19 results about "Oxindole" patented technology

This invention discloses an oxyindene compound containing substituted pyridine and its application as a fungicide. The oxyindene compound containing substituted pyridine has broad-spectrum fungicidal activity, especially effective in controlling rice sheath blight, apple tree rot, rapeseed sclerotinia stem rot, wheat scab, pepper blight, cucumber anthracnose, tomato gray mold, corn curvature blight, rice blast, tomato early blight, wheat take-all, and wheat stem rot. It can be used as a fungicide for the prevention and control of the above-mentioned plant diseases.

This invention discloses a fluorine-containing unsaturated hydroxyindole compound, its preparation method, and its applications. Using (E)-3-nitromethylene hydroxyindole as the key synthon, it is synthesized via S... N A series of novel hydroxyindole derivatives were designed and constructed using the V-reaction, and their antibacterial activities were explored. The method for constructing fluorinated unsaturated hydroxyindole compounds obtained by this invention is of positive significance for promoting the synthesis and widespread application research of hydroxyindole compounds.

The present invention is directed to the Senantiomer of spiro-oxindole of formula (I), and pharmaceutical composition containing the compound which may be useful in treating diseases or conditions in a mammal, preferably human, which are ameliorated or alleviated by the inhibition of voltage-gated sodium channels.

The invention discloses a synthetic method of a 3-spirobutyl oxoindole compound containing trifluoromethyl, and belongs to the technical field of organic synthesis. According to the invention, under the action of visible light induction, a photocatalyst and alkali, CF3Br and N-aryl bicyclo [1.1. 0] butane-1-formamide are subjected to a free radical tandem cyclization reaction in an organic solvent, so that the 3-spirobutyl oxoindole compound containing trifluoromethyl is prepared. According to the invention, free radical series cyclization reaction of CF3Br and saturated carbon atoms, namely sp3 hybrid carbon atoms, is realized for the first time, and the variety of organic matters reacting with CF3Br is enriched. The preparation method provided by the invention provides a new path for synthesis of 3-spirobutyl oxindole containing trifluoromethyl, has the advantages of cheap and easily available raw materials, mild reaction conditions, simple operation, high product yield and the like, and accords with the concept of green synthesis.

The invention provides an ion solvated membrane and a preparation method and application thereof.The ion solvated membrane comprises at least one of branched polyaryloxyindole and side-chain polyaryloxyindole, the branched polyaryloxyindole comprises at least one of a compound A1, a compound A2 and a compound A3, and the side-chain polyaryloxyindole comprises at least one of side-chain polyaryloxyindole and side-chain polyaryloxyindole. The structure of the side-chain type polyarylene oxoindole is as shown in a compound B; the compound A1 comprises a plurality of first chain segments and a plurality of second chain segments, the compound A2 comprises a plurality of first chain segments and a plurality of third chain segments, the compound A3 comprises a plurality of first chain segments, a plurality of second chain segments and a plurality of third chain segments, a is the number of side chain carbon atoms, and M is an aromatic unit; ar1 is a monomer having a first number of branching points, Ar2 is a monomer having a second number of branching points, and M is different from Ar1 and Ar2. The membrane effectively solves the problems of short operation life and poorer electrochemical performance of the existing ion solvation membrane.

The present application relates to the technical fields of medical and pharmaceutical chemical synthesis, and particularly relates to a synthesis method of 3-thiocyanatospiro cyclobutyl oxindole compounds, which comprises the following steps: in a reactor, compound 1, compound 2, a catalyst, an additive and a solvent are stirred for 30 minutes at room temperature, after the reaction is completed, the crude product is obtained by reduced pressure distillation, and the 3-thiocyanatospiro cyclobutyl oxindole compound is obtained by column chromatography and thin layer chromatography purification. N The present application takes phenyl bicyclo[1.1.0]butane-1-formamide compounds and thiocyanate as raw materials, and promotes the synthesis of 3-thiocyanatospiro cyclobutyl oxindole compounds by an oxidant. The present application has the advantages of simple synthesis steps, mild conditions, safe operation, good functional group compatibility, high reaction conversion, low cost and the like, and is conducive to industrial production.

The application discloses a preparation method of ultraviolet absorber and sunscreen, and belongs to the cosmetic field. The preparation method of the ultraviolet absorber comprises the following steps: dissolving 5,6-dihydroxyindole-2-carboxylic acid in a solvent to obtain a 5,6-dihydroxyindole-2-carboxylic acid solution; adding 2-formylphenylboronic acid and amino-terminated polydimethylsiloxane into the 5,6-dihydroxyindole-2-carboxylic acid solution, and stirring to obtain a mixed solution; and removing the solvent in the mixed solution to obtain the ultraviolet absorber. The preparation method of the sunscreen comprises the following steps: adding the ultraviolet absorber and zinc oxide nanoparticles into an oil phase in a moisturizing cream base, performing colloidal grinding, and performing heating and stirring until emulsification is achieved; and then mixing a water phase in the moisturizing cream base to obtain the sunscreen. The application can solve the problems that 5,6-dihydroxyindole-2-carboxylic acid has the shortcomings of narrow ultraviolet absorption band, easy oxidation polymerization leading to color deepening, and inability to realize molecular-level synergistic regulation of electronic structure to realize directional red shift of the absorption band.

