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Ocean protist and method for fermenting and producing high-value-added lipid product

A technology of microbial strains and fermentation medium, which is applied in the field of marine protists and the use of their fermentation to produce high value-added lipid products, can solve the problems of resource exhaustion, difficulty in meeting huge demands, etc. Effect

Active Publication Date: 2018-05-08
青岛洪邦生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, traditional sources, such as marine fish fishing, are exhausted due to overexploitation, and it is difficult to meet the huge growing demand of society.

Method used

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  • Ocean protist and method for fermenting and producing high-value-added lipid product
  • Ocean protist and method for fermenting and producing high-value-added lipid product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Put the strains stored in glycerol tubes into a 250 ml shake flask containing 50 ml seed medium, and culture them in a shaker at 33°C at a speed of 200 rpm for 24 h to obtain first-grade seeds; Insert it into a 500 ml shake flask containing 100 ml of seed medium, and culture it in a shaker at 35°C at a speed of 200 rpm for 12 h to obtain secondary seeds; add 1.2 L of fermentation culture to a 3L bioreactor Base, access to the activated secondary seed solution. During the fermentation process, the temperature was controlled at 35°C. During the fermentation process, nitrogen supplementation was adopted, and 20% (w / v) yeast extract solution was added within 24-48 hours of fermentation. During the fermentation process, the glucose concentration in the fermentation system was maintained at 10-15 g / L by feeding 80% glucose solution. The pH was maintained at 6.5 by automatic addition of 2M NaOH or 14% citric acid. After 65 hours of fermentation, hydrogen peroxide was added ...

Embodiment 2

[0032]Put the strain stored in the glycerol tube into a 250 ml shake flask containing 50 ml of seed medium, and culture it in a shaker at 25°C at a speed of 200 rpm for 24 h to obtain first-grade seeds; Insert 100 ml of seed medium into a 500 ml shake flask, and culture in a shaker at 30°C at 200 rpm for 12 h to obtain secondary seeds; add 1.2 L of fermentation culture to a 3 L bioreactor Base, access to the activated secondary seed solution. During the fermentation process, the temperature was controlled at 33°C. During the fermentation process, the glucose concentration in the fermentation system was maintained at 10-15 g / L by feeding 80% glucose solution. Automatic addition of 2M NaOH or 14% citric acid kept the pH at 6.5. The fermentation time was 90 hours, and 10 hours before the end of the fermentation, hydrogen peroxide was added to make the concentration of hydrogen peroxide in the fermentation system 0.05%.

[0033] The fermentation medium contains: glucose 60 g / L,...

Embodiment 3

[0037] Put the strains stored in glycerol tubes into a 250 ml shake flask containing 50 ml of seed medium, and culture them in a shaker at 33°C at a speed of 200 rpm for 24 hours to obtain first-grade seeds; Put them into a 500ml shake flask with 100ml seed medium, and cultivate them in a shaker at 35°C at a speed of 200rpm for 12h to obtain secondary seeds; add 3L fermentation medium to a 5L bioreactor, and then into the activated secondary seed solution. During the fermentation process, the temperature was controlled at 37°C. During the fermentation process, the glucose concentration in the fermentation system was maintained at 10-15 g / L by feeding 80% glucose solution. The pH was maintained at 6.5 by automatic addition of 2M NaOH or 14% citric acid. The fermentation time was 90 hours, and 10 hours before the end of the fermentation, hydrogen peroxide was added to make the concentration of hydrogen peroxide in the fermentation system 0.01%.

[0038] The fermentation mediu...

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Abstract

The invention discloses schizochytrium limacinum named as Aurantiochytrium sp, and preserved in China General Microbiological Culture Collection Center (CGMCC) located at No. 3, courtyard 1, the western Beichen road, Chaoyang District of Beijing on Nov. 2nd, 2017, and the preservation number is CGMCC No:14849. The schizochytrium limacinum can be used for preparing DHA, docosapentaenoic acid greaseand squalene. The strain can resist high fermentation temperature, and the fermentation cost can be effectively lowered; the strain can rapidly grow under the condition that the temperature is 33-37DEG C, a large amount of polyunsaturated fatty acids (DHA and docosapentaenoic acid) and squalene grease can be accumulated, the growth speed is high, and the yield of polyunsaturated fatty acid grease is high.

Description

technical field [0001] The invention belongs to the field of microbial fermentation engineering, and in particular relates to a marine protist and a method for producing high value-added lipid products by fermentation thereof. Background technique [0002] The ocean accounts for 71% of the earth's surface area, and is rich in characteristic marine biological resources. These characteristic biological resources produce unique and novel bioactive components, and are important strategic new resources for the development of new functional health foods, biological products and drugs. Polyunsaturated fatty acids, astaxanthin, squalene and other natural active lipids mainly come from the ocean. Due to their unique physiological activities, they have many physiological functions on human health, such as: promoting the development of the nervous system and visual system, preventing and treating Cardiovascular diseases, anti-cancer effects, anti-inflammatory effects, anti-oxidation ef...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P7/64C12R1/645
CPCC12P7/6427C12P7/6463C12P7/6472C12N1/145C12R2001/645
Inventor 路延笃
Owner 青岛洪邦生物技术有限公司
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