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Kit for detecting human diabetes sensitive genes

A technology of diabetes and kits, which is applied in the field of biomedicine, can solve the problems of high detection cost and low detection throughput, and achieve the effect of high throughput, low cost and rapid detection

Inactive Publication Date: 2021-01-22
为康(苏州)基因科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the drug metabolism gene detection technology of diabetes in the market is mostly carried out by fluorescent PCR and other technologies; the detection throughput is small and the detection cost is high

Method used

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  • Kit for detecting human diabetes sensitive genes
  • Kit for detecting human diabetes sensitive genes
  • Kit for detecting human diabetes sensitive genes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] In this example, C11orf 65, CYP2C9, TCF7L2, ABCC8, and KCNJ11, the mutant gene sites of human diabetes drug sensitive genes, are used as detection objects, and through specific primer combinations and nucleic acid mass spectrometry techniques, accurate, simple, and rapid simultaneous detection of C11orf 65 can be achieved. , CYP2C9, TCF7L2, ABCC8, KCNJ11 five gene loci.

[0062] The gene template of the experiment is: 30 wild-type blood samples and 5 mutant blood samples, which are tested at the same time, and finally the correct rate of negative and positive is used as the standard.

[0063] The first step, multiplex PCR reaction:

[0064] Primer set a mix (including primers C11orf 65F1, C11orf 65R1, CYP2C9F1, CYP2C9R1, TCF7L2F1, TCF7L2R1, ABCC8F1, ABCC8R1, KCNJ11F1, KCNJ11R1) 1ul

[0065] PCR master Mix 1.2ul

[0066] H2O 0.8ul

[0067] DNA template 2ul

[0068] The reaction conditions of the multiplex PCR reaction are: 95° C. for 2 minutes, 95° C. for 30 seconds,...

Embodiment 2

[0077] In this example, C11orf 65, CYP2C9, TCF7L2, ABCC8, and KCNJ11, the mutant gene sites of human diabetes drug sensitive genes, are used as detection objects, and through specific primer combinations and nucleic acid mass spectrometry techniques, accurate, simple, and rapid simultaneous detection of C11orf 65 can be achieved. , CYP2C9, TCF7L2, ABCC8, KCNJ11 five gene loci.

[0078] The gene template of the experiment is: 1 wild-type blood sample and 1 mutant blood sample, and 20 blood samples and 10 saliva samples are tested simultaneously.

[0079] The method for using the above-mentioned multiplex PCR to detect the mutation gene site of the human diabetes drug sensitive gene comprises the following steps:

[0080] (1) Sample processing and template extraction quality control:

[0081] The scope of application of samples includes whole blood, saliva, oral test strips and other specimens; for the extraction process, please refer to the instructions of the relevant finishe...

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Abstract

The present invention discloses a kit for detecting human diabetes sensitive genes. The kit comprises a C11orf 65 primer group, a CYP2C9 primer group, a TCF7L2 primer group, an ABCC8 primer group anda KCNJ11 primer group. The human diabetes drug sensitive genes C11orf 65, CYP2C9, TCF7L2, ABCC8 and KCNJ11 are used as detection objects, and variations of the related genes can be rapidly, simply andaccurately detected by combining the specific primers and combining a nucleic acid mass spectrum detection technology.

Description

technical field [0001] The invention relates to a kit for detecting human diabetes sensitive genes, belonging to the technical field of biomedicine. Background technique [0002] Pharmacogenomics studies have shown that the efficacy and metabolism of diabetes-related drugs (such as metformin, glibenclamide, tolbutamide, etc.) are closely related to genes such as C11orf 65, CYP2C9, TCF7L2, ABCC8, and KCNJ11 in the human genome; Individuals with related gene mutations are likely to cause symptoms such as poor drug efficacy or even side effects during the course of medication; therefore, it is of great guiding significance for patients to detect the mutations of related genes before using such drugs. [0003] At present, the drug metabolism gene detection technology of diabetes in the market is mostly carried out by fluorescent PCR and other technologies; the detection throughput is small and the detection cost is high. [0004] The present invention aims to check the variatio...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12Q1/6858
CPCC12Q1/6858C12Q1/6883C12Q2600/106C12Q2600/156C12Q2600/16C12Q2531/113C12Q2537/143C12Q2533/101C12Q2565/627
Inventor 田军龙张为
Owner 为康(苏州)基因科技有限公司
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