The invention belongs to the technical field of tumor drug research, and particularly relates to application of 2-phenyl oxindole in preparation of a drug for reversing cis-platinum drug resistance or treating tumors. In-vitro experiments and in-vivo experiments prove that the 2-phenyl oxoindole can effectively inhibit proliferation of triple negative breast cancer cells and can remarkably improve the sensitivity of the cells to cis-platinum with a series of concentrations, and pathological staining results of visceral organs and tissues of mice show that the 2-phenyl oxoindole can effectively inhibit proliferation of the triple negative breast cancer cells and remarkably improve the sensitivity of the cells to cis-platinum with a series of concentrations. The 2-phenyl oxoindole does not cause obvious injury to main organs such as liver, kidney and the like.

This invention relates to a method for producing indirubin using a co-culture system of *E. coli*, comprising the following steps: Step 1, culturing a first engineered bacterium to the late logarithmic growth stage to obtain a fermentation broth of the first engineered bacterium; Step 2, activating and culturing a second engineered bacterium to the early logarithmic growth stage, collecting and resuspending the bacterial cells to obtain a bacterial suspension of the second engineered bacterium; Step 3, inoculating the bacterial suspension of the second engineered bacterium into the fermentation broth of the first engineered bacterium, adding cysteine ​​to begin co-culture, and adding an inducer to induce expression; when co-culturing for 16-30 hours, adding indigo and / or 2-hydroxyindole; continuing co-culturing for 40-60 hours to obtain a fermentation broth containing indirubin. This invention, through a staged culture-mixed co-culture strategy, effectively solves key technical bottlenecks such as heavy metabolic burden of single strains, high toxicity of the intermediate product indole, and high production of the byproduct indigo, achieving a shake-flask level yield of 163.34 mg / L of indirubin, demonstrating good prospects for industrial application.

The invention relates to a polyaryl-substituted methylene oxoindole compound, a synthesis method thereof and application of the compound in preparation of antitumor drugs. The chemical structural formula of the compound is as shown in formula 3. The synthesis method comprises the following steps: adding a compound oxoindole derivative o-amino styrene shown as a formula 1 and a compound 1, 4-diketone derivative shown as a formula 2, which serve as reaction raw materials, into carbon tetrachloride, adding a dehydrating agent, stirring and reacting at room temperature for 12 hours under the catalysis of Bronsted acid of a compound shown as a formula 4, tracking the reaction by TLC until the reaction is complete, filtering, concentrating and purifying, thereby obtaining the 2-oxoindole derivative o-amino styrene. According to the present invention, the antitumor activity test results show that the synthesized polyaryl-substituted methylene oxoindole compound has high sensitivity and strong cytotoxic activity on human nasopharyngeal carcinoma cells ONE-1; the reaction conditions are relatively conventional, the reaction process is mild, simple and convenient, the cost is low, and the application range of the method is widened; various substrates are used as reactants, various products are obtained, and the yield is high.

The invention discloses a high-temperature-resistant and high-concentration alkali-resistant ion solvated microporous membrane and a preparation method and application thereof, and relates to the technical field of separation membranes. Uniformly distributed microporous structures are introduced into a polymer membrane through a controllable super-crosslinking reaction, so that an ion transmission channel is effectively expanded, and the ion flux of the membrane is remarkably improved; meanwhile, stable oxindole / OH <-> complex ion pairs are constructed in situ, and a continuous ion solvation micropore channel is formed; the channel not only provides abundant transmission sites for OH, but also constructs a selective transmission path penetrating through the membrane thickness direction, so that excellent ion selectivity and chemical stability can still be maintained under the conditions of high-concentration alkali liquor and high temperature.

The invention belongs to the technical field of medicines, and relates to four spirooxindole alkaloids extracted and separated from persicaria tinctoria as well as a preparation method and application of the four spirooxindole alkaloids. Compounds 1a / 1b are a pair of enantiomers having a 6 / 5 / 5 / 6 tetracyclic system and a 3-phenyl-4, 5-dihydro-3H-spiro [furan-2, 3 '-indoline] skeleton. The compound 2 and the compound 3 have a 6 / 5 / 6 / 5 / 6 pentacyclic system skeleton and a 3 '-methyl-2', 3 ', 4', 9 '-tetrahydrospiro [indole-3, 1'-pyrido [3, 4-b] indole] skeleton. The invention also relates to a separation and biomimetic synthesis preparation method of the compound, and application of the compound in preparation of drugs for treating neurodegenerative diseases. Reagents needed by the preparation method are low in price and convenient and easy to obtain, and the obtained compound has a good neuroprotective effect, has the advantages of being easy and convenient to operate and good in reproducibility, is beneficial to industrial production and has high potential value and wide application prospects in the fields of medicine and medicinal chemistry and synthesis of natural products.

