Improved methods for fermentative production of docosahexaenoic acid

A technology for fermentation liquid and fermentation tank, which is applied in the field of fermentation production of docosahexaenoic acid, and can solve the problems of optimizing productivity and controlling pollution.

Inactive Publication Date: 2012-01-25
V B MEDICARE PVT LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0021] However, fed-batch fermentation methods are cumbersome in practice in terms of optimizing productivity and controlling contamination

Method used

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  • Improved methods for fermentative production of docosahexaenoic acid
  • Improved methods for fermentative production of docosahexaenoic acid
  • Improved methods for fermentative production of docosahexaenoic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Solid state fermentation to produce DHA

[0060] Mix 30ml of water with 100g of coarse Ragi (finger millet) powder and autoclave. 2 g of glucose, 2 g of glycerol and 0.02 g of amylase were dissolved in 100 ml of water and autoclaved. Inoculate 10 ml of the seed solution prepared in Example 1 into 100 ml of aqueous medium, and mix thoroughly in a vortex mixer. Pour the contents into the sterilized Ragi powder and mix well. The mixed solid matrix was spread on trays in a layer thickness of 3 mm. Place the tray in an incubator with sterile gas, and control the temperature below 26°C for 120 hours.

[0061]Remove the tray, spread out the solid medium from the tray, mix well and grind. The ground solid was loaded onto a column containing methanol. The solid matrix was thoroughly soaked in methanol for 16 hours, and the methanol was collected. Re-extraction with methanol was performed by soaking for 8 hours, and the methanol layer was collected. The methanol layers wer...

Embodiment 2

[0063] Production of DHA by Solid State Fermentation ATCC PRA 148

[0064] (A) Starting seed culture - Thraustochytrid ATCC PRA 148

[0065] 100 ml of seed medium was prepared in a 250 ml Erlenmeyer flask containing 100 ml of water, 5 g of glucose, 0.5 g of yeast extract, 3.5 g of soy flour and 4.0 g of sea salt. Adjust the pH to 5 and autoclave. A full circle of Thraustochytrium ATCC PRA 148 was transferred to the flask and incubated at 22° C., 220 RPM for 72 hours.

[0066] (B) Solid-state fermentation to produce DHA

[0067] Mix 50ml of water with 100g of coarse rice flour and autoclave. 2 g of glucose, 2 g of glycerin, 3 g of soy peptone and 0.02 g of amylase were dissolved in 100 ml of water and autoclaved. Inoculate 10 ml of the seed solution prepared in Example 1 (A) into 100 ml of aqueous medium, and mix thoroughly in a vortex mixer. Pour the contents into the sterilized rice and mix well. The mixed solid matrix was spread on trays in a layer thickness of 3 mm. ...

Embodiment 3-

[0069] Example 3 - Fermentation of sucrose

[0070] (A) Starting seed culture - Schizochytrium limacinum ATCC MYA 1381

[0071] 100 ml of seed medium was prepared in a 250 ml Erlenmeyer flask containing 100 ml of water, 0.2 g of glucose, 2 g of sucrose, 0.25 g of yeast extract, 4 g of soybean flour and 3.2 g of sea salt. Adjust the pH to 7.5 with 1 N NaOH and autoclave. A full circle of Schizochytrium ATCC MYA 1381 was transferred to the flask and cultured at 22° C. and 220 RPM for 48 hours.

[0072] Prepare a 750 ml seed culture in a 1000 ml Erlenmeyer flask containing 750 ml water, 1.5 g glucose, 15 g sucrose, 7 g starch, 2 g bacterial amylase, 1.125 g yeast extract, 30 g soybean flour, 1.125 g diammonium phosphate and 24 g sea salt base. Adjust the pH to 7.5 with 1 N NaOH and autoclave. 7.5 ml of seed solution prepared from a 100 ml seed flask was inoculated therein. The flask was placed in a shaker and incubated at 24°C for 58 hours.

[0073] (B) Submerged batch ferm...

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Abstract

The invention comprises a process of producing polyunsaturated fatty acids from Thraustochytriales at high availability of oxygen by solid state fermentation or submerged fermentation in a batch, semi-continuous or continuous mode. Batch fermentation of more than 200g / L and up to 450 g / L of dextrose added right in the beginning of fermentation was achieved with productivity of DHA achieved for higher than 10g / Liter. The process comprises maintaining at least 20% to 30% dissolved oxygen and sometimes up to around 50% or more.

Description

technical field [0001] The present invention relates to an improved method for fermentatively producing docosahexaenoic acid. More particularly, it relates to medium compositions containing soybean meal and other nutrients for promoting fat production, and more particularly, to the batch-wise production of bacteria from Thraustochytriales in liquid as well as solid medium Polyunsaturated fatty acids produced by fermentation. The present invention also relates to a new method for producing docosahexaenoic acid by fermentation. Background technique [0002] Essential fatty acids refer to those fatty acids that are essential for the health of an organism, but cannot be synthesized by animals / humans and must be provided through food sources. Omega-6 fatty acids and omega-3 fatty acids are examples of these fatty acids, which play critical roles in brain function and normal growth and development. These two essential fatty acids are polyunsaturated fatty acids (PUFAs) and are ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/40C12P7/64
CPCC12P7/6427
Inventor 奈克旭·拉特兰桑迪普·奥罗拉苏伦德拉·塔卢里艾拉潘·甘尼森维尼塔·皮特
Owner V B MEDICARE PVT LTD
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