Juno<TM>-based safety medication detection kit for children and chip

Abstract

The invention discloses a Juno<TM>-based safety medication detection kit for children and a chip. The kit and the chip comprise primers at the following loci: HLA-DRB1*15:01-DQB1*06:02 loci of an HLAgene, an HLA-B*57:01 locus of the HLA gene, an HLA-B*15:02 locus of the HLA gene, an HLA-B*58:01 locus of the HLA gene, a rs267606617 locus of an MT-RNR1 gene,a rs267606618 locus of the MT-RNR1 gene,a rs267606619 locus of the MT-RNR1 gene, a rs1057910 locus of a CYP2C9 gene, a rs4244285 locus of a CYP2C19 gene, a rs4986893 locus of the CYP2C19 gene, a rs1065852 locus of a CYP2D6 gene, and a rs2242480 locus of a CYP3A4 gene.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a Juno-based TM Children's safety drug detection kit and chip. Background technique [0002] The irrational use of drugs is very common. Every year in my country, 2.5 million patients are hospitalized due to severe adverse drug reactions, and more than 500,000 people die each year, which causes a huge burden of medical expenses and social and economic burdens on the country. In children, the phenomenon of irrational drug use is even more serious. The incidence of adverse drug reactions in children in my country is 12.5%, twice that of adults. Among the more than 1 million deaf-mute children, about 50% are drug-induced deafness. With the implementation of the two-child policy, the safe drug use of children will face continuous challenges. According to incomplete statistics, from May 2014 to June 2016, national regulatory authorities issued as many as 13 various policy documents d...

AI Technical Summary

Problems solved by technology

Disadvantages: Higher requirements for the sequences on both sides of the SNP site, and the existence of special sequences, such as SNP sites, indels, etc. will affect the accuracy

In addition, for the sample quality is slightly high, it is more troublesome to supplement the data in the later stage. If the sample quality is not very good, it cannot be used; the number of SNP sites that can be detected in a single reaction is between 20-40, and the sites that exceed this number need to be distributed In different Panels, the cost is increased

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