The invention relates to the fields of foods and food processing, in particular to beef balls and a preparation method thereof. According to the beef balls disclosed by the invention, beef, butter, isolated protein, starch, zinc gluconate, docosahexenoic acid, a yeast extract, onion powder, olive oil, table salt, white granulated sugar, a dark soy sauce, cooking wine, pricklyash peel powder, chili powder, pepper powder, monosodium glutamate, sodium bicarbonate, phosphate, glutamine aminopherase and D-erythorbic are used as raw materials; and the beef balls are prepared through the technologies of treating the raw materials, performing injection, performing superfine crushing, performing mixing, performing pickling, performing shaping, performing cooking, performing quick-freezing and the like. Through the adoption of the technology disclosed by the invention, zinc and the docosahexenoic acid are uniformly added to the beef balls, the total intaking quantity of human bodies to the docosahexenoic acid can be accurately calculated and controlled through the eating quantity of the beef balls, and finally the purpose of reasonably complementing the zinc and the docosahexenoic acid is realized.