ATM gene mutation detection specific primer and liquid chip

Abstract

The invention discloses an ATM gene mutation detection specific primer and a liquid chip. The liquid chip mainly comprises ASPE primers consisting of tag sequences at a 5' end and specific primer sequences aiming at target gene mutation sites at a 3' end, microspheres coated with anti-tag sequences and an amplification primer, wherein the specific primer sequences are as follows: SEQ ID NO.13 and SEQ ID NO.14 which aim at a T2572C site, SEQ ID NO.15 and SEQ ID NO.16 which aim at an A7325C site, SEQ ID NO.17 and SEQ ID NO.18 which aim at a G7328A site, SEQ ID NO.19 and SEQ ID NO.20 which aim at a C9022T site, SEQ ID NO.21 and SEQ ID NO.22 which aim at a G9023A site, and / or SEQ ID NO.23 and SEQ ID NO.24 which aim at a C9139T site. The detection liquid chip provided by the invention has the advantages that the matching rate between the detection result and a sequencing method is up to 100%, and the wild-type and mutant parallel detection of multiple mutant sites can be realized.

Description

technical field [0001] The invention belongs to the field of molecular biology, relates to medicine and biotechnology, in particular to a specific primer for ATM gene mutation detection and a liquid phase chip. Background technique [0002] Ataxia-telangiectasia mutated (ATM) is the causative gene that causes ataxia telangiectasia. ATM gene is an important signal sensor. In the process of DNA damage repair, As a recognition factor, ATM detects damaged DNA and repairs DNA double-strand breaks by phosphorylating target proteins. [0003] The ATM gene is located on chromosome 11q22.3, with a length of 150kb and 66 exons, and the fourth exon is the first coding exon. ATM protein is a phosphorylated macromolecular nucleophosmin protein encoded by the ATM gene, containing 3056 amino acids. Studies have shown that ATM gene mutation is closely related to the occurrence of tumors. [0004] At present, there are few methods for detecting and analyzing ATM gene mutations, mainly inc...

AI Technical Summary

Problems solved by technology

The PCR-RFLP method is based on the change of the restriction endonuclease recognition site caused by gene mutation, such as the loss of the site or the generation of a new site, a specific fragment is amplified by PCR, and then cut and amplified with a restriction endonuclease. Amplify the product and observe the size of the fragment by electrophoresis. This method is used to detect gene mutations with altered restriction sites, and can directly determine the genotype. However, this method cannot be used for the detection of gene mutations without new restriction sites.

Again, the above methods all have limitations in detection throughput, and can only detect one mutation type at a time, which cannot meet the needs of practical applications.

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