rpm for 15min, take the supernatant, and obtain Template solutions for E. coli and Salmonella.
[0036] Listeria: Add 250ul of overnight culture into an Eppendorf tube, ce...
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