37 results about "Protist" patented technology

The present invention is directed to the isolation and use of super-infective, tumor-specific vectors that are strains of parasites including, but not limited to bacteria, fungi and protists. In certain embodiments the parasites include, but are not limited to, the bacterium Salmonella spp., such as Salmonella typhimurium, the bacterium Mycobacterium avium and the protozoan Leishmania amazonensis. In other embodiments, the present invention is concerned with the isolation of super-infective, tumor-specific, suicide gene-containing strains of parasites for use in treatment of solid tumors.

The present invention is directed to the isolation and use of super-infective, tumor-specific vectors that are strains of parasites including, but not limited to bacteria, fungi and protists. In certain embodiments the parasites include, but are not limited to, the bacterium Salmonella spp., such as Salmonella typhimurium, the bacterium Mycobacterium avium and the protozoan Leishmania amazonensis. In other embodiments, the present invention is concerned with the isolation of super-infective, tumor-specific, suicide gene-containing strains of parasites for use in treatment of solid tumors.

The present invention relates to nucleic acid sequences encoding a hydroxyphenylpyruvate dioxygenase (EC 1.13.11.27, abbreviated herein as HPPD) obtained from protists belonging to the family Blepharismidae, as well as the proteins encoded thereby, and to a chimeric gene which comprises such nucleic acid sequence, and to the use of such nucleic acid sequences, proteins or chimeric genes for obtaining plants which are tolerant to HPPD inhibitor herbicides.

Provided herein are constructs for genome editing or genetic engineering in fungi or protists, methods of using the constructs and media for use in selecting cells. The construct include a polynucleotide encoding a thymidine kinase operably connected to a promoter, suitably a constitutive promoter; a polynucleotide encoding an endonuclease operably connected to an inducible promoter; and a recognition site for the endonuclease. The constructs may also include selectable markers for use in selecting recombinations.

A method of enhancing plant growth comprises the step of introducing an alkane into a location adjacent to a plant. The alkane can be introduced intermittently, and can be combined with another gas and / or nutrients. The alkane preferably comprises a butane substrate. The butane substrate can stimulate the growth of butane-utilizing bacteria, such as Aeromonas caviae, Stenotrophomonas maltophilia, Micrococcus varians, Aureobacterium esteroaromaticum, Aureobacterium barkeri, Rhodococcus fascians, Nocardia paradoxus, Comamonas acidovorans and Pseudomonas aeruginosa. The alkane can increase the amount of heterotrophic bacteria in the location adjacent to the plant, and thereby accelerate a heterotrophic nitrification process. The butane substrate can also stimulate the growth of butane-utilizing fungi. The method can also enhance the growth protists and / or prokaryotes. A system for enhancing plant growth in accordance with the method is also disclosed.

Provided herein are constructs for genome editing or genetic engineering in fungi or protists, methods of using the constructs and media for use in selecting cells. The construct include a polynucleotide encoding a thymidine kinase operably connected to a promoter, suitably a constitutive promoter; a polynucleotide encoding an endonuclease operably connected to an inducible promoter; and a recognition site for the endonuclease. The constructs may also include selectable markers for use in selecting recombinations.

Apparatus and methods are provided for introducing an alkane to a plant in order to stimulate the plant's growth. Any type of plant may be treated, including potted plants, shrubs, trees, lawns, agricultural crops and the like. In a preferred embodiment, the alkane comprises butane, but other compounds can be used, including methane, ethane and propane. The alkane may be combined with water or another liquid carrier, or plant growth-enhancing additives such as nutrients, insecticides and alkane-utilizing bacteria. To induce aerobic conditions, oxygen-containing gas such as air may be introduced along with the alkane or separately from the alkane. The introduction of alkane may enhance plant growth by increasing the indigenous microbial populations in soil, e.g. bacteria, fungi, protists, and prokaryotes, which may provide benefits such as increased nutrient uptake, faster root development, and reduced heat, drought and cold stress, resulting in enhanced plant growth.

