Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Coxsackie A16 type virus mutant strain capable of effectively infecting mice

A technology of A16 and mutant strains, applied in the field of virology, can solve the problem of little research on CA16 mouse infection models

Active Publication Date: 2014-06-04
INST PASTEUR OF SHANGHAI CHINESE ACADEMY OF SCI
View PDF1 Cites 13 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The mouse infection model is an essential tool for evaluating candidate vaccines. There have been many reports on the EV71 mouse infection model, mainly to obtain mutant strains in suckling mice. Little research has been done on the CA16 mouse infection model

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Coxsackie A16 type virus mutant strain capable of effectively infecting mice
  • Coxsackie A16 type virus mutant strain capable of effectively infecting mice
  • Coxsackie A16 type virus mutant strain capable of effectively infecting mice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] Example 1, mouse-adapted strain G08-MAV has higher toxicity to mouse cells

[0083] Vero cells were infected with CA16 (MOI=0.01) wild strain G08 or mouse-adapted strain G08-MAV, and both G08 and G08-MAV caused severe lesions in Vero cells after 24 hours ( figure 1 A, 1D); CA16 (MOI=0.01) G08 or mouse-adapted strain G08-MAV infected mouse cells L929, and G08-MAV infected L929 cells for 24 hours, and severe lesions occurred ( figure 1 E), all lesions after 48h ( figure 1 F), while G08 infected L929 cells 24h and 48h without lesion ( figure 1 B, 1C). In order to further compare the toxicity of G08 and G08-MAV on cells, plaque experiments were performed on Vero and L929 cells.

[0084] The results showed that the plaque size of G08 and G08-MAV in Vero was not much different ( figure 2 A, 2C), the plaques of G08-MAV on L929 were significantly larger than those of G08 ( figure 2 D, 2B), indicating that the mouse-adapted G08-MAV has significantly enhanced toxicity on m...

Embodiment 2

[0085] Example 2. The pathogenicity of G08-MAV in mice is significantly enhanced

[0086] Use 1.35×10 6 G08 of TCID50 and 1.35×10 4 The TCID50 mouse-adapted strain G08-MAV was injected intraperitoneally into 7-day-old ICR mice, and the mortality rates of the mice were 26.7% and 71% ( image 3 B), 7-day-old mice died 6 days after infection with G08-MAV, and 7-day-old mice infected with G08 died after 9 days. After infection, the mice showed clinical symptoms of slow response, ataxia, leg weakness followed by paralysis and even death ( image 3 A, 3C), some mice died without paralysis after infection, and the mice either continued to lose weight two to three days before death, or gradually lost weight accompanied by paralysis of at least two legs. The clinical symptoms of G08-MAV-infected mice were significantly more severe than those of G08-infected mice ( image 3 C).

[0087] Animal infection experiments showed that mouse-adapted virus G08-MAV could cause more serious le...

Embodiment 3

[0088] Example 3, intraperitoneal injection of G08-MAV can effectively infect 2-week-old ICR mice

[0089] 1.35×10 4 TCID50 mouse-adapted strain G08-MAV intraperitoneally infected 2-day-old, 5-day-old, 10-day-old, 14-day-old and 21-day-old ICR mice. and 10-day-old mice observed for 14 days after infection showed approximately the same mortality rates of 79% and 80%, respectively, 14-day-old mice had a 50% mortality rate, and 21-day-old mice did not die after infection ( Figure 4 A), as the age increases, the lethality of mouse-adapted virus to mice shows a downward trend. The younger the infected mice are, the earlier they die, and the 2-day-old mice appear 4 days after infection. die( Figure 4 A). Mice aged two weeks and below showed clinical symptoms of slow response, ataxia, leg weakness and then gradual emaciation, paralysis and even death after infection ( Figure 4 B).

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a Coxsackie A16 type virus mutant strain capable of effectively infecting mice. In the invention, a mouse-adaptive mutant virus strain is unexpectedly obtained through continuous passage of CA16 virus by using the mouse cells and mouse; the mouse-adaptive strain has very strong mouse infection ability and can be used for preparing a mouse model of Coxsackie virus infection so as to study the pathogenic mechanism of the virus, evaluate the antiviral drugs, etc.

Description

technical field [0001] The invention belongs to the field of virology; more specifically, the invention relates to a Coxsackie A16 virus mutant strain capable of effectively infecting mice. Background technique [0002] Hand, Foot and Mouth Disease (HFMD) is a common viral disease in children under the age of 5. The affected children manifest as fever, oral ulcers, skin rashes on the hands and feet, and severe respiratory symptoms. , circulatory and nervous system lesions and even death, Coxsackievirus (Coxsackievirus A16, CA16) and enterovirus 71 (Enterovirus71, EV71) are the two main pathogens of HFMD. [0003] The recent outbreak of hand, foot and mouth disease in China is related to both CA16 and EV71. The prevalence of CA16 and EV71 varies greatly in different regions. In 2009, 61.9% of HFMD patients in Shandong were infected with EV71, and 16.5% were infected with CA16. %, however, EV71 and CA16 infection accounted for 38.3% and 58.5% of HFMD patients in Guangdong in ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N7/00A01K67/027A61K49/00C12R1/93
Inventor 黄忠刘庆伟
Owner INST PASTEUR OF SHANGHAI CHINESE ACADEMY OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com