Primer set and kit for detecting polymorphism of hyperglycemia drug metabolism related genes and application of primer set and kit

A technology of gene polymorphism and polymorphism, which is applied in the fields of medicine and biology, can solve problems such as aggravating adverse reactions and inability to effectively lower blood sugar, and achieves the advantages of reducing adverse drug reactions, high sensitivity and specificity, and simple sample processing Effect

Pending Publication Date: 2021-01-12
PRECEDO PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current treatment method of "one drug for a thousand people" cannot effectively lower blood sugar, and will aggravate adverse reactions

Method used

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  • Primer set and kit for detecting polymorphism of hyperglycemia drug metabolism related genes and application of primer set and kit
  • Primer set and kit for detecting polymorphism of hyperglycemia drug metabolism related genes and application of primer set and kit
  • Primer set and kit for detecting polymorphism of hyperglycemia drug metabolism related genes and application of primer set and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] This example is used to illustrate the selection of the target gene

[0070] (1) Referring to the "Technical Guidelines for the Detection of Drug Metabolizing Enzymes and Drug Target Genes" and the FDA's clear pharmacogenomics-related biomarkers, combined with a large number of clinical research reports, the results that are closely related to the efficacy of oral hypoglycemic drugs are summarized Genes and mutation sites;

[0071] (2) After signing the informed consent form, collect samples from patients with hyperglycemia (use a cotton swab to dip in the oral mucosal cells), and use the kit to extract the DNA in the oral mucosal cell samples dipped in the cotton swab;

[0072] (3) Detect the mutation sites of the genes involved in step 1. Taking the gene CYP2C9 as an example, using the extracted human oral cell DNA as a template, carry out PCR on-machine amplification with the corresponding upstream and downstream primers (sequence 1, 2). Increase, purify the PCR pro...

Embodiment 2

[0078] This embodiment is used to illustrate the composition and use of the kit of the present invention

[0079] 1. The composition of the kit

[0080] (1) Primers for genes related to hyperglycemia drug metabolism:

[0081] Entrust Suzhou Jinweizhi Biotechnology Co., Ltd. to synthesize the amplification primers and sequencing primers in Table 2.

[0082] (2) Selection of reference substances:

[0083] Human genomic DNA was used as a positive quality control; sterilized distilled water was used as a negative control.

[0084] (3) PCR reaction solution:

[0085] Prime HS (Premix)

[0086] 2. Use of the kit

[0087] (1) Obtaining samples

[0088] A DNA extraction kit was used to extract DNA from the oral mucosal cell samples taken from the subjects' cotton swabs.

[0089] (2) Sample testing

[0090] Take out the relevant reagents and prepare the PCR system according to Table 3.

[0091] table 3

[0092]

[0093] The PCR reaction program of this system is shown i...

Embodiment 3

[0111] This example is used to illustrate the effect of the primer set and / or kit of the present invention on drug guidance for patients with hyperglycemia

[0112] In order to verify the effect of the primer set and / or kit of the present invention on the medication guidance of patients with hyperglycemia, volunteers with hyperglycemia patients were selected in the "Present Medical Laboratory" and signed informed consent, using the invented primer set and / or the kit performs genotype detection according to the system and conditions of Example 2, accurately formulates its medication plan according to the test results of each volunteer, and conducts clinical follow-up to observe the clinical medication effect.

[0113] Medication Guidance Example 1

[0114] The tester, Li, had unsatisfactory hypoglycemic effect after taking troglitazone before the test. He used the primer set and / or kit of the present invention to detect hyperglycemia-related genes. The test results are shown i...

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Abstract

The invention relates to the technical field of medicine and biology, and discloses a primer set and a kit for detecting polymorphism of hyperglycemia drug metabolism related genes and application ofthe primer set and the kit. The primer set comprises primers capable of amplifying at least one hyperglycemia drug metabolism related gene: CYP2C9, KCNJ11, PPARgamma, SLCO1B1, SLC22A1, SLC22A2, and APOE. The primer set can also comprise a sequencing primer for carrying out Sanger sequencing on at least one of the above genes. The primer set and kit can formulate different schemes for hyperglycemiapatients according to individual differences of genes, select appropriate hypoglycemic drugs, realize accurate typing and precise medication, thereby increase hypoglycemic effect and reduce adverse drug reactions. The preferred primer set has high sensitivity and specificity, and when the preferred primer set is used for genotyping genes related to hyperglycemia drug metabolism, the primer set has advantages of being accurate in qualitative analysis, high in specificity and the like.

Description

technical field [0001] The invention relates to the fields of medicine and biotechnology, in particular to a primer set and a kit for detecting hyperglycemic drug metabolism-related gene polymorphisms and applications thereof. Background technique [0002] China is the country with the largest number of diabetic patients in the world. According to the latest diabetes map released by the International Diabetes Federation (IDF) in 2017, the number of diabetics in China has reached 114 million, ranking first in the world, and type 2 diabetes accounts for more than 90% of diabetic patients. Diabetes is a metabolic disease characterized by hyperglycemia in the blood. Hyperglycemia is due to defective insulin secretion or impaired biological function. If the human body has high blood sugar for a long time, it will lead to chronic damage and dysfunction of various tissues, especially the eyes, kidneys, heart, blood vessels, and nerves. [0003] There are two main reasons for diabe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/156C12Q2600/106
Inventor 周玲燕张定芳黄瑞骆侠郭冬生
Owner PRECEDO PHARMA CO LTD
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