Duplex PCR detection kit and detection method for Listeria monocytogenes and Enterococcus faecium

A technology for Listeria and Enterococcus faecium, which is applied in biochemical equipment and methods, microorganism-based methods, and microbial determination/inspection to achieve the effects of convenient identification, strong specificity and high sensitivity
CN104846067AActive Publication Date: 2015-08-19INNER MONGOLIA AUTONOMOUS REGION ACAD OF AGRI & ANIMAL HUSBANDRY SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
INNER MONGOLIA AUTONOMOUS REGION ACAD OF AGRI & ANIMAL HUSBANDRY SCI
Publication Date
2015-08-19

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Abstract

The invention relates to a duplex PCR detection kit for Listeria monocytogenes and Enterococcus faecium and a detection method using the kit. The kit detects a primer pair of Listeria monocytogenes hlyA and a primer pair of the dd1 gene of an Enterococcus faecium strain; if specific amplification bands are obtained at 174 bp and 557 bp, Listeria monocytogenes and Enterococcus faecium are positive; and the kit can simultaneously detect Listeria monocytogenes and Enterococcus faecium. The lower limit of the detection sensitivity of the is lower than a minimum infective dose of pathogenic bacteria, which is of great significance to later clinical application and to monitoring of Listeria monocytogenes and Enterococcus faecium.
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Description

technical field

[0001] The invention relates to a kit for Listeria monocytogenes and Enterococcus faecium, which belongs to the field of animal epidemic molecular biology detection methods and detection reagents, and in particular to a double PCR for synchronously performing Listeria monocytogenes and Enterococcus faecium Detection kit and application kit Biological detection method for dual PCR detection of Listeria monocytogenes and Enterococcus faecium. Background technique

[0002] Listeria monocytogenes (Listeria monocytogenes) is a facultative intracellular parasite that can cause zoonotic listeriosis. The animal listeriosis it causes causes pigs, sheep, cattle, rabbits Meningoencephalitis, abortion, and acute sepsis occurred. Enterococci (Enterococci) belong to the normal flora in humans and animals, and are also important opportunistic pathogenic bacteria. Among the cases of infection caused by Enterococcus, the proportion of infection caused by Enterococcus faeciu...

Claims

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