Preparation method of DHA (

A carbon source and amino acid technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve the effect of avoiding differences in inorganic salt components and being easy to use

Inactive Publication Date: 2010-11-17
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no fully synthetic medium that is specifically designed according to th...

Method used

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  • Preparation method of DHA (
  • Preparation method of DHA (
  • Preparation method of DHA (

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: Effects of Different Carbon Sources on Schizochytrium sp. Fermentation of DHA

[0033] Select 5 kinds of carbon sources (glucose, fructose, sucrose, lactose and glycerol) and add them to the culture medium at a concentration of 120g / L respectively, the nitrogen source is yeast extract, the addition amount is 10g / L, and artificial seawater inorganic salt solution; The filling volume is 100ml / 500ml, cultured at 25°C and 200r / min for 4 days, the results are shown in figure 1 . The five carbon sources Schizochytrium sp. selected as shown in the figure can all be utilized, among which the utilization of glycerol and lactose is relatively difficult, and the utilization rate of glucose, sucrose and fructose is relatively high. With glucose as carbon source, the biomass of Schizochytrium sp. shake flask is 36.1g / L, which is 1.2 times of fructose and 2 times of glycerol; the content of DHA is 6.14g / L. Therefore, glucose is the best carbon source for the growth and r...

Embodiment 2

[0034] Example 2: Nitrogen source - single deletion experiment of amino acid components

[0035] In this example, an amino acid single deletion experiment was designed to screen out amino acid components that have a significant effect on the growth of Schizochytrium sp. On the basis of Example 1, glucose is selected as the carbon source, 20 kinds of basic amino acids are used instead of yeast extract, and the initial concentration of each amino acid is 0.5g / L. Each time one amino acid is missing, the concentration of other amino acids remains unchanged. The results are shown in figure 2 .

[0036] like figure 2 As shown, the 20 basic protein components amino acids have different roles in the process of Schizochytrium sp. growth and accumulation of DHA. The deletion of 10 amino acids, including cysteine, methionine, glutamine, isoleucine, threonine, and tryptophan, had a relatively greater impact on the growth and accumulation of DHA in Schizochytrium sp., which was the sam...

Embodiment 3

[0037] Example 3: Utilization of amino acids during growth of Schizochytrium sp.

[0038] The amino acid components in the medium before and after the growth of Schizochytrium sp. were analyzed by liquid chromatography, and the utilization of various essential amino acids during the growth of Schizochytrium sp. was obtained, as shown in Table 1.

[0039] Table 1 The utilization of amino acids during the growth of Schizochytrium sp.

[0040]

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Abstract

The invention relates to a preparation method of DHA (which relates to an unsaturated fatty acid. The invention provides a preparation method of a fully synthetic medium that is suitable for high density growth of fissiparism chytrid and increases the output of the DHA. The DHA comprises a carbon source, amino acids, lysine, inorganic salts, vitamins, trace elements and water. The carbon source is glucose, the amino acids comprise alanine, methionine, cysteine, lysine, histidine, glutamic acid, glutamine, isoleucine, threonine and tryptophan; the vitamins comprise VB1, VB12, biotin, lipoic acid and folic acid; the inorganic salts comprise NaCl, KCl, MgCl2, CaCl2, Na2HPO4 and KH2PO4; and the trace elements comprise AlCl3, H3BO3, ZnSO4, MnCl2, CuSO4, FeSO4 and (NH4)6Mo7O24.4H2O. The synthetic medium is utilized for culturing the fissiparism chytrid based on a shake flask, and the output of the DHA is 9.8g/L.

Description

technical field [0001] The invention relates to an unsaturated fatty acid, in particular to a method for cultivating marine microorganisms to produce highly unsaturated fatty acids using a fully synthetic medium. Background technique [0002] It has been fully proved that the beneficial effect of increasing the dietary intake of long-chain omega-3 unsaturated fatty acids in the human body, and described DHA is one of the most important long-chain omega-3 unsaturated fatty acids, said beneficial effects include: ( 1) Strengthening the brain and improving intelligence, protecting eyesight: research shows that DHA plays a very important role in the normal development of the brain of infants and the normal function of the adult brain. It can not only promote the development of the brain and eyes of infants, but also improve their cognitive Cognitive ability, and affect adult's brain function and behavioral response, can be used effectively in the treatment of Alzheimer's dementi...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P7/64C12R1/645
Inventor 卢英华倪洁周林陈丽珠敬科举乔兴忠
Owner XIAMEN UNIV
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