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Detection of curative effect of hypoglycemic drug

A technology for lowering blood sugar and drugs, applied in the fields of molecular biology and medicine, which can solve the problems of zero and low frequency of occurrence

Inactive Publication Date: 2010-12-29
XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Although the CYP2C9*2 allele has a great influence on the enzyme activity, which is only less than *3, the study found that the frequency of this allele in Chinese is very low, almost zero

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0024] Example The use of detection kits

[0025] Step 1: Extraction of DNA template

[0026] Genomic DNA of oral epithelial cells was extracted by silica gel adsorption.

[0027] Step 2: Real-time quantitative PCR reaction

[0028] Use the fluorescent quantitative PCR kit in the detection kit to perform 2 independent fluorescent quantitative PCR reactions. The total volume of each reaction is 10 μl, including 2 μl of DNA template with a concentration of 20 ng / μl, 1 μl of 10X fluorescent quantitative PCR reaction buffer , 0.1μl 25mM dNTP mixture, 0.6μl 25mM MgCl 2 Solution, 0.025 μl (5 units / μl) Taq DNA polymerase, 0.225 μl each of 20 μM sense primer and antisense primer, 0.25 μl each of 10 μM fluorescent probe with VIC and fluorescent probe with FAM, 5.325 μl of deionized water.

[0029] The reaction was carried out on a PCR amplification instrument, and the reaction conditions were 50° C. for 2 minutes, 95° C. for 10 minutes, and 60 cycles of 95° C. for 30 seconds and 60°...

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PUM

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Abstract

The invention discloses a kit for detecting the curative effect of an individual hypoglycemic drug. The kit comprises a specific primer pair, a specific fluorescent probe pair, a fluorescence quantitative PCR conventional assembly and the like, and the specific primer pair is used for detecting a medication target site, namely a CYP2C9 gene and CYP2C19 gene polymorphism site of the hypoglycemic drug. The kit can be used for estimating the curative effect of the individual hypoglycemic drug by detecting the medication target site, namely the CYP2C9 gene and CYP2C19 gene polymorphism site of the hypoglycemic drug.

Description

technical field [0001] The present invention relates to the fields of molecular biology and medicine. More specifically, the present invention relates to a kit for detecting the curative effect of individual hypoglycemic drugs. Assess the efficacy of individual hypoglycemic medications. Background technique [0002] Diabetes mellitus is a metabolic disease related to abnormal insulin production and action and characterized by hyperglycemia, including type 1 diabetes mellitus (T1DM) mainly characterized by absolute insulin deficiency and type 2 diabetes mellitus mainly characterized by relative insulin deficiency and insulin resistance. There are two types of type 2 diabetes mellitus (T2DM). At present, diabetes has become the third major chronic disease that seriously endangers human health after cardiovascular and cerebrovascular diseases and tumors in the world. Type 2 diabetes is the main component of the diabetic population in my country, accounting for more than 90% o...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N21/64
Inventor 冯哲民邹祖烨
Owner XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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