The application discloses a green and environment-friendly reaction of visible light-induced alpha, beta-unsaturated amide and aryl triazene in air without any additive or cosolvent, so that bifunctionalization is realized, and when the condition is changed and when there is a protective group on N, a cyclization reaction can also be realized. The metal-free reaction has high chemical selectivity, and corresponding beta-hydroxyl compounds and competitive cyclization products can be synthesized. The reaction has considerable advantages, including high selectivity, mild reaction condition, cleanness, no need of transition metal catalyst, high atom economy and good functional group compatibility.

The present invention provides stereogenic 3,3-disubstituted 3-aminooxindole compounds of formula (I). In addition, the present invention provides a process for preparing a stereogenic 3,3-disubstituted 3-aminooxindole compound of formula (I) using isatins and N-methoxy benzamides in presence of highly abundant iron catalyst. The stereogenic 3,3-disubstituted 3-aminooxindole compound of formula (I) is useful in the preparation of various bio-relevant C3-aminated oxindoles.

A biosynthetic method and composition for producing an Indigo dyestuff including Indigotin and Indirubin. Tryptophanase (TRP) and flavin-containing monooxygenase (FMO) are generated from a genetically-modified organism having a vector coding for tryptophanase and flavin-containing monooxygenase. The conversion of L-tryptophan to Indigo dyestuff is converted by the generated TRP and FMO in the presence of one or more bioactive additives selected from the cysteine, 2-oxindole, or 2-indoxyl to form a dyestuff having a ratio of Indigotin to Indirubin by weight between 1:0.1 to 1:4. The dyestuff has a color hue defined by CIE XYZ color space with X: 0.1920 to 0.2481, Y: 0.2537 to 0.2019, Z: 0.5543 to 0.5501. Selectable and reproduceable color hues are provided.

The invention discloses a preparation method of a poly (oxindole biphenyl) microfiltration membrane, the microfiltration membrane and application of the microfiltration membrane in bacterial separation. The preparation method comprises the following steps: dissolving a poly (oxindole biphenyl) polymer in an aprotic solvent to obtain a homogeneous solution, adding the aprotic solvent into the homogeneous solution, and adding a plasticizer to obtain a membrane casting solution; uniformly casting the membrane casting solution on a substrate on a casting machine to obtain a membrane casting sheet; the casting membrane is placed in a gas phase environment for phase separation, and the relative humidity of the gas phase environment is controlled to range from 40% to 100%; the temperature is controlled to be more than 20 DEG C and less than 60 DEG C, and the retention time is 0-10 minutes; and after phase inversion is completed, immersing the casting membrane sheet into a non-solvent coagulating bath for coagulating, and washing and drying to obtain the poly (oxindole biphenyl) microfiltration membrane. The method has the advantages that repeated sterilization circulation can be tolerated, the stability of a pore structure is kept, and the intrinsic anti-pollution characteristic is achieved.

The invention discloses a 3-position polysubstituted chiral oxindole derivative as well as a synthesis method and application thereof. According to the synthesis method of the 3-position polysubstituted chiral oxoindole derivative, organic alcohol, isatin diazo and allyl carbonate are used as raw materials, chiral phosphoric acid is used as a chiral inducer, an organic reagent is used as a solvent, and the chiral oxoindole derivative is obtained through a one-step reaction under the catalysis of palladium-rhodium bimetal. The raw materials are cheap, easy to obtain, safe and non-toxic, the reaction conditions of the synthesis method are mild, the operation is simple and safe, and the synthesized 3-position polysubstituted chiral oxindole derivative can be widely applied to the fields of organic synthesis and drug research and development.

The application discloses a preparation method of 5-cyanoindole, S1) o-chloroaniline and dimethyl malonate are dissolved and subjected to catalytic reaction to prepare dimethyl 2-(2-aminophenyl)malonate; S2) dimethyl 2-(2-aminophenyl)malonate is dissolved, hydrolysis reaction is carried out by adding alkali, and then high-temperature decarboxylation reaction is carried out by adding acid to prepare o-aminophenylacetic acid; S3) o-aminophenylacetic acid is dissolved and subjected to amino para-bromination to prepare 2-(2-amino-5-bromophenyl)acetic acid; S4) 2-(2-amino-5-bromophenyl)acetic acid is dissolved, and intramolecular condensation is carried out by adding a condensing agent to prepare 5-bromo-oxindole; S5) 5-bromo-oxindole is subjected to reduction reaction to prepare 5-bromoindoline; S6) 5-bromoindoline is subjected to oxidation reaction to prepare 5-bromoindole; and S7) substitution reaction is carried out on the bromine of 5-bromoindole by using cyano to prepare 5-cyanoindole. The application has the advantages of simple process, environmental protection, no pollution, low cost, safe operation, easy scale production, mild reaction condition, high conversion rate and good product quality.