Methods and means for efficiently downregulating the expression of a target gene of interest in cell from an organism that is an animal, fungus and protist. The invention provides chimeric nucleic acid molecules for downregulating target genes. The invention also provides modified cells and organisms comprising the chimeric nucleic acid molecules and compositions comprising the chimeric molecules.

The present invention relates to a method for enhancing levels of polyunsaturated fatty acids in thraustochytrid protists and more particularly to a method of enhancing levels of docosahexaenoic acid and eicosapentaenoic acid in cells of thraustochytrid protist belonging to the genera Schizochytrium, Thraustochytrium and Aplanochytrium deposited at The Microbial Type Culture Collection (MTCC), Institute of Microbial Technology, Chandigarh, India under the accession numbers MTCC 5121, MTCC 5122 and MTCC 5123 respectively by growing the same in a medium with increased viscosity, whereby the cells thus enriched in the said polyunsaturated fatty acids (PUFAs) can then be utilized successfully in various beneficial applications that require polyunsaturated fatty acids, such as in animal feeds, human nutrition and extraction of the PUFAs for nutritional supplementation.

The invention discloses schizochytrium limacinum named as Aurantiochytrium sp, and preserved in China General Microbiological Culture Collection Center (CGMCC) located at No. 3, courtyard 1, the western Beichen road, Chaoyang District of Beijing on Nov. 2nd, 2017, and the preservation number is CGMCC No:14849. The schizochytrium limacinum can be used for preparing DHA, docosapentaenoic acid greaseand squalene. The strain can resist high fermentation temperature, and the fermentation cost can be effectively lowered; the strain can rapidly grow under the condition that the temperature is 33-37DEG C, a large amount of polyunsaturated fatty acids (DHA and docosapentaenoic acid) and squalene grease can be accumulated, the growth speed is high, and the yield of polyunsaturated fatty acid grease is high.

The invention discloses a method for determining microbial species in a sample. The method comprises the following steps: sequencing at least one part of nucleic acids in the to-be-detected sample, so as to obtain sequencing data; performing alignment of the sequencing data with a first reference sequence, wherein the first reference sequence is a known sequence which is derived from the to-be-detected sample and belongs to an individual organism category, and acquiring a read which meets at least one of (1)-(3), wherein (1) the read is not subjected to alignment with the first reference sequence, (2) the similarity, with the first reference sequence, of the read is less than a preset proportion, and (3) the read is subjected to alignment with the first reference sequence and alignment quality is lower than a threshold; and performing alignment of the obtained read with a microbial reference sequence, and determining the microbial species in the to-be-detected sample in accordance with an acquired alignment result, wherein the microbial reference sequence includes at least two of bacterium, fungus, virus and protist reference sequences. The invention also discloses a device for determining the microbial species in the sample and a method for detecting pathogenic microorganisms.

Methods of detecting and treating rapidly growing exogenous cells, such as Protista, or parasites, that preferentially accumulate a photoactivatable porphyrin in which 5-aminolevulinic acid or precursor thereof is administered to the patient, or contacted to the exogenous cells, in an amount sufficient to induce synthesis fluorescence and / or photosensitizing concentrations of a protoporphyrin IX in the exogenous cells, followed by exposure of the exogenous cells to light of photoactivating wavelengths.

The invention provides a filter composite layer and a filter blocking net, and application method thereof. The composite filter layer comprises a filter layer and a reinforcement layer. The filter layer is a pure polyester or polypropylene short fiber spunlaced non-woven fabric. The reinforcement layer is a terylene industrial filament interlacing netted woven fabric or warp knitted fabric. The reinforcement layer is arranged in the filter layer. The reinforcement layer and the filter layer are combined together as one spunlaced layer. The filter composite layer and filter blocking net, and application method thereof provided by the invention can effectively clean duckweed, algae and other protist on surface of drinking water reservoir and urban river and lake, so as to avoid water pollution caused by aquatic protist and to ensure water quality safety.

