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Listeria monocytogenes chromogenic medium and test sheet

A technology of Listeria monocytogenes and chromogenic medium, which is applied in the field of microbiological testing, can solve the problems of relying on new chromogenic reagents, complex synthesis procedures, high testing costs, etc., saving manpower and material resources, simple sterilization steps, The effect of simplifying the requirements for sterilization conditions

Inactive Publication Date: 2017-03-15
GUANGDONG DAYUAN OASIS FOOD SAFETY TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This scheme relies heavily on new color reagents, the synthesis process is relatively complicated, and the detection cost is relatively high

Method used

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  • Listeria monocytogenes chromogenic medium and test sheet
  • Listeria monocytogenes chromogenic medium and test sheet
  • Listeria monocytogenes chromogenic medium and test sheet

Examples

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preparation example Construction

[0025] The culture medium was prepared as follows:

[0026] 1) Dissolve the chromogenic agent in dimethylformamide, make a mother liquor and add it to the culture medium and mix evenly;

[0027] 2) Add water and stir the ingredients except the bacteriostatic agent until boiling, and keep boiling for 3-5 minutes after all raw materials are dissolved to sterilize;

[0028] 3) When the temperature of the culture medium drops to 40-50°C, add the bacteria inhibitor component and mix evenly, then pour the plate and prepare a corresponding chromogenic medium plate.

[0029] The preparation method of the test piece is as follows:

[0030] 1) Prepare the water-absorbing gel according to the proportion of the components of the water-absorbing gel, apply it evenly on the bottom plate, dehumidify and remove the water at 25°C, and make the bottom plate coated with the water-absorbing gel;

[0031] 2) Dissolve the chromogenic agent in dimethylformamide, make a mother solution, add it to t...

Embodiment 1

[0035] Each 1000mL medium contains 7g of peptone, 10g of yeast extract powder, 4g of sodium chloride, 3g of sodium pyruvate, 0.5g of lithium chloride, 3g of magnesium glycerophosphate, 0.02g of specific enzyme chromogenic substrate, and 200mg of bacteria inhibitors . The chromogenic substrate of the specific enzyme is 5-bromo-4-chloro-3-oxindole inositol phosphate sodium. Dissolve the above ingredients in 1000mL of water, add 12g of agar, heat and boil twice, wait for the culture medium to cool down to 40-50°C, add the mixed bacteria inhibitor mixture with a mass ratio of 1:4:6:3 ceftazidime, polymyxa sulfate Sodium, nalidixic acid, amphotericin, mix well and use.

Embodiment 2

[0037] Each 1000mL medium contains 25g of peptone, 4g of yeast extract powder, 15g of sodium chloride, 0.5g of sodium pyruvate, 5g of lithium chloride, 0.5g of magnesium glycerophosphate, 0.07g of specific enzyme chromogenic substrate, and bacteria inhibitors. 20 mg. The chromogenic substrate of the specific enzyme is 5-bromo-4-chloro-3-oxindole inositol potassium phosphate. Dissolve the above ingredients in 1000mL of water, add 15g of agar, heat and boil twice, wait for the culture medium to cool down to 40-50°C, add the mixed bacteria inhibitor mixture with a mass ratio of 1:1:2:1 ceftazidime, polymyxa sulfate Sodium, nalidixic acid, amphotericin, mix well and use.

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Abstract

The invention discloses a Listeria monocytogenes chromogenic medium and test sheet. The medium comprises peptone, yeast extract powder, sodium chloride, sodium pyruvate, lithium chloride, magnesium glycerophosphate, a specific enzyme chromogenic substrate and a competitor inhibitor. The chromogenic medium has the advantages of small use amount of the specific enzyme chromogenic substrate, great reduction of the cost, simple preparation method, simple sterilization steps, simplification of requirements of sterilization conditions, and high facilitation of use of base detection departments. The test sheet is convenient to use, and detection of Listeria monocytogenes can be completed after sample addition and constant temperature culture at 37 DEG C for 24-36 h, so a large amount of manpower and material resources can be saved.

Description

technical field [0001] The invention belongs to the field of microbiology testing, in particular to a chromogenic culture medium for detecting Listeria monocytogenes and a test piece made with the chromogenic medium. Background technique [0002] Listeria monocytogenes (Listeria monocytogenes) widely exists in nature and is a major pathogenic bacterium that can cause listeriosis in animals and humans. The infection mortality rate of pregnant women, infants, and immune tolerant patients is as high as 20% to 30%, which is much higher than that of other common foodborne pathogens. This bacterium is one of the four major food-borne disease-causing bacteria in the 1990s, and has been listed by the WHO as one of the key food-borne pathogens for detection. [0003] The national standard method for the detection of Listeria monocytogenes (GB / T 4789.30-2010) requires 7 days from sampling, enrichment, separation and purification with different culture media, and physiological and bio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04
CPCC12Q1/04
Inventor 范俊陈娟丽孙霞谢俊平卢新梁科
Owner GUANGDONG DAYUAN OASIS FOOD SAFETY TECH CO LTD
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