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Detection of curative effect of anti-angina pectoris drug

Inactive Publication Date: 2010-12-29
XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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  • Summary
  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

The main way of gene mutation is that base deletion or substitution causes the reading frame to shift, or the loss of large fragments of genes

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0026] Example Use of detection kit

[0027] Step 1: Extraction of DNA template

[0028] The genomic DNA of oral epithelial cells was extracted by silica gel adsorption method.

[0029] Step 2: Fluorescence quantitative PCR reaction

[0030] Use the fluorescent quantitative PCR kit in the detection kit to perform 5 independent fluorescent quantitative PCR reactions, each reaction system is a total volume of 10μl, containing a concentration of 20ng / μl DNA template 2μl, 1μl 10X fluorescent quantitative PCR reaction buffer , 0.1μl 25mM dNTP mixture, 0.6μl 25mM MgCl 2 Solution, 0.025μl (5units / μl) Taq DNA polymerase, 20μM sense primer and antisense primer 0.225μl, 10μM fluorescent probe with VIC and 0.25μl with FAM fluorescent probe, deionized water 5.325μl.

[0031] The reaction was carried out on a PCR amplification machine, and the reaction conditions were 50°C, 2 minutes, 95°C, 10 minutes, and 60 cycles of 95°C, 30 seconds, 60°C, and 1 minute. After the reaction is over, the fluoresce...

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PUM

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Abstract

The invention discloses a kit for detecting the curative effect of an individual anti-angina pectoris drug. The kit comprises a specific primer pair and a specific fluorescent probe pair for detecting the polymorphic sites of an ALDH2 gene, a CYP3A4 gene, a CYP3A5 gene, a CYP2D6 gene and a CYP2C9 gene at the targeted use sites of the anti-angina pectoris drug, fluorescent quantitative PCR conventional components, etc. The kit estimates the curative effect of the individual anti-angina pectoris drug by detecting the polymorphic sites of the ALDH2 gene, the CYP3A4 gene, the CYP3A5 gene, the CYP2D6 gene and the CYP2C9 gene at the targeted use sites of the anti-angina pectoris drug.

Description

Technical field [0001] The present invention relates to the fields of molecular biology and medicine. More specifically, the present invention relates to a kit for detecting the efficacy of anti-angina pectoris drugs in individuals, by detecting the ALDH2 gene, CYP3A4 gene, CYP3A5 gene, and CYP2D6 gene at targeted sites of antiangina pectoris drugs. And CYP2C9 gene polymorphism sites to evaluate the efficacy of individual anti-angina drugs. Background technique [0002] Angina pectoris (angina pectoris) is a clinical syndrome caused by insufficient coronary blood supply, acute myocardial ischemia, and hypoxia. It is characterized by paroxysmal squeezing pain in the front chest, which may be accompanied by other symptoms. The pain is mainly located at the back of the sternum and can radiate to the precordial area and the left upper limb. It often occurs during labor or emotional excitement and lasts for several minutes. It disappeared after resting or using nitrate preparations. ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N21/64
Inventor 冯哲民邹祖烨
Owner XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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