MSO/GO (Mercury-Specific Oligonucleotide/Graphene Oxide) based water environment mercury ion detecting method and kit

A detection method and technology of mercury ions, which are applied in the determination/inspection of microorganisms, biochemical equipment and methods, fluorescence/phosphorescence, etc., can solve the problems of complicated and time-consuming operations, unsatisfactory, expensive costs, etc., and achieve convenient and efficient operation. Simple and low-cost effect
CN103173540AInactive Publication Date: 2013-06-26WENZHOU MEDICAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
WENZHOU MEDICAL UNIV
Publication Date
2013-06-26
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to an MSO / GO (Mercury-Specific Oligonucleotide / Graphene Oxide) based mercury ion detecting method and a kit for the rapid detection of mercury ion in water. According to the invention, fluorescence-modified and not-fluorescence-modified optimized MSO is adsorbed to the surface of GO by utilizing the acting force of non-covalent bonds (pi-pi superposition); a structure of T-Hg2+-T is formed by the MSO and the mercury ion to ensure that the fluorescently-labeled MSO is released from the surface of the GO and the fluorescence signal is recovered; and the quantitative determination of the mercury ion in solution is realized through detecting the changes of the fluorescence signal in the solution. The method disclosed by the invention has the advantages that the convenient and fast analysis of the mercury ion can be realized without any special condition; the lowest detection limit is 10pM; the sensitivity is greatly increased; the DNA (DeoxyriboNucleic Acid) synthetizing cost is reduced; a solution which is simple, convenient, efficient, sensitive and cost performance is provided for the monitoring of the pollution of the mercury ion in the water; and the method is easy to popularize.
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Description

technical field

[0001] The invention belongs to the technical field of biological detection, and relates to a method for detecting mercury ions based on mercury-specific oligonucleotide probes (mercury-specific oligonucleotide, MSO) and graphene oxide (Graphene oxide, GO) and a method for detecting mercury in water. A kit for the rapid detection of ions. Background technique

[0002] In recent years, with the rapid development of modern industry and agriculture, environmental pollution and poisoning incidents caused by mercury broke out frequently. The discharge of industrial "three wastes", the production of urban and rural garbage, and the unreasonable use of pesticides have made this "invisible killer" threatening our lives and eroding our bodies. Among them, the problem of mercury pollution in my country's water environment is relatively prominent. According to the survey by the Ministry of Agriculture, the area of ​​sewage irrigation areas in my country is about 140×10...

Claims

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