Multi-gene detection kit for anti-epileptic drug medication guidance and use method thereof

Abstract

The invention discloses a multi-gene detection kit for anti-epileptic drug medication guidance and a use method thereof. A multi-PCR and fragment length / mass analysis method is used for synchronously,quickly and quantitatively detecting eight single nucleotide polymorphism (SNP) loci on four genes, including CYP2C9, CYP2C19, POLG and UGT1A4, related to anti-epileptic drug metabolism, transfer andtarget site actions. Detection includes the steps that 1, oral cast-off cells are collected and stored in a cell acquisition card or a blood sample is collected and nucleic acid is extracted; 2, thecell acquisition card or the nucleic acid in step 1 serves as a template for multi-PCR amplification; 3, according to the length / mass of PCR product fragments, the eight SNP loci, three personal genome DNA reference (huDNA) genes and a PCR product of a PCR reaction internal reference are synchronously separated; 4, results are subjected to analysis and interpretation. The kit has the advantages ofbeing quick to use, high in sensitivity, good in repeatability, high in specificity, high in flux and low in cost.

Description

Technical field [0001] The invention relates to a multiple gene detection kit, in particular to a multiple gene detection kit used for guiding antiepileptic drugs and a method of use thereof. Background technique [0002] Epilepsy (epilepsy) is a chronic disease with recurring attacks, protracted course, and sudden brain dysfunction. The clinical manifestations are motor, sensory, consciousness and autonomic dysfunction. Epilepsy, as the second largest disease in neurology after cerebrovascular disease, is a serious threat to human health. According to statistics from the World Health Organization (WHO), there are currently about 50 million people worldwide suffering from epilepsy, 80% of whom are in developing countries, and about three-quarters of them do not receive the necessary treatment . The prevalence of epilepsy in my country is 7‰, which is close to the incidence rate of 7.2‰ in developing countries reported by WHO. [0003] Taking anti-epileptic drugs is the main mean...

AI Technical Summary

Problems solved by technology

However, the Sanger sequencing method is complex and expensive

When there are many sequencing sites, it needs to be sequenced one by one, which takes a long time and the cumulative price is relatively expensive

The next-generation sequencing technology realizes sequencing while synthesizing, which has the advantages of high throughput and high efficiency. However, the next-generation sequencing platform is expensive and has low popularity, and its clinical application as a SNP detection technology is not mature enough.

[0014] Due to the large number of gene SNPs related to antiepileptic drug use, the above techniques have obvious limitations, so it is difficult to apply antiepileptic drug use to guide multiple gene detection

[0015] At present, there is no report on the multiple gene detection scheme for antiepileptic drug medication guidance based on multiplex PCR and CE separation technology in China

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