A method of enhancing plant growth comprises the step of introducing an alkane into a location adjacent to a plant. The alkane can be introduced intermittently, and can be combined with another gas and / or nutrients. The alkane preferably comprises a butane substrate. The butane substrate can stimulate the growth of butane-utilizing bacteria, such as Aeromonas caviae, Stenotrophomonas maltophilia, Micrococcus varians, Aureobacterium esteroaromaticum, Aureobacterium barkeri, Rhodococcus fascians, Nocardia paradoxus, Comamonas acidovorans and Pseudomonas aeruginosa. The alkane can increase the amount of heterotrophic bacteria in the location adjacent to the plant, and thereby accelerate a heterotrophic nitrification process. The butane substrate can also stimulate the growth of butane-utilizing fungi. The method can also enhance the growth protists and / or prokaryotes. A system for enhancing plant growth in accordance with the method is also disclosed.

The invention features devices and kits for capturing and culturing microorganisms (e.g., bacteria, fungi, or protists) and methods of using the devices and kits to detect microorganisms in environmental and other samples. The device includes a nutrient media having a flat growth area on which microorganisms can grow. Samples are collected by contacting the device with any environmental sample, e.g., rolling device on a work surface or exposing device to air, or by filtering a sample through a membrane. Microorganisms deposited on the membrane derive nutrients from the underlying media and grow into colonies that can then be detected using methods known in the art. The detected colonies can be imaged digitally or with film.

The invention discloses a prawn and grass symbiotic culture method. The prawn and grass symbiotic culture method comprises the following steps that S1, a 6 x 3 x 3 natural pond is built, water is dissolved with quicklime, the quicklime is uniformly sprayed to disinfect, the whole pond is disinfected with niclosamide 340 after 10 days, disinfecting is conducted for 5 days, a water inlet valve is arranged, aquatic plants with stem length of more than 10cm is selected, the aquatic plants is planted on a floating mat, continuously culturing is conducted for 3-4 days, and prawn seedlings are placed; and S2, dissolved oxygen content, ammonia content and nitrogen content of water are monitored, and the number of prawns and the aquatic plants and shelling condition, exercise amount and food intake of the prawns are observed by using an underwater camera, a reinforcement learning algorithm is adopted, and according to the dissolved oxygen content, the ammonia content and the nitrogen content of the water and the number of the prawns and the aquatic plants, the putting amount and the illumination degree of drugs or fertilizer and water grass culture products for inhibiting protists and oxygenates are added. According to the prawn and grass symbiotic culture method, a deep learning model and a reinforcement learning model are used for automatic dynamic adjustment, macroscopic regulation and control and real-time adjustment are achieved, manual control is not needed, and aquaculture achievement of the prawns and the aquatic plants can be greatly improved.

Provided herein are constructs for genome editing or genetic engineering its fungi or protists, methods of using the constructs and media for use in selecting cells. The construct include a polynucleotide encoding a thymidine kinase operably connected to a promoter, suitably a constitutive promoter; a polynucleotide encoding an endonuclease operably connected to an inducible promoter; and a recognition site for the endonuclease. The constructs may also include selectable markers for use in selecting recombinations.

The invention discloses a method for preparing an animal feed or a fertilizer by taking a mixture of one or more bacteria and fungi, seaweed and protist as strain to ferment various crops waste and the excrement of livestock and poultry.

The present invention provides a method for introducing a functional peptide encoded by a plant or protist nucleic acid sequence into a mitochondrion of a mammalian cell, and a pharmaceutical composition comprising the nucleic acid sequence. The present invention also provides a method for correcting a phenotypic deficiency in a mammal resulting from a mutation in a mitochondrial peptide. Additionally, the present invention is directed to a method for treating a mitochondrial disorder in a subject in need of treatment therefor. The present invention further provides expression vectors for use in introducing a functional peptide encoded by a plant or protist (including algal) nucleic acid sequence into a mitochondrion of a mammal, as well as mammalian cells transformed by the expression vectors. Also provided are clonal cell strains comprising the transformed mammalian cells. Finally, the present invention is directed to a method for introducing a functional peptide into a mitochondrion.

