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Culture medium for composite enrichment of salmonella, Listeria monocytogenes and Staphylococcus aureus, and preparation thereof

A technology for Listeria monocytogenes and Salmonella, which is applied in the direction of biochemical equipment and methods, measurement/testing of bacteria and microorganisms, etc., can solve the problems of unsuitable high background microbial raw materials, lack of inhibitors, etc., and shorten the enrichment time Effect

Inactive Publication Date: 2009-04-22
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Pre-enrichment broth such as nutrient broth is economical, but lacks inhibitors to select target bacteria, not suitable for raw materials with high background microorganisms and untreated samples

Method used

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  • Culture medium for composite enrichment of salmonella, Listeria monocytogenes and Staphylococcus aureus, and preparation thereof
  • Culture medium for composite enrichment of salmonella, Listeria monocytogenes and Staphylococcus aureus, and preparation thereof
  • Culture medium for composite enrichment of salmonella, Listeria monocytogenes and Staphylococcus aureus, and preparation thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Weigh tryptone 17g, peptone 3g, sodium dihydrogen phosphate 2.5g, glucose 2.5g, escin 1g, sodium pyruvate 2.5g, mannitol 5g, sodium chloride 20g, lithium chloride 2g, distilled water and add to 990ml, Heat to dissolve, cool to room temperature and correct the pH to 7.2-7.3; mix well, sterilize at 121°C for 15 minutes; weigh 10 mg of nalidixic acid and add it to 5 ml of sodium hydroxide solution with a concentration of 0.05 mol / L to obtain naphthyridine Keto acid solution. Weigh 0.15 mg of potassium tellurite into 5 ml of sterilized distilled water to prepare a potassium tellurite solution. Under aseptic conditions, add 5 ml of nalidixic acid solution and 5 ml of potassium tellurite solution; subpackage and store at 4°C for later use, to prepare a compound enrichment for Salmonella, Listeria monocytogenes and Staphylococcus aureus. culture medium.

[0021] Dilute the prepared medium for Salmonella, Listeria monocytogenes and Staphylococcus aureus compound enrichment to...

Embodiment 2

[0026] Weigh tryptone 17g, peptone 3g, sodium dihydrogen phosphate 2.5g, glucose 2.5g, escin 1g, sodium pyruvate 2.0g, mannitol 10g, sodium chloride 25g, lithium chloride 2.5g, distilled water and add to 990ml , heated to dissolve, cooled to room temperature, and corrected the pH value to 7.2-7.3; mixed well, sterilized at 121 °C for 15 min; weighed 15 mg of nalidixic acid and added it to 5 ml of sodium hydroxide solution with a concentration of 0.05 mol / L to obtain nalidixic acid Pyridonic acid solution. Weigh 0.2 mg of potassium tellurite into 5 ml of sterilized distilled water to prepare a potassium tellurite solution. Under aseptic conditions, add 5 ml of nalidixic acid solution and 5 ml of potassium tellurite solution; subpackage and store at 4°C for later use, to prepare a compound enrichment for Salmonella, Listeria monocytogenes and Staphylococcus aureus. culture medium.

[0027] Dilute the prepared medium for Salmonella, Listeria monocytogenes and Staphylococcus aur...

Embodiment 3

[0032] Weigh tryptone 17g, peptone 3g, sodium dihydrogen phosphate 2.5g, glucose 2.5g, escin 1g, sodium pyruvate 1g, mannitol 3g, sodium chloride 10g, lithium chloride 2.5g, distilled water and add to 990ml, Heat to dissolve, cool to room temperature and correct the pH to 7.2-7.3; mix well, sterilize at 121 °C for 15 minutes; weigh 5 mg of nalidixic acid and add it to 5 ml of sodium hydroxide solution with a concentration of 0.05 mol / L to obtain naphthyridine Keto acid solution. Weigh 0.05 mg of potassium tellurite and add it to 5 ml of sterilized distilled water to prepare a potassium tellurite solution. Under aseptic conditions, add 5 ml of nalidixic acid solution and 5 ml of potassium tellurite solution; subpackage and store at 4°C for later use, to prepare a compound enrichment for Salmonella, Listeria monocytogenes and Staphylococcus aureus. culture medium.

[0033] Dilute the prepared medium for Salmonella, Listeria monocytogenes and Staphylococcus aureus compound enri...

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Abstract

The invention discloses a complex enrichment medium used for salmonella, listeria monocytogenes and staphylococcus aureus and a method for preparing the same. The complex enrichment medium comprises the following components in weight portion: 15 to 19 portions of tryptone, 2 to 4 portions of peptone, 2 to 3 portions of sodium dihydrogen phosphate, 2 to 3 portions of glucose, 0.5 to 1.5 portions of aesculin, 1, 000 portions of distilled water, 1 to 2.5 portions of sodium pyruvate, 3 to 10.0 portions of mannitol, 1.0 to 25.0 portions of sodium chloride, 1 to 2.5 portions of lithium chloride, 0.0001 to 0.0002 portion of potassium tellurite, and 0.005-0.015 portion of nalidixic acid, wherein the pH value is between 7.1 and 7.5. The complex enrichment medium can inhibit the growth of other pathogenic microorganisms while performing enrichment on three target pathogens, thus the complex enrichment medium can be directly applied to the isolation culture and the biological assay test of target bacteria, and can also be directly applied to a detection technology of a plurality of pathogens based on one detection platform for multiple PCR and the like to make a diagnostic report.

Description

technical field [0001] The invention belongs to a pre-enrichment medium for pathogenic bacteria, in particular to a medium for compound enrichment of Salmonella, Listeria monocytogenes and Staphylococcus aureus at the same time and a preparation method thereof. Background technique [0002] my country is the largest producer of animal husbandry in the world, and its meat output ranks first in the world. The domestic per capita meat share reaches 58 kilograms, which is higher than the world average of 40 kilograms. With economic growth, food consumption has changed, and the consumption of livestock products has increased rapidly. This change has higher and more urgent requirements for the quality and safety of meat food. [0003] Among the known pathogenic bacteria, Salmonella (Salmonella spp.), Staphylococcus aureu (Staphylococcus aureu), Listeria monocytogenes (Listeria monocytogenes) often appear in all kinds of meat products, with high pathogenicity Morbidity and lethali...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12Q1/68C12Q1/14C12Q1/04
CPCY02A50/30
Inventor 余以刚肖性龙吴晖李苏龙刘园园
Owner SOUTH CHINA UNIV OF TECH
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