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A miRNA that Regulates the Expression of the Anti-rust Fungus Infection Gene in Populus nigra

A gene expression, anti-rust technology, applied in the field of miRNA, can solve the problem of ignoring the behind-the-scenes manipulator

Inactive Publication Date: 2016-03-30
NORTHEAST FORESTRY UNIVERSITY
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Problems solved by technology

Current molecular studies on poplar rust resistance mainly focus on some functional genes and proteins that are directly involved in the resistance effect of poplar against fungal infection, while ignoring the behind-the-scenes manipulators that lead to the differential expression of many genes, that is, the genes involved in defense responses. Upstream Regulatory Networks Are a Blind Spot in Research

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  • A miRNA that Regulates the Expression of the Anti-rust Fungus Infection Gene in Populus nigra
  • A miRNA that Regulates the Expression of the Anti-rust Fungus Infection Gene in Populus nigra
  • A miRNA that Regulates the Expression of the Anti-rust Fungus Infection Gene in Populus nigra

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Embodiment 1

[0013] Example 1: Acquisition of miRNA regulating the expression of the anti-rust fungus infection gene of Populus nigra, identification of miRNA target genes and verification of miRNA function.

[0014] (1) RNA extraction and quality identification of Populus small black:

[0015] Inoculate larch-poplar rust fungus on the leaves of small black poplar, take the leaves of seven groups of small black poplar leaves inoculated with larix-poplar rust fungus 2hpi, 6hpi, 12hpi, 24hpi, 3dpi, 5dpi and 7dpi, and divide them into small pieces 100mg of plant tissue from each group was placed in a 1.5ml centrifuge tube treated with DEPC, and quickly placed in liquid nitrogen to freeze for later use. According to the operation manual of miRNeasyKit (purchased from QIAGEN), total RNA was extracted from the leaves of Populus nigra in each group, and the RNA in each group was mixed in equal proportions. Total RNA was extracted from leaves of Populus nigra not inoculated with P. larix-poplaris...

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Abstract

The invention provides a micro-Ribose Nucleic Acid (miRNA) for regulating and controlling the expression of a rustproofing bacterium infected gene of hybrid poplar and use of the miRNA. The miRNA for regulating and controlling the expression of the rustproofing bacterium infected gene of the hybrid poplar and the use of the miRNA have important biological significance and potential application value in poplar disease resistance breeding by virtue of study on the miRNA and the target gene thereof. The nucleotide sequence of the miRNA for regulating and controlling the expression of the rustproofing bacterium infected gene of the hybrid poplar is as shown in SEQ ID NO: 1. The precursor sequence of the miRNA is as shown in SEQ ID NO: 2. The miRNA for regulating and controlling the expression of the rustproofing bacterium infected gene of the hybrid poplar is capable of improving the resistance of the hybrid poplar to the rust disease and is applied to disease resistance breeding. The study on the miRNA by use of genetic engineering methods is capable of laying a foundation for intensively researching and controlling the rust disease infection resistance mechanism of the poplar and improving the resistance of the hybrid poplar to the rust disease.

Description

technical field [0001] The invention relates to a miRNA that regulates the expression of an anti-rust fungus infection gene. Background technique [0002] MicroRNAs (miRNAs) are endogenous non-coding small RNAs (smallRNAs) that can regulate gene expression, mainly by binding to the target gene mRNA or the 3'-untranslated region (3'-untranslated region, 3'-UTR) of the target gene mRNA To degrade the gene mRNA or hinder its translation, thereby controlling gene expression at the post-transcriptional level. The earliest discovered miRNAs were 18-22 nt long RNA molecules complementary to the 3'-UTR of target gene transcripts observed in C. elegans, including the lin-4 and let-7 genes, and the development of C. elegans is regulated by these RNA genes . Subsequently, miRNAs were found in various tissues including nematodes, Drosophila and human, and the report of plant miRNAs began in 2002. With the development of modern biotechnology and molecular biology, the number of discov...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113C12N15/11C12Q1/68
Inventor 王峰李丹蕾王志英邹莉陈俏丽
Owner NORTHEAST FORESTRY UNIVERSITY
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