Multi-gene detection kit for anti-epileptic drug medication guidance and use method thereof

A technology for anti-epileptic drugs and medication guidance, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc. It can solve the problems of long time consumption, high cumulative price, high cost, etc., and avoid false negatives and false positives, repeatability Good, high-sensitivity effect

Inactive Publication Date: 2019-05-31
NINGBO HEALTH GENE TECHNOLOGIES CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the Sanger sequencing method is complex and expensive
When there are many sequencing sites, it needs to be sequenced one by one, which takes a long time and the cumulative price is relatively expensive
The next-generation sequencing technology realizes sequencing while synthesizing, which has the advantages of high throughput and high efficiency. However, the next-generation sequencing platform is expensive and has low popularity, and its clini

Method used

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  • Multi-gene detection kit for anti-epileptic drug medication guidance and use method thereof
  • Multi-gene detection kit for anti-epileptic drug medication guidance and use method thereof
  • Multi-gene detection kit for anti-epileptic drug medication guidance and use method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] In this embodiment, the oral exfoliated cells of a patient with epilepsy are collected, the multiple PCR reaction is directly performed using the cell acquisition card as a template, and the sample is finally separated by capillary electrophoresis. The specific steps are as follows:

[0046] 1. Production of multiple gene detection kits for the guidance of antiepileptic drugs, including the following components:

[0047] 1) Epi Primer Mix;

[0048] 2) PCR reaction solution;

[0049] 3) Positive control substance Epi POS;

[0050] 4) Ultra-pure water.

[0051] 2. Collect samples

[0052] Use buccal swabs to collect human oral exfoliated cells and store them on a cell collection card.

[0053] 3. Carry out PCR reaction with cell collection card as template

[0054] 1) Add reagents and samples to the 96-well sample plate / PCR eight-strip tube according to Table 4.

[0055] Table 4 PCR reaction system

[0056] Reagent

Volume (μL) / well

PCR reaction solution

14

Primer composition

2

C...

Embodiment 2

[0076] In this embodiment, a whole blood sample of a patient with epilepsy is collected and nucleic acid is extracted. The extracted nucleic acid is used as a template to perform multiple PCR reaction, and finally the sample is separated by capillary electrophoresis. The specific steps are as follows:

[0077] 1. Production of multiple gene detection kits for the guidance of antiepileptic drugs. The components of the kit are as described in Example 1.

[0078] 2. Collect samples

[0079] Collect a whole blood sample from a patient with epilepsy and extract nucleic acid.

[0080] 3. Use the extracted nucleic acid as a template for PCR reaction

[0081] 1) Add reagents and samples to the 96-well sample plate / PCR eight-strip tube according to Table 9.

[0082] Table 9 PCR reaction system

[0083]

[0084]

[0085] 2) Mix and centrifuge the prepared PCR system to perform PCR reaction. The PCR procedure is as described in Example 1.

[0086] 3) Capillary electrophoresis to separate samples, the...

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PUM

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Abstract

The invention discloses a multi-gene detection kit for anti-epileptic drug medication guidance and a use method thereof. A multi-PCR and fragment length/mass analysis method is used for synchronously,quickly and quantitatively detecting eight single nucleotide polymorphism (SNP) loci on four genes, including CYP2C9, CYP2C19, POLG and UGT1A4, related to anti-epileptic drug metabolism, transfer andtarget site actions. Detection includes the steps that 1, oral cast-off cells are collected and stored in a cell acquisition card or a blood sample is collected and nucleic acid is extracted; 2, thecell acquisition card or the nucleic acid in step 1 serves as a template for multi-PCR amplification; 3, according to the length/mass of PCR product fragments, the eight SNP loci, three personal genome DNA reference (huDNA) genes and a PCR product of a PCR reaction internal reference are synchronously separated; 4, results are subjected to analysis and interpretation. The kit has the advantages ofbeing quick to use, high in sensitivity, good in repeatability, high in specificity, high in flux and low in cost.

Description

Technical field [0001] The invention relates to a multiple gene detection kit, in particular to a multiple gene detection kit used for guiding antiepileptic drugs and a method of use thereof. Background technique [0002] Epilepsy (epilepsy) is a chronic disease with recurring attacks, protracted course, and sudden brain dysfunction. The clinical manifestations are motor, sensory, consciousness and autonomic dysfunction. Epilepsy, as the second largest disease in neurology after cerebrovascular disease, is a serious threat to human health. According to statistics from the World Health Organization (WHO), there are currently about 50 million people worldwide suffering from epilepsy, 80% of whom are in developing countries, and about three-quarters of them do not receive the necessary treatment . The prevalence of epilepsy in my country is 7‰, which is close to the incidence rate of 7.2‰ in developing countries reported by WHO. [0003] Taking anti-epileptic drugs is the main mean...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883
Inventor 吕军英王凡吴勇余丁
Owner NINGBO HEALTH GENE TECHNOLOGIES CO LTD
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