Annular mediated isothermal amplification-nano cadmium sulphide mark electrochemistry checking method for Listeria monocytogenes

Abstract

The invention relates to a method for detection Listeria monocytogenes with loop-mediated isothermal amplification-CdS nanoparticles labeling electrochemistry. The method is characterized in that the method comprises the following steps of: obtaining double-stranded target sequence of amplification reagent-Listeria monocytogenes to be detected by means of loop-mediated isothermal amplification reaction; pyrolyzing a double-stranded target sequence in water bath into a single-stranded target sequence, then self assembling and fixing at the surface of gold electrode, so that the target sequence modified electrode of the Listeria monocytogenes to be detected is obtained; afterwards, labeling the probe sequence coming from the Listeria monocytogenes by CdS nanoparticles to obtain a labeled probe sequence; carrying out molecular hybridization on the labeled probe sequence and the target sequence of the target sequence modified electrode, and carrying out electrochemical detection by simultaneity plating Hg anodic stripping method; detecting out cadmium ions corresponding to the species-specific genes (actA) of the detected Listeria monocytogenes. The method has the advantages of speediness, strong specificity, high sensitivity and convenient use.

Description

technical field

[0001] The invention relates to a rapid gene detection method, specifically a method for detecting the genus-specific gene (actA) of Listeria monocytogenes by combining loop-mediated isothermal amplification (LAMP) technology and nano-label electrochemical DNA sensing technology ——Ring-mediated isothermal amplification-a method for the electrochemical detection of Listeria monocytogenes with nano-cadmium sulfide labeling. Background technique

[0002] Listeria monocytohenes is a pathogen widely distributed in nature. It exists in soil, surface water, sewage, waste water, plants, silage, and rotten vegetables, so it is easy for animals to ingest it. The bacteria are transmitted through the oral-fecal route. According to reports, the carrying rate of Listeria monocytogenes in the feces of healthy people is 0.6-16%, 70% of people can carry bacteria for a short time, 4-8% of aquatic products, 5-10% of milk and its products, 30% More than 15% of meat products an...