ApoA5 genic mutation detection specific primer and liquid phase chip

Abstract

The invention discloses an apoA5 genic mutation detection specific primer and a liquid phase chip, wherein the liquid phase chip mainly includes an ASPE primer formed by 5'end tag sequence and 3'end specific primers, and the specific primers are SEQ ID NO.13 and SEQ ID NO.14 for the mutation of A134G, SEQ ID NO.15 and SEQ ID NO.16 for the mutation of T63C, SEQ ID NO.17 and SEQ ID NO.18 for the mutation of C41G, SEQ ID NO.19 and SEQ ID NO.20 for the mutation of G54T, SEQ ID NO.21 and SEQ ID NO.22 for the mutation of A81G, and / or SEQ ID NO.23 and SEQ ID NO.24 for the mutation of G194A; a microsphere enveloped by anti-tag sequence; and an amplimer. The matching degree of the detection result of the apoA5 genic mutation detection liquid phase chip and the sequencing method is 100%, and the parallel detection of multiple polymorphic sites can be achieved.

Description

Technical field [0001] The invention is subservient to the field of molecular biology, and relates to medicine and biotechnology, and specifically relates to a specific primer for detecting apoA5 gene and a liquid phase chip. Background technique [0002] Apolipoprotein A5 (apolipoprotein A5 gene, apoA5), a member of the apolipoprotein family, is located in the q23 region (11q23) of the long arm of chromosome 11, about 30 kb away from the apoA1 / C3 / A4 gene cluster, and the gene is 1889 bp in length This gene has 4 exons, 2 introns and 4 silencers, which encodes a protein composed of 363 amino acids, which has an important influence on plasma triglyceride (TG) levels. The relationship between plasma TG metabolism and cardiovascular disease is very close. At present, NCBI has included more than 20 SNP sites. The target detection of apoA5 gene mutation sites in the present invention is shown in the table: [0003] Serial number The content of apoA5 site mutation Shorthand 1 ...

AI Technical Summary

Problems solved by technology

The PCR-RFLP method is based on the change of the restriction endonuclease recognition site caused by gene mutation, such as the loss of the site or the generation of a new site, a specific fragment is amplified by PCR, and then cut and amplified with a restriction endonuclease. Amplify the product and observe the size of the fragment by electrophoresis. This method is used to detect gene mutations with altered restriction sites, and can directly determine the genotype. However, this method cannot be used for the detection of gene mutations without new restriction sites.

Again, these methods all have limitations in detection throughput, and can only detect one mutation type at a time, which cannot meet the needs of practical applications.

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