Identification method of rice bacterial blight resistance and application of miRNA172a

A technology for bacterial blight resistance and identification method, which is applied to the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve problems such as inaccurate resistance to bacterial blight, and achieves that it is not easy to degrade and avoid misjudgment. , the effect of improving the accuracy

Inactive Publication Date: 2015-07-29
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Abstract
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Problems solved by technology

[0006] In order to solve the shortcomings of using DNA molecular markers to identify whether rice is resistant to bacterial blight is not accurate enough, the embodiment of the present invention provides an identification method of rice bacterial blight resistance and the application of miRNA172a

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  • Identification method of rice bacterial blight resistance and application of miRNA172a
  • Identification method of rice bacterial blight resistance and application of miRNA172a

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Embodiment

[0024] In the present invention, rice miRNA is hybridized with a probe labeled with miRNA172a gene, and the result is detected by non-denaturing polyacrylamide gel electrophoresis, and the results of the rice to be tested and the susceptible rice are compared, and then judged. Whether rice is resistant to bacterial blight, the specific experimental process is as follows.

[0025] Susceptible rice: select non-bacterial blight-resistant rice seeds labeled 9311 with normal growth and no obvious pests and diseases on the surface.

[0026] Rice to be tested: select rice seeds with the label 9311 resistant to bacterial blight.

[0027] Among them, rice seeds resistant to bacterial blight have resistance gene Xa23.

[0028] Pre-treatment of seeds: Soak in 70% alcohol for 2 minutes, then wash twice with deionized water, soak in water at 30°C overnight, and germinate the seeds at a temperature of 30°C. After the buds grow out, sow In the seedling field, the management of the seedling...

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Abstract

The invention discloses an identification method of rice bacterial leaf blight resistance and an application of miRNA172a genetic locus, and belongs to the field of biotechnology. The identification method comprises the following steps: inoculating paddy rice to be detected and infected paddy rice; separating miRNA genes of the paddy rice to be detected and the infected paddy rice; detecting the expressions of the miRNA genes; and determining whether the paddy rice to be detected is resistant to bacterial leaf blight. The invention further discloses the application of the miRNA172a genetic locus in identification of rice bacterial leaf bright resistance. The invention uses the transcription of the miRNA172a gene locus to identify whether paddy rice is resistant to bacterial leaf blight, and achieves success. The transcription of the miRNA172a gene locus provided by the invention is detection based on expression level, so as to avoid misjudgment caused by bacterial leaf blight resistance gene silencing; and the identification method provided by the invention does not use the conventional DNA linkage tracer method, so as to avoid misjudgment caused by untightness of DNA linkage, improve the detection accuracy and avoid unnecessary production loss.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an identification method of rice bacterial blight resistance and the application of miRNA172a. Background technique [0002] Rice is the most important food crop in my country. Bacterial blight is one of the two major diseases of rice. It is a worldwide disease, especially in Asian rice regions. Bacterial blight has a high incidence and rapid infection, and the rice fields with the disease will reduce production by 20-30%, or even 50%. [0003] Breeding and utilizing disease-resistant varieties is the most economical and effective means to solve the grain yield reduction caused by bacterial blight. Therefore, it is necessary to identify the resistance of cultivated rice to bacterial blight. At present, the method for identifying rice bacterial blight usually uses DNA (Deoxyribonucleic acid, deoxyribonucleic acid) molecular markers, and uses the principle of DNA linkage markers to id...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
Inventor 翟文学彭海江光怀高丽芬
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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