A miRNA barrier technique

A technology of barrier and technology, applied in the field of biotechnology, can solve the problem of miRNA being negligible, and achieve the effect of stable and high safety of imported genes
CN101054576AInactive Publication Date: 2007-10-17HARBIN MEDICAL UNIVERSITY +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HARBIN MEDICAL UNIVERSITY
Publication Date
2007-10-17
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention relates to miRNA barrier technology, belonging to biological technology. The miRNA technology can be implemented according to the following steps: 1) determining the target gene sequence of combining mRNA 3' UTR and miRNA, 2) synthesizing specific anti-sense oligo-barrier sequence, 3) transducing specific anti-sense oligo-barrier sequence into cell and combining target mRNA to form diploid, which can barrier the target miRNA of mRNA. 5 nucleotides or deoxyadenylic acids positioned at two ends of the specific anti-sense oligo-barrier sequence of the step 2) are locked while ribose ring is bridge-linked by 2' oxygen atom and 4' carbon atom. The inventive method has property of good transduced gene stability, high expression. The present invention proves that it is practical for interfering miRNA function using gene specific mode and provides novel measure for researching miRNA function which enable the selective gene therapy strategy and lay foundation to miRNA gene-specific use.
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Description

technical field

[0001] The present invention relates to a kind of biotechnology. Background technique

[0002] miRNAs are a class of non-coding single-stranded RNA molecules with a length of 21-25 nt, which widely exist in plants and cells from nematodes to humans. MicroRNA (microRNA, miRNA) is a molecule that exists widely and fine-tunes gene expression. The miRNA gene accounts for about 1% of the human genome. It is a class of molecules that are highly conserved in evolution and play an important regulatory role in life. . Most of the target genes of miRNA are genes involved in transcription, signal transduction, and tumorigenesis. Although the role of miRNA is also specific, especially the 2-8 bases at the 5′ end, which specifically target its target gene, but Different from siRNA (smallinference RNA), the specificity of miRNA is not so strong. In organisms, one miRNA often acts on multiple target genes or multiple miRNAs regulate one target gene.

[0003] At present, ...

Claims

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