Methods of detecting and treating rapidly growing exogenous cells, such as Protista, or parasites, that preferentially accumulate a photoactivatable porphyrin in which 5-aminolevulinic acid or precursor thereof is administered to the patient, or contacted to the exogenous cells, in an amount sufficient to induce synthesis fluorescence and / or photosensitizing concentrations of a protoporphyrin IX in the exogenous cells, followed by exposure of the exogenous cells to light of photoactivating wavelengths.

A method for producing an animal feed has the steps of: cultivating funguses including actinomycess, deotrichum candidum, cellulose mono-saturated bacteria, lactic ferments, phycolmycete, protist, tirchoderma, saccharomyces cerevisiae, candida utilis, and aspergillus niger; producing fermenter by cultivating funguses to be fermented and then drying the cultivated funguses, and then by mixing the cultivated and dried funguses with sodium chloride, catalyzer and calcium oxide; mixing the produced fermenter with a raw material for the animal feed, the raw material being a mixture of one or more of grain, stem of field crop, power of plants, feces of livestock, waste of industrial ferments; and putting the mixture of the raw material and the fermenter in a sealed container, the sealed container being formed of different types of plastic film in multiple layers, and has a check valve formed on one side.

The present application discloses a targeting composition that actively targets chitin-like substances, such as those found in fungi, protists, arthropods, Alzheimer's plaques, etc. Methods are disclosed for preparation of chitin-targeted drug delivery vehicles composed of the targeting composition and one or more bioactive compounds.

This invention provides a cellulase enzyme derived from intestinal symbiotic protists of insects selected from the group consisting of Reticulitermes speratus, Hodotermopsis sjostedti, Neotermes koshunensis, Mastotermes darwiniensis, and Cryptocercidae and comprising an amino acid sequence encoded by a nucleotide sequence selected from the group consisting of the nucleotide sequences as shown in SEQ ID NOS:1 to 140 and nucleotide sequences having 90% or higher identity therewith, a mixture of two or more said enzymes, a processed matter of said enzyme or mixture, DNA encoding said enzyme and an expression system thereof, and a method for producing said enzyme.

Methods and compositions for controlling termite populations by leveraging newly discovered mechanisms for symbiont- and host mediated anti-fungal defenses in the termite hind-gut. Such methods may include administering a composition to a termite, the composition formulated to promote protist dysbiosis in the gut of the termite, or silencing specific host or symbiont genes by RNA interference, and exposing the termite to a pathogen, where the composition increases the susceptibility of the termite to the pathogen. Compositions are also provided for the treatment of fungal infections, the composition comprising a medically effective amount of recombinant glycosyl hydrolase family 7 cellulase, as well as methods for utilizing the same.

This invention discloses a process of production of antiviral polysaccharides derived from extracellular culture filtrate of tharaustaochytrids, belonging to the marine protistan group of Labyrinthulomycetes. The antiviral polysaccharide shows a broad-spectrum antiviral activity, being active against viruses such as the enterovirus, retrovirus, adenovirus and cytomegalovirus.

The object of the present invention is to separate and provide a β-glucosidase gene having the effect of efficiently promoting saccharification in hydrolysis of cellulose-containing biomass from a hardly culturable symbiotic protist community of Coptotermes formosanus, and the present invention specifically relates to β-glucosidase derived from a protist of the genus Pseudotrichonympha consisting of the amino acid sequence represented by SEQ ID NO: 1.

The invention features particle removal assemblies and methods for removing dust and other debris from a sample container, e.g., to improve counting colonies of microorganisms (e.g., bacteria, fungi, or protists) present in environmental, pharmaceutical, biological, and other samples. An assembly of the invention includes components for particle removal, e.g., a filter, a dryer, a flow controller, and an outlet. The invention also provides methods of detecting samples after cleaning a sample container with clean and / or